ID   ATGSB_MALDO             Reviewed;         455 AA.
AC   V9P9S4;
DT   02-DEC-2020, integrated into UniProtKB/Swiss-Prot.
DT   02-DEC-2020, sequence version 2.
DT   03-AUG-2022, entry version 17.
DE   RecName: Full=Alcohol acyl transferase 1 allele GSb {ECO:0000303|PubMed:24661745};
DE            Short=AAT1-GSb {ECO:0000303|PubMed:24661745};
DE            EC=2.3.1.- {ECO:0000250|UniProtKB:Q64FJ6};
GN   Name=AAT1GSB {ECO:0000303|PubMed:24661745};
OS   Malus domestica (Apple) (Pyrus malus).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Rosales; Rosaceae; Amygdaloideae; Maleae; Malus.
OX   NCBI_TaxID=3750;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-301, TISSUE SPECIFICITY,
RP   DEVELOPMENTAL STAGE, GENE FAMILY, AND NOMENCLATURE.
RC   STRAIN=cv. Granny Smith;
RX   PubMed=24661745; DOI=10.1111/tpj.12518;
RA   Souleyre E.J.F., Chagne D., Chen X., Tomes S., Turner R.M., Wang M.Y.,
RA   Maddumage R., Hunt M.B., Winz R.A., Wiedow C., Hamiaux C., Gardiner S.E.,
RA   Rowan D.D., Atkinson R.G.;
RT   "The AAT1 locus is critical for the biosynthesis of esters contributing to
RT   'ripe apple' flavour in 'Royal Gala' and 'Granny Smith' apples.";
RL   Plant J. 78:903-915(2014).
CC   -!- FUNCTION: Involved in the biosynthesis of volatile esters which confer
CC       ripe apple fruit flavor (By similarity). Alcohol acyl transferase that
CC       can use a wide range of alcohols as substrate to produce esters (By
CC       similarity). {ECO:0000250|UniProtKB:Q64FJ6}.
CC   -!- TISSUE SPECIFICITY: Expressed at very low levels in the skin of ripe
CC       fruit. {ECO:0000269|PubMed:24661745}.
CC   -!- DEVELOPMENTAL STAGE: Accumulates progressively at very low levels
CC       during fruit development, and fades out in ripe fruit.
CC       {ECO:0000269|PubMed:24661745}.
CC   -!- MISCELLANEOUS: The fruit of cv. Royal Gala exhibits a high ester
CC       accumulation, whereas the cv. Granny Smith contains low ester levels;
CC       this influences strongly the ripe apple fruit aroma.
CC       {ECO:0000305|PubMed:24661745}.
CC   -!- SIMILARITY: Belongs to the plant acyltransferase family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AGW30204.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; KC291133; AGW30204.1; ALT_SEQ; Genomic_DNA.
DR   AlphaFoldDB; V9P9S4; -.
DR   SMR; V9P9S4; -.
DR   GO; GO:0016746; F:acyltransferase activity; ISS:UniProtKB.
DR   GO; GO:0006066; P:alcohol metabolic process; ISS:UniProtKB.
DR   GO; GO:0009836; P:fruit ripening, climacteric; IEP:UniProtKB.
DR   Gene3D; 3.30.559.10; -; 2.
DR   InterPro; IPR023213; CAT-like_dom_sf.
PE   2: Evidence at transcript level;
KW   Transferase.
FT   CHAIN           1..455
FT                   /note="Alcohol acyl transferase 1 allele GSb"
FT                   /id="PRO_0000451712"
FT   ACT_SITE        164
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250|UniProtKB:Q9FI78"
FT   ACT_SITE        385
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250|UniProtKB:Q9FI78"
SQ   SEQUENCE   455 AA;  50481 MW;  EB53ACBA4F5C2113 CRC64;
     MMSFSVLQVK RLQLELITPA KPTLQETKFL SDIDDQEGLR FQVPVIMCYK DNPSLNKNCN
     PVKVIREALS RALVYYYPLA GRLKEGPNRK LMVDCNGEGI LFVEASADVT LEQLGDKILP
     PCPLLEEFLF NFPGSDGIIG CPLLLVQVTC LTCGGFILAL RVNHTMCDAP GLLLFLTAIA
     EMARGAHAPS ILPVWERELL FSRDPPRITC VHHEYEDVID HSDGSYASSN QSNMVQRSFY
     FGAKEMRVLR KQIPPHVIST CSTFDLITAC LSKCRTLALK INPKQAVRVS CVVNARGKHH
     NVRLPLGYYG NAFACPAAFS KAEPLCKNPL GYALELVKKA KATMNEEYLR SVADLLVLRG
     RPQYSSTGSY LIVSDNTRAG FGDVNFGWGQ PVFAGPAKAL DLISFYVQHN NNTEDGILVP
     MCLPSSAMER FQQELESITP EPKEDICNNL RSTSQ