ID   ATGSD_MALDO             Reviewed;         459 AA.
AC   A0A498KIQ3; V9P9R1;
DT   02-DEC-2020, integrated into UniProtKB/Swiss-Prot.
DT   05-JUN-2019, sequence version 1.
DT   03-AUG-2022, entry version 12.
DE   RecName: Full=Alcohol acyl transferase 1 allele GSd {ECO:0000303|PubMed:24661745};
DE            Short=AAT1-GSd {ECO:0000303|PubMed:24661745};
DE            EC=2.3.1.- {ECO:0000250|UniProtKB:Q64FJ6};
GN   Name=AAT1GSD {ECO:0000303|PubMed:24661745};
GN   ORFNames=DVH24_017667 {ECO:0000312|EMBL:RXI05625.1};
OS   Malus domestica (Apple) (Pyrus malus).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Rosales; Rosaceae; Amygdaloideae; Maleae; Malus.
OX   NCBI_TaxID=3750;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE,
RP   GENE FAMILY, AND NOMENCLATURE.
RC   STRAIN=cv. Granny Smith;
RX   PubMed=24661745; DOI=10.1111/tpj.12518;
RA   Souleyre E.J.F., Chagne D., Chen X., Tomes S., Turner R.M., Wang M.Y.,
RA   Maddumage R., Hunt M.B., Winz R.A., Wiedow C., Hamiaux C., Gardiner S.E.,
RA   Rowan D.D., Atkinson R.G.;
RT   "The AAT1 locus is critical for the biosynthesis of esters contributing to
RT   'ripe apple' flavour in 'Royal Gala' and 'Granny Smith' apples.";
RL   Plant J. 78:903-915(2014).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. HFTH1; TISSUE=Leaf;
RA   Hu J.;
RT   "A high-quality apple genome assembly.";
RL   Submitted (OCT-2018) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Involved in the biosynthesis of volatile esters which confer
CC       ripe apple fruit flavor (By similarity). Alcohol acyl transferase that
CC       can use a wide range of alcohols as substrate to produce esters (By
CC       similarity). {ECO:0000250|UniProtKB:Q64FJ6}.
CC   -!- TISSUE SPECIFICITY: Expressed at very low levels in the cortex and skin
CC       of ripe fruit. {ECO:0000269|PubMed:24661745}.
CC   -!- DEVELOPMENTAL STAGE: Accumulates progressively at very low levels
CC       during fruit development, and fades out in ripe fruit.
CC       {ECO:0000269|PubMed:24661745}.
CC   -!- MISCELLANEOUS: The fruit of cv. Royal Gala exhibits a high ester
CC       accumulation, whereas the cv. Granny Smith contains low ester levels;
CC       this influences strongly the ripe apple fruit aroma.
CC       {ECO:0000305|PubMed:24661745}.
CC   -!- SIMILARITY: Belongs to the plant acyltransferase family. {ECO:0000305}.
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DR   EMBL; KC291135; AGW30205.1; -; Genomic_DNA.
DR   EMBL; RDQH01000328; RXI05625.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A498KIQ3; -.
DR   SMR; A0A498KIQ3; -.
DR   STRING; 3750.XP_008384757.1; -.
DR   Proteomes; UP000290289; Chromosome 2.
DR   GO; GO:0016746; F:acyltransferase activity; ISS:UniProtKB.
DR   GO; GO:0006066; P:alcohol metabolic process; ISS:UniProtKB.
DR   GO; GO:0009836; P:fruit ripening, climacteric; IEP:UniProtKB.
DR   Gene3D; 3.30.559.10; -; 2.
DR   InterPro; IPR023213; CAT-like_dom_sf.
PE   2: Evidence at transcript level;
KW   Reference proteome; Transferase.
FT   CHAIN           1..459
FT                   /note="Alcohol acyl transferase 1 allele GSd"
FT                   /id="PRO_0000451714"
FT   ACT_SITE        164
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250|UniProtKB:Q9FI78"
FT   ACT_SITE        385
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250|UniProtKB:Q9FI78"
FT   VARIANT         454..459
FT                   /note="RIMSMM -> SQ (in strain: cv. Granny Smith)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   459 AA;  51235 MW;  38153EA15DCACB90 CRC64;
     MMLPSVLQVK RLQPELITPA KPTPQETKFL SEIDDQESLR FQVPVIMCYK ANPSLNKNRN
     PVKVIREALS RALVYYYPLA GRLREGPNKK LVVDCNGEGI LFVEASADVT LEQLGDKILP
     PCPLLEEFLF NFPGSGGIIG CPLLLVQVTC LTCGGFILAL RLNHTMCDAS GLLLFLTAIA
     EMARGAHAPS ILPVWERELL FARNPPRITC AHHEYEDVID HSDGSYAFSN QSNMVQRSFY
     FGAKEMRVLR KQIPPHLIST CSTFDLITAC LWKCRTLALK INPKQAVRVS CIVNARGKHH
     NVRLPLGYYG NAFAYPAAVS KAEPLCKNPL GYALELVKKA KATMNEEYLR SVADLMVLRG
     RPQYSTTGCY LIVSDNTRAG FGDVNFGWGE PVFAGPAKAL DLISFYVQHK NNTEDGILVP
     MCLSSSAMER FQQELERITQ EPKEDICNNL RSTRIMSMM