ID   AACC8_STRFR             Reviewed;         286 AA.
AC   P29809;
DT   01-APR-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-APR-1993, sequence version 1.
DT   25-MAY-2022, entry version 62.
DE   RecName: Full=Aminoglycoside N(3)-acetyltransferase VIII;
DE            EC=2.3.1.81;
DE   AltName: Full=ACC(3)-VIII;
DE   AltName: Full=Aminocyclitol 3-N-acetyltransferase type VIII;
GN   Name=aacC8;
OS   Streptomyces fradiae (Streptomyces roseoflavus).
OC   Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC   Streptomyces.
OX   NCBI_TaxID=1906;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2060791; DOI=10.1016/0378-1119(91)90237-6;
RA   Salauze D., Perez-Gonzalez J.A., Piepersberg W., Davies J.;
RT   "Characterisation of aminoglycoside acetyltransferase-encoding genes of
RT   neomycin-producing Micromonospora chalcea and Streptomyces fradiae.";
RL   Gene 101:143-148(1991).
CC   -!- FUNCTION: Resistance to neomycin.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2-deoxystreptamine antibiotic + acetyl-CoA = an N(3')-
CC         acetyl-2-deoxystreptamine antibiotic + CoA + H(+);
CC         Xref=Rhea:RHEA:12665, ChEBI:CHEBI:15378, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57288, ChEBI:CHEBI:57921, ChEBI:CHEBI:77452; EC=2.3.1.81;
CC   -!- SIMILARITY: Belongs to the antibiotic N-acetyltransferase family.
CC       {ECO:0000305}.
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DR   EMBL; M55426; AAA26685.1; -; Genomic_DNA.
DR   PIR; JE0417; JE0417.
DR   RefSeq; WP_063856943.1; NG_048574.1.
DR   AlphaFoldDB; P29809; -.
DR   SMR; P29809; -.
DR   KEGG; ag:AAA26685; -.
DR   GO; GO:0046353; F:aminoglycoside 3-N-acetyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   InterPro; IPR003679; Amioglycoside_AcTrfase.
DR   InterPro; IPR028345; Antibiotic_NAT-like.
DR   PANTHER; PTHR11104; PTHR11104; 1.
DR   Pfam; PF02522; Antibiotic_NAT; 1.
DR   SUPFAM; SSF110710; SSF110710; 1.
PE   3: Inferred from homology;
KW   Acyltransferase; Antibiotic resistance; Transferase.
FT   CHAIN           1..286
FT                   /note="Aminoglycoside N(3)-acetyltransferase VIII"
FT                   /id="PRO_0000068550"
SQ   SEQUENCE   286 AA;  30455 MW;  9F5B846BD30D0FA9 CRC64;
     MDEKELIERA GGPVTRGRLV RDLEALGVGA GDTVMVHTRM SAIGYVVGGP QTVIDAVRDA
     VGADGTLMAY CGWNDAPPYD LAEWPPAWRE AARAEWPAYD PLLSEADRGN GRVPEALRHQ
     PGAVRSRHPD ASFVAVGPAA HPLMDDHPWD DPHGPDSPLA RLAGAGGRVL LLGAPLDTLT
     LLHHAEARAE APGKRFVAYE QPVTVGGRRV WRRFRDVDTS RGVPYGRVVP EGVVPFTVIA
     QDMLAAGIGR TGRVAAAPVH LFEAADVVRF GVEWIESRMG GAAGGA