PSBF_TOBAC
ID PSBF_TOBAC Reviewed; 39 AA.
AC P60124; P05171; P09198; Q95H58; Q9M3L1;
DT 13-AUG-1987, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 2.
DT 03-AUG-2022, entry version 82.
DE RecName: Full=Cytochrome b559 subunit beta {ECO:0000255|HAMAP-Rule:MF_00643};
DE AltName: Full=PSII reaction center subunit VI {ECO:0000255|HAMAP-Rule:MF_00643};
GN Name=psbF {ECO:0000255|HAMAP-Rule:MF_00643};
OS Nicotiana tabacum (Common tobacco).
OG Plastid; Chloroplast.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae;
OC Nicotiana.
OX NCBI_TaxID=4097;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2450682; DOI=10.1007/bf00418129;
RA Carrillo N., Seyer P., Tyagi A., Herrmann R.G.;
RT "Cytochrome b-559 genes from Oenothera hookeri and Nicotiana tabacum show a
RT remarkably high degree of conservation as compared to spinach. The enigma
RT of cytochrome b-559: highly conserved genes and proteins but no known
RT function.";
RL Curr. Genet. 10:619-624(1986).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Bright Yellow 4;
RX PubMed=16453699; DOI=10.1002/j.1460-2075.1986.tb04464.x;
RA Shinozaki K., Ohme M., Tanaka M., Wakasugi T., Hayashida N.,
RA Matsubayashi T., Zaita N., Chunwongse J., Obokata J.,
RA Yamaguchi-Shinozaki K., Ohto C., Torazawa K., Meng B.-Y., Sugita M.,
RA Deno H., Kamogashira T., Yamada K., Kusuda J., Takaiwa F., Kato A.,
RA Tohdoh N., Shimada H., Sugiura M.;
RT "The complete nucleotide sequence of the tobacco chloroplast genome: its
RT gene organization and expression.";
RL EMBO J. 5:2043-2049(1986).
RN [3]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=cv. Petit Havana;
RX PubMed=12655396; DOI=10.1007/s00438-002-0791-1;
RA Swiatek M., Regel R.E., Meurer J., Wanner G., Pakrasi H.B., Ohad I.,
RA Herrmann R.G.;
RT "Effects of selective inactivation of individual genes for low-molecular-
RT mass subunits on the assembly of photosystem II, as revealed by chloroplast
RT transformation: the psbEFLJoperon in Nicotiana tabacum.";
RL Mol. Genet. Genomics 268:699-710(2003).
CC -!- FUNCTION: This b-type cytochrome is tightly associated with the
CC reaction center of photosystem II (PSII), and is essential for PSII
CC function (PubMed:12655396). PSII is a light-driven water:plastoquinone
CC oxidoreductase that uses light energy to abstract electrons from H(2)O,
CC generating O(2) and a proton gradient subsequently used for ATP
CC formation. It consists of a core antenna complex that captures photons,
CC and an electron transfer chain that converts photonic excitation into a
CC charge separation. {ECO:0000255|HAMAP-Rule:MF_00643,
CC ECO:0000269|PubMed:12655396}.
CC -!- COFACTOR:
CC Name=heme b; Xref=ChEBI:CHEBI:60344;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00643};
CC Note=With its partner (PsbE) binds heme. PSII binds additional
CC chlorophylls, carotenoids and specific lipids. {ECO:0000255|HAMAP-
CC Rule:MF_00643};
CC -!- SUBUNIT: Heterodimer of an alpha subunit and a beta subunit. PSII is
CC composed of 1 copy each of membrane proteins PsbA, PsbB, PsbC, PsbD,
CC PsbE, PsbF, PsbH, PsbI, PsbJ, PsbK, PsbL, PsbM, PsbT, PsbX, PsbY, PsbZ,
CC Ycf12, at least 3 peripheral proteins of the oxygen-evolving complex
CC and a large number of cofactors. It forms dimeric complexes.
CC {ECO:0000255|HAMAP-Rule:MF_00643}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane
CC {ECO:0000255|HAMAP-Rule:MF_00643}; Single-pass membrane protein
CC {ECO:0000255|HAMAP-Rule:MF_00643}.
CC -!- DISRUPTION PHENOTYPE: Plants unable to grow photoautotrophically, no
CC detectable PSII activity although under low light (10 umol
CC photons/m(2)/s) on a carbon source plants are green.
CC {ECO:0000269|PubMed:12655396}.
CC -!- SIMILARITY: Belongs to the PsbE/PsbF family. {ECO:0000255|HAMAP-
CC Rule:MF_00643}.
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DR EMBL; X03781; CAA27413.1; -; Genomic_DNA.
DR EMBL; Z00044; CAA77367.1; -; Genomic_DNA.
DR RefSeq; NP_054516.1; NC_001879.2.
DR AlphaFoldDB; P60124; -.
DR SMR; P60124; -.
DR GeneID; 800446; -.
DR KEGG; nta:800446; -.
DR OrthoDB; 1637060at2759; -.
DR GO; GO:0009535; C:chloroplast thylakoid membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0009539; C:photosystem II reaction center; IEA:InterPro.
DR GO; GO:0009055; F:electron transfer activity; IEA:UniProtKB-UniRule.
DR GO; GO:0020037; F:heme binding; IEA:InterPro.
DR GO; GO:0005506; F:iron ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0009767; P:photosynthetic electron transport chain; IEA:InterPro.
DR HAMAP; MF_00643; PSII_PsbF; 1.
DR InterPro; IPR006241; PSII_cyt_b559_bsu.
DR InterPro; IPR006216; PSII_cyt_b559_CS.
DR InterPro; IPR013081; PSII_cyt_b559_N.
DR PANTHER; PTHR33391:SF14; PTHR33391:SF14; 1.
DR Pfam; PF00283; Cytochrom_B559; 1.
DR PIRSF; PIRSF000037; PsbF; 1.
DR TIGRFAMs; TIGR01333; cyt_b559_beta; 1.
DR PROSITE; PS00537; CYTOCHROME_B559; 1.
PE 3: Inferred from homology;
KW Chloroplast; Electron transport; Heme; Iron; Membrane; Metal-binding;
KW Photosynthesis; Photosystem II; Plastid; Thylakoid; Transmembrane;
KW Transmembrane helix; Transport.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000250"
FT CHAIN 2..39
FT /note="Cytochrome b559 subunit beta"
FT /id="PRO_0000200460"
FT TRANSMEM 14..30
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00643"
FT BINDING 18
FT /ligand="heme"
FT /ligand_id="ChEBI:CHEBI:30413"
FT /ligand_note="ligand shared with alpha subunit"
FT /ligand_part="Fe"
FT /ligand_part_id="ChEBI:CHEBI:18248"
FT /note="axial binding residue"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00643"
FT CONFLICT 17
FT /note="I -> V (in Ref. 2; CAA77367)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 39 AA; 4498 MW; 591251852D6E0A03 CRC64;
MTIDRTYPIF TVRWLAIHGL AVPTVFFLGS ISAMQFIQR
