PSBK_CHLRE
ID PSBK_CHLRE Reviewed; 46 AA.
AC P18263; B7U1E7; Q8HUH4;
DT 01-NOV-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1990, sequence version 1.
DT 03-AUG-2022, entry version 113.
DE RecName: Full=Photosystem II reaction center protein K {ECO:0000255|HAMAP-Rule:MF_00441};
DE Short=PSII-K {ECO:0000255|HAMAP-Rule:MF_00441};
DE Flags: Precursor;
GN Name=psbK {ECO:0000255|HAMAP-Rule:MF_00441};
OS Chlamydomonas reinhardtii (Chlamydomonas smithii).
OG Plastid; Chloroplast.
OC Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Chlorophyceae;
OC CS clade; Chlamydomonadales; Chlamydomonadaceae; Chlamydomonas.
OX NCBI_TaxID=3055;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=137c / CC-125;
RX PubMed=2204032; DOI=10.1093/nar/18.16.4930;
RA Silk G.W., de la Cruz F., Wu M.;
RT "Nucleotide sequence of the chloroplast gene for the 4 kD K polypeptide of
RT photosystem II (psbK) and the psbK-tufA intergenic region of Chlamydomonas
RT reinhardtii.";
RL Nucleic Acids Res. 18:4930-4930(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], IDENTIFICATION, AND COMPLETE
RP PLASTID GENOME.
RX PubMed=12417694; DOI=10.1105/tpc.006155;
RA Maul J.E., Lilly J.W., Cui L., dePamphilis C.W., Miller W., Harris E.H.,
RA Stern D.B.;
RT "The Chlamydomonas reinhardtii plastid chromosome: islands of genes in a
RT sea of repeats.";
RL Plant Cell 14:2659-2679(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CC-503;
RX PubMed=19473533; DOI=10.1186/1471-2148-9-120;
RA Smith D.R., Lee R.W.;
RT "Nucleotide diversity of the Chlamydomonas reinhardtii plastid genome:
RT addressing the mutational-hazard hypothesis.";
RL BMC Evol. Biol. 9:120-120(2009).
RN [4]
RP PROTEIN SEQUENCE OF 10-27, SUBUNIT, AND SUBCELLULAR LOCATION.
RX PubMed=1885590; DOI=10.1016/s0021-9258(18)55345-x;
RA de Vitry C., Diner B.A., Popo J.-L.;
RT "Photosystem II particles from Chlamydomonas reinhardtii. Purification,
RT molecular weight, small subunit composition, and protein phosphorylation.";
RL J. Biol. Chem. 266:16614-16621(1991).
RN [5]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=8193302; DOI=10.1007/bf00029859;
RA Takahashi Y., Matsumoto H., Goldschmidt-Clermont M., Rochaix J.D.;
RT "Directed disruption of the Chlamydomonas chloroplast psbK gene
RT destabilizes the photosystem II reaction center complex.";
RL Plant Mol. Biol. 24:779-788(1994).
RN [6]
RP FUNCTION, SUBUNIT, AND SUBCELLULAR LOCATION.
RC STRAIN=137c / CC-125;
RX PubMed=12939265; DOI=10.1074/jbc.m307537200;
RA Sugimoto I., Takahashi Y.;
RT "Evidence that the PsbK polypeptide is associated with the photosystem II
RT core antenna complex CP43.";
RL J. Biol. Chem. 278:45004-45010(2003).
CC -!- FUNCTION: One of the components of the core complex of photosystem II
CC (PSII). PSII is a light-driven water:plastoquinone oxidoreductase that
CC uses light energy to abstract electrons from H(2)O, generating O(2) and
CC a proton gradient subsequently used for ATP formation. It consists of a
CC core antenna complex that captures photons, and an electron transfer
CC chain that converts photonic excitation into a charge separation.
CC Required for assembly and/or stability of PSII (PubMed:12939265,
CC PubMed:8193302). {ECO:0000255|HAMAP-Rule:MF_00441,
CC ECO:0000269|PubMed:12939265, ECO:0000269|PubMed:8193302}.
CC -!- SUBUNIT: PSII is composed of 1 copy each of membrane proteins PsbA,
CC PsbB, PsbC, PsbD, PsbE, PsbF, PsbH, PsbI, PsbJ, PsbK, PsbL, PsbM, PsbT,
CC PsbX, PsbY, PsbZ, Ycf12, at least 3 peripheral proteins of the oxygen-
CC evolving complex and a large number of cofactors. It forms dimeric
CC complexes. This protein is tightly associated with CP43 (psbC), one of
CC the core proteins (PubMed:12939265). {ECO:0000255|HAMAP-Rule:MF_00441,
CC ECO:0000269|PubMed:12939265, ECO:0000269|PubMed:1885590}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane
CC {ECO:0000255|HAMAP-Rule:MF_00441, ECO:0000269|PubMed:12939265,
CC ECO:0000269|PubMed:1885590}; Single-pass membrane protein
CC {ECO:0000255|HAMAP-Rule:MF_00441, ECO:0000305|PubMed:12939265}.
CC -!- DISRUPTION PHENOTYPE: Not able to grow photoautotrophically, grows
CC normally heterotrophically. Accumulates less than 10% PSII, although
CC the subunits are synthesized normally. {ECO:0000269|PubMed:8193302}.
CC -!- SIMILARITY: Belongs to the PsbK family. {ECO:0000255|HAMAP-
CC Rule:MF_00441}.
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DR EMBL; X53413; CAA37492.1; -; Genomic_DNA.
DR EMBL; AF541861; AAN17817.1; -; Genomic_DNA.
DR EMBL; FJ423446; ACJ50094.1; -; Genomic_DNA.
DR EMBL; BK000554; DAA00907.1; -; Genomic_DNA.
DR PIR; S11162; S11162.
DR RefSeq; NP_958361.1; NC_005353.1.
DR PDB; 6KAC; EM; 2.70 A; K/k=1-46.
DR PDB; 6KAD; EM; 3.40 A; K/k=1-46.
DR PDB; 6KAF; EM; 3.73 A; K/k=1-46.
DR PDBsum; 6KAC; -.
DR PDBsum; 6KAD; -.
DR PDBsum; 6KAF; -.
DR AlphaFoldDB; P18263; -.
DR SMR; P18263; -.
DR STRING; 3055.DAA00907; -.
DR PaxDb; P18263; -.
DR GeneID; 2716978; -.
DR KEGG; cre:ChreCp004; -.
DR eggNOG; ENOG502SDX2; Eukaryota.
DR HOGENOM; CLU_174355_0_0_1; -.
DR InParanoid; P18263; -.
DR OrthoDB; 1631304at2759; -.
DR BioCyc; MetaCyc:CHRECP004-MON; -.
DR Proteomes; UP000006906; Chloroplast.
DR GO; GO:0009535; C:chloroplast thylakoid membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0009539; C:photosystem II reaction center; IEA:InterPro.
DR GO; GO:0015979; P:photosynthesis; IEA:UniProtKB-UniRule.
DR HAMAP; MF_00441; PSII_PsbK; 1.
DR InterPro; IPR003687; PSII_PsbK.
DR InterPro; IPR037270; PSII_PsbK_sf.
DR PANTHER; PTHR35325; PTHR35325; 1.
DR Pfam; PF02533; PsbK; 1.
DR SUPFAM; SSF161037; SSF161037; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Chloroplast; Direct protein sequencing; Membrane;
KW Photosynthesis; Photosystem II; Plastid; Reaction center;
KW Reference proteome; Thylakoid; Transmembrane; Transmembrane helix.
FT PROPEP 1..9
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00441,
FT ECO:0000269|PubMed:1885590"
FT /id="PRO_0000029445"
FT CHAIN 10..46
FT /note="Photosystem II reaction center protein K"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00441"
FT /id="PRO_0000029446"
FT TRANSMEM 18..38
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00441"
FT TURN 13..15
FT /evidence="ECO:0007829|PDB:6KAC"
FT HELIX 16..18
FT /evidence="ECO:0007829|PDB:6KAC"
FT HELIX 19..22
FT /evidence="ECO:0007829|PDB:6KAC"
FT HELIX 25..27
FT /evidence="ECO:0007829|PDB:6KAC"
FT HELIX 28..42
FT /evidence="ECO:0007829|PDB:6KAC"
FT TURN 43..45
FT /evidence="ECO:0007829|PDB:6KAC"
SQ SEQUENCE 46 AA; 5060 MW; 2AB3E5CF8A524F0B CRC64;
MTTLALVLAK LPEAYAPFAP IVDVLPVIPV FFILLAFVWQ AAVSFR
