PSBI_SPIOL
ID PSBI_SPIOL Reviewed; 36 AA.
AC P62103; P09970;
DT 21-JUN-2004, integrated into UniProtKB/Swiss-Prot.
DT 21-JUN-2004, sequence version 1.
DT 25-MAY-2022, entry version 63.
DE RecName: Full=Photosystem II reaction center protein I {ECO:0000255|HAMAP-Rule:MF_01316};
DE Short=PSII-I {ECO:0000255|HAMAP-Rule:MF_01316};
DE AltName: Full=PSII 4.8 kDa protein {ECO:0000255|HAMAP-Rule:MF_01316, ECO:0000303|PubMed:3058517};
GN Name=psbI {ECO:0000255|HAMAP-Rule:MF_01316};
OS Spinacia oleracea (Spinach).
OG Plastid; Chloroplast.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC Caryophyllales; Chenopodiaceae; Chenopodioideae; Anserineae; Spinacia.
OX NCBI_TaxID=3562;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Geant d'hiver, and cv. Monatol;
RX PubMed=11292076; DOI=10.1023/a:1006478403810;
RA Schmitz-Linneweber C., Maier R.M., Alcaraz J.-P., Cottet A., Herrmann R.G.,
RA Mache R.;
RT "The plastid chromosome of spinach (Spinacia oleracea): complete nucleotide
RT sequence and gene organization.";
RL Plant Mol. Biol. 45:307-315(2001).
RN [2]
RP PROTEIN SEQUENCE OF 1-20, SUBUNIT, AND SUBCELLULAR LOCATION.
RX PubMed=3058517; DOI=10.1016/0014-5793(88)81039-1;
RA Ikeuchi M., Inoue Y.;
RT "A new photosystem II reaction center component (4.8 kDa protein) encoded
RT by chloroplast genome.";
RL FEBS Lett. 241:99-104(1988).
RN [3]
RP PROTEIN SEQUENCE OF 1-20, SUBUNIT, AND SUBCELLULAR LOCATION.
RX PubMed=2644131; DOI=10.1016/0014-5793(89)80482-x;
RA Ikeuchi M., Takio K., Inoue Y.;
RT "N-terminal sequencing of photosystem II low-molecular-mass proteins. 5 and
RT 4.1 kDa components of the O2-evolving core complex from higher plants.";
RL FEBS Lett. 242:263-269(1989).
RN [4]
RP PROTEIN SEQUENCE OF 1-5, SUBUNIT, SUBCELLULAR LOCATION, FORMYLATION AT
RP MET-1, AND MASS SPECTROMETRY.
RX PubMed=9632665; DOI=10.1074/jbc.273.26.16122;
RA Zheleva D., Sharma J., Panico M., Morris H.R., Barber J.;
RT "Isolation and characterization of monomeric and dimeric CP47-reaction
RT center photosystem II complexes.";
RL J. Biol. Chem. 273:16122-16127(1998).
CC -!- FUNCTION: One of the components of the core complex of photosystem II
CC (PSII), required for its stability and/or assembly. PSII is a light-
CC driven water:plastoquinone oxidoreductase that uses light energy to
CC abstract electrons from H(2)O, generating O(2) and a proton gradient
CC subsequently used for ATP formation. It consists of a core antenna
CC complex that captures photons, and an electron transfer chain that
CC converts photonic excitation into a charge separation.
CC {ECO:0000255|HAMAP-Rule:MF_01316}.
CC -!- SUBUNIT: PSII is composed of 1 copy each of membrane proteins PsbA,
CC PsbB, PsbC, PsbD, PsbE, PsbF, PsbH, PsbI, PsbJ, PsbK, PsbL, PsbM, PsbT,
CC PsbX, PsbY, PsbZ, Ycf12, at least 3 peripheral proteins of the oxygen-
CC evolving complex and a large number of cofactors. It forms dimeric
CC complexes. {ECO:0000255|HAMAP-Rule:MF_01316,
CC ECO:0000269|PubMed:2644131, ECO:0000269|PubMed:3058517,
CC ECO:0000269|PubMed:9632665}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane
CC {ECO:0000255|HAMAP-Rule:MF_01316, ECO:0000269|PubMed:2644131,
CC ECO:0000269|PubMed:3058517, ECO:0000269|PubMed:9632665}; Single-pass
CC membrane protein {ECO:0000255|HAMAP-Rule:MF_01316,
CC ECO:0000305|PubMed:2644131, ECO:0000305|PubMed:3058517,
CC ECO:0000305|PubMed:9632665}.
CC -!- MASS SPECTROMETRY: Mass=4195.5; Method=MALDI;
CC Evidence={ECO:0000269|PubMed:9632665};
CC -!- SIMILARITY: Belongs to the PsbI family. {ECO:0000255|HAMAP-
CC Rule:MF_01316}.
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DR EMBL; AJ400848; CAB88709.1; -; Genomic_DNA.
DR PIR; S02002; S02002.
DR RefSeq; NP_054916.1; NC_002202.1.
DR PDB; 3JCU; EM; 3.20 A; I/i=1-36.
DR PDBsum; 3JCU; -.
DR AlphaFoldDB; P62103; -.
DR SMR; P62103; -.
DR DIP; DIP-62015N; -.
DR IntAct; P62103; 1.
DR STRING; 3562.P62103; -.
DR GeneID; 2715614; -.
DR KEGG; soe:2715614; -.
DR OrthoDB; 1641960at2759; -.
DR Proteomes; UP000054095; Chloroplast.
DR GO; GO:0009535; C:chloroplast thylakoid membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0009539; C:photosystem II reaction center; IEA:InterPro.
DR GO; GO:0015979; P:photosynthesis; IEA:UniProtKB-UniRule.
DR HAMAP; MF_01316; PSII_PsbI; 1.
DR InterPro; IPR003686; PSII_PsbI.
DR InterPro; IPR037271; PSII_PsbI_sf.
DR PANTHER; PTHR35772; PTHR35772; 1.
DR Pfam; PF02532; PsbI; 1.
DR SUPFAM; SSF161041; SSF161041; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Chloroplast; Direct protein sequencing; Formylation;
KW Membrane; Photosynthesis; Photosystem II; Plastid; Reaction center;
KW Reference proteome; Thylakoid; Transmembrane; Transmembrane helix.
FT CHAIN 1..36
FT /note="Photosystem II reaction center protein I"
FT /id="PRO_0000219656"
FT TRANSMEM 4..24
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01316"
FT MOD_RES 1
FT /note="N-formylmethionine"
FT /evidence="ECO:0000269|PubMed:9632665"
FT HELIX 2..24
FT /evidence="ECO:0007829|PDB:3JCU"
FT HELIX 27..29
FT /evidence="ECO:0007829|PDB:3JCU"
SQ SEQUENCE 36 AA; 4168 MW; 6B6C7FCB57BB6236 CRC64;
MLTLKLFVYT VVIFFVSLFI FGFLSNDPGR NPGREE
