ID   AACC3_PSEAI             Reviewed;         271 AA.
AC   P29808;
DT   01-APR-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-APR-1993, sequence version 1.
DT   25-MAY-2022, entry version 61.
DE   RecName: Full=Aminoglycoside N(3)-acetyltransferase III;
DE            EC=2.3.1.81;
DE   AltName: Full=ACC(3)-III;
DE   AltName: Full=Aminocyclitol 3-N-acetyltransferase type III;
DE   AltName: Full=Gentamicin-(3)-N-acetyl-transferase;
GN   Name=aacC3;
OS   Pseudomonas aeruginosa.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=287;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=PST-I;
RX   PubMed=1649572; DOI=10.1128/aac.35.5.892;
RA   Vliegenthart J.S., Ketelaar-Van Gaalen P.A.G., van de Klundert J.A.M.;
RT   "Nucleotide sequence of the aacC3 gene, a gentamicin resistance determinant
RT   encoding aminoglycoside-(3)-N-acetyltransferase III expressed in
RT   Pseudomonas aeruginosa but not in Escherichia coli.";
RL   Antimicrob. Agents Chemother. 35:892-897(1991).
CC   -!- FUNCTION: Resistance to antibiotics containing the 2-deoxy-streptamine
CC       ring including dibekacin, gentamicin, kanamycin, sisomicin, tobramycin,
CC       neomycin and to a lesser extent netilmicin.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2-deoxystreptamine antibiotic + acetyl-CoA = an N(3')-
CC         acetyl-2-deoxystreptamine antibiotic + CoA + H(+);
CC         Xref=Rhea:RHEA:12665, ChEBI:CHEBI:15378, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57288, ChEBI:CHEBI:57921, ChEBI:CHEBI:77452; EC=2.3.1.81;
CC   -!- SIMILARITY: Belongs to the antibiotic N-acetyltransferase family.
CC       {ECO:0000305}.
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DR   EMBL; X55652; CAA39184.1; -; Genomic_DNA.
DR   PIR; S18730; S18730.
DR   RefSeq; WP_063840261.1; NG_047241.1.
DR   AlphaFoldDB; P29808; -.
DR   SMR; P29808; -.
DR   KEGG; ag:CAA39184; -.
DR   GO; GO:0046353; F:aminoglycoside 3-N-acetyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   InterPro; IPR003679; Amioglycoside_AcTrfase.
DR   InterPro; IPR028345; Antibiotic_NAT-like.
DR   PANTHER; PTHR11104; PTHR11104; 1.
DR   Pfam; PF02522; Antibiotic_NAT; 1.
DR   SUPFAM; SSF110710; SSF110710; 1.
PE   3: Inferred from homology;
KW   Acyltransferase; Antibiotic resistance; Transferase.
FT   CHAIN           1..271
FT                   /note="Aminoglycoside N(3)-acetyltransferase III"
FT                   /id="PRO_0000068546"
SQ   SEQUENCE   271 AA;  29618 MW;  A66D13D364AD1484 CRC64;
     MTDLNIPHTH AHLVDAFQAL GIRAGQALML HASVKAVGAV MGGPNVILQA LMDALTPDGT
     LMMYAGWQDI PDFIDSLPDA LKAVYLEQHP PFDPATARAV RENSVLAEFL RTWPCVHRSA
     NPEASMVAVG RQAALLTANH ALDYGYGVES PLAKLVAIEG YVLMLGAPLD TITLLHHAEY
     LAKMRHKNVV RYPCPILRDG RKVWVTVEDY DTGDPHDDYS FEQIARDYVA QGGGTRGKVG
     DADAYLFAAQ DLTRFAVQWL ESRFGDSASY G