ATGA1_MOUSE
ID ATGA1_MOUSE Reviewed; 218 AA.
AC Q9D8Z6;
DT 10-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 132.
DE RecName: Full=Autophagy-related protein 101;
GN Name=Atg101;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J, and DBA/2J; TISSUE=Bone marrow, Embryo, and Pancreas;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=FVB/N; TISSUE=Salivary gland;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Autophagy factor required for autophagosome formation.
CC Stabilizes ATG13, protecting it from proteasomal degradation.
CC {ECO:0000250|UniProtKB:Q9BSB4}.
CC -!- SUBUNIT: Interacts with ATG13. Associates with a complex composed of
CC ATG13, ULK1 and RB1CC1; the association with this complex requires the
CC presence of ATG13. {ECO:0000250|UniProtKB:Q9BSB4}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q9BSB4}.
CC Preautophagosomal structure {ECO:0000250|UniProtKB:Q9BSB4}. Note=Under
CC starvation conditions, it is localized to puncate structures primarily
CC representing the isolation membrane; the isolation membrane sequesters
CC a portion of the cytoplasm resulting in autophagosome formation.
CC {ECO:0000250|UniProtKB:Q9BSB4}.
CC -!- SIMILARITY: Belongs to the ATG101 family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AK007505; BAB25076.1; -; mRNA.
DR EMBL; AK150298; BAE29450.1; -; mRNA.
DR EMBL; AK151381; BAE30353.1; -; mRNA.
DR EMBL; AK160336; BAE35743.1; -; mRNA.
DR EMBL; AK167951; BAE39952.1; -; mRNA.
DR EMBL; BC016463; AAH16463.1; -; mRNA.
DR CCDS; CCDS27849.1; -.
DR RefSeq; NP_080842.1; NM_026566.2.
DR RefSeq; XP_006521373.1; XM_006521310.3.
DR AlphaFoldDB; Q9D8Z6; -.
DR SMR; Q9D8Z6; -.
DR BioGRID; 212665; 1.
DR ComplexPortal; CPX-380; ULK1-ATG13-RB1CC1-ATG101 autophagy initiation complex.
DR IntAct; Q9D8Z6; 1.
DR STRING; 10090.ENSMUSP00000045474; -.
DR iPTMnet; Q9D8Z6; -.
DR PhosphoSitePlus; Q9D8Z6; -.
DR EPD; Q9D8Z6; -.
DR MaxQB; Q9D8Z6; -.
DR PaxDb; Q9D8Z6; -.
DR PeptideAtlas; Q9D8Z6; -.
DR PRIDE; Q9D8Z6; -.
DR ProteomicsDB; 265165; -.
DR Antibodypedia; 43032; 92 antibodies from 26 providers.
DR DNASU; 68118; -.
DR Ensembl; ENSMUST00000048393; ENSMUSP00000045474; ENSMUSG00000037204.
DR GeneID; 68118; -.
DR KEGG; mmu:68118; -.
DR UCSC; uc007xsw.1; mouse.
DR CTD; 60673; -.
DR MGI; MGI:1915368; Atg101.
DR VEuPathDB; HostDB:ENSMUSG00000037204; -.
DR eggNOG; KOG4493; Eukaryota.
DR GeneTree; ENSGT00390000016511; -.
DR HOGENOM; CLU_110397_0_0_1; -.
DR InParanoid; Q9D8Z6; -.
DR OMA; PWEVWNL; -.
DR OrthoDB; 1539728at2759; -.
DR PhylomeDB; Q9D8Z6; -.
DR TreeFam; TF320996; -.
DR Reactome; R-MMU-1632852; Macroautophagy.
DR BioGRID-ORCS; 68118; 20 hits in 71 CRISPR screens.
DR ChiTaRS; Atg101; mouse.
DR PRO; PR:Q9D8Z6; -.
DR Proteomes; UP000000589; Chromosome 15.
DR RNAct; Q9D8Z6; protein.
DR Bgee; ENSMUSG00000037204; Expressed in granulocyte and 235 other tissues.
DR ExpressionAtlas; Q9D8Z6; baseline and differential.
DR Genevisible; Q9D8Z6; MM.
DR GO; GO:1990316; C:Atg1/ULK1 kinase complex; ISO:MGI.
DR GO; GO:0000407; C:phagophore assembly site; IDA:ComplexPortal.
DR GO; GO:0042802; F:identical protein binding; ISO:MGI.
DR GO; GO:0044877; F:protein-containing complex binding; ISO:MGI.
DR GO; GO:0000045; P:autophagosome assembly; IDA:ComplexPortal.
DR GO; GO:0008285; P:negative regulation of cell population proliferation; IDA:ComplexPortal.
DR GO; GO:0010508; P:positive regulation of autophagy; IDA:ComplexPortal.
DR GO; GO:0046777; P:protein autophosphorylation; IDA:ComplexPortal.
DR GO; GO:0006468; P:protein phosphorylation; IDA:ComplexPortal.
DR GO; GO:1903059; P:regulation of protein lipidation; IDA:ComplexPortal.
DR InterPro; IPR012445; ATG101.
DR PANTHER; PTHR13292; PTHR13292; 1.
DR Pfam; PF07855; ATG101; 1.
PE 1: Evidence at protein level;
KW Autophagy; Cytoplasm; Reference proteome.
FT CHAIN 1..218
FT /note="Autophagy-related protein 101"
FT /id="PRO_0000294323"
FT REGION 152..156
FT /note="Important for interaction with ATG13"
FT /evidence="ECO:0000250|UniProtKB:Q9BSB4"
SQ SEQUENCE 218 AA; 25002 MW; 41E2844EE3EC3884 CRC64;
MNCRSEVLEV SVEGRQVEEA MLAVLHTVLL HRSTGKFHYK KEGTYSIGTV GIQDVDCDFI
DFTYVRVSSE ELDRALRKVV GEFKDALRNS GGDGLGQMSL EFYQKKKSRW PFSDECIPWE
VWTVKVHVVA LATEQERQIC REKVGEKLCE KIINIVEVMS RHEYLPKMPT QSEVDNVFDT
GLRDVQPYLY KISFQITEAL GTSVTTTMRR LIKDTLAL
