ID   ATGSC_MALDO             Reviewed;         456 AA.
AC   A0A498KJ69;
DT   02-DEC-2020, integrated into UniProtKB/Swiss-Prot.
DT   02-DEC-2020, sequence version 2.
DT   03-AUG-2022, entry version 12.
DE   RecName: Full=Alcohol acyl transferase 1 allele GSc {ECO:0000303|PubMed:24661745};
DE            Short=AAT1-GSc {ECO:0000303|PubMed:24661745};
DE            EC=2.3.1.- {ECO:0000250|UniProtKB:Q64FJ6};
GN   Name=AAT1-GSC {ECO:0000303|PubMed:24661745};
GN   ORFNames=DVH24_017674 {ECO:0000312|EMBL:RXI05632.1};
OS   Malus domestica (Apple) (Pyrus malus).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Rosales; Rosaceae; Amygdaloideae; Maleae; Malus.
OX   NCBI_TaxID=3750;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. HFTH1; TISSUE=Leaf;
RA   Hu J.;
RT   "A high-quality apple genome assembly.";
RL   Submitted (OCT-2018) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE,
RP   GENE FAMILY, AND NOMENCLATURE.
RC   STRAIN=cv. Granny Smith;
RX   PubMed=24661745; DOI=10.1111/tpj.12518;
RA   Souleyre E.J.F., Chagne D., Chen X., Tomes S., Turner R.M., Wang M.Y.,
RA   Maddumage R., Hunt M.B., Winz R.A., Wiedow C., Hamiaux C., Gardiner S.E.,
RA   Rowan D.D., Atkinson R.G.;
RT   "The AAT1 locus is critical for the biosynthesis of esters contributing to
RT   'ripe apple' flavour in 'Royal Gala' and 'Granny Smith' apples.";
RL   Plant J. 78:903-915(2014).
CC   -!- FUNCTION: Involved in the biosynthesis of volatile esters which confer
CC       ripe apple fruit flavor (By similarity). Alcohol acyl transferase that
CC       can use a wide range of alcohols as substrate to produce esters (By
CC       similarity). {ECO:0000250|UniProtKB:Q64FJ6}.
CC   -!- TISSUE SPECIFICITY: Expressed at very low levels in the skin of ripe
CC       fruit. {ECO:0000269|PubMed:24661745}.
CC   -!- DEVELOPMENTAL STAGE: Accumulates progressively at very low levels
CC       during fruit development, and fades out in ripe fruit.
CC       {ECO:0000269|PubMed:24661745}.
CC   -!- MISCELLANEOUS: The fruit of cv. Royal Gala exhibits a high ester
CC       accumulation, whereas the cv. Granny Smith contains low ester levels;
CC       this influences strongly the ripe apple fruit aroma.
CC       {ECO:0000305|PubMed:24661745}.
CC   -!- SIMILARITY: Belongs to the plant acyltransferase family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=RXI05632.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; RDQH01000328; RXI05632.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; KC291134; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   AlphaFoldDB; A0A498KJ69; -.
DR   SMR; A0A498KJ69; -.
DR   STRING; 3750.XP_008373477.1; -.
DR   Proteomes; UP000290289; Chromosome 2.
DR   GO; GO:0016746; F:acyltransferase activity; ISS:UniProtKB.
DR   GO; GO:0006066; P:alcohol metabolic process; ISS:UniProtKB.
DR   GO; GO:0009836; P:fruit ripening, climacteric; IEP:UniProtKB.
DR   Gene3D; 3.30.559.10; -; 2.
DR   InterPro; IPR023213; CAT-like_dom_sf.
PE   2: Evidence at transcript level;
KW   Reference proteome; Transferase.
FT   CHAIN           1..456
FT                   /note="Alcohol acyl transferase 1 allele GSc"
FT                   /id="PRO_0000451713"
FT   ACT_SITE        165
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250|UniProtKB:Q9FI78"
FT   ACT_SITE        386
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250|UniProtKB:Q9FI78"
SQ   SEQUENCE   456 AA;  50907 MW;  57B2F64E25A17053 CRC64;
     MMPLAVLQVK RLQPKLITPA KLTPQETKFL SDIDDQEFLR FQVPIIMCYK DNPSLNKNLN
     PVKVIREALS RALVYYYPLA RRLREGPNRK LMVDCNGEGI LFIEASADVT LEQLGDKMLP
     PCPLLEEFLF NFPGSGGIIG CPLVLVQMMT CLTCGGFILA LRLNHTMCDA AGLLLFLTAI
     AEMARGAHAP SILPVWEREL LFAPDPPRIT CAHHEYEDVI GHSDGSYASS NQSNMVQRSF
     YFGAKEMRVL RKQIPPHLTS TCSTFDLITA CLWKYRTLAL NINPKEAVRV SCIVNARGKH
     NNVRLPLGYY GNAFAFPAAI SKAEPLCKNP LGYALELVKK AKATMNEEYL RSVADLLVLR
     GRPQYSSTGS YLIVSDNTRA GFGDVNFGWG QPVFAGPAKA LDLISFYVQH KNNTEDGILV
     PMCLPSSAME RFQQELERIT REPKEDICNN LRSTSQ