ATHB6_ARATH
ID ATHB6_ARATH Reviewed; 311 AA.
AC P46668; Q9SJZ1; Q9SQ78;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1995, sequence version 1.
DT 03-AUG-2022, entry version 168.
DE RecName: Full=Homeobox-leucine zipper protein ATHB-6;
DE AltName: Full=HD-ZIP protein ATHB-6;
DE AltName: Full=Homeodomain transcription factor ATHB-6;
GN Name=ATHB-6; OrderedLocusNames=At2g22430; ORFNames=F14M13.17;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=7948864; DOI=10.1007/bf00039527;
RA Soederman E., Mattsson J., Svenson M., Borkird C., Engstroem P.;
RT "Expression patterns of novel genes encoding homeodomain leucine-zipper
RT proteins in Arabidopsis thaliana.";
RL Plant Mol. Biol. 26:145-154(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-291, TISSUE SPECIFICITY, INDUCTION,
RP AND FUNCTION.
RC STRAIN=cv. Columbia;
RX PubMed=10527431; DOI=10.1023/a:1006267013170;
RA Soederman E., Hjellstroem M., Fahleson J., Engstroem P.;
RT "The HD-Zip gene ATHB6 in Arabidopsis is expressed in developing leaves,
RT roots and carpels and up-regulated by water deficit conditions.";
RL Plant Mol. Biol. 40:1073-1083(1999).
RN [6]
RP FUNCTION, MUTAGENESIS OF 1-MET--TYR-43; SER-67 AND 218-LEU--ASN-311,
RP PHOSPHORYLATION, INDUCTION BY ITSELF AND ABA, AND INTERACTION WITH ABI1.
RX PubMed=12065416; DOI=10.1093/emboj/cdf316;
RA Himmelbach A., Hoffmann T., Leube M., Hoehener B., Grill E.;
RT "Homeodomain protein ATHB6 is a target of the protein phosphatase ABI1 and
RT regulates hormone responses in Arabidopsis.";
RL EMBO J. 21:3029-3038(2002).
RN [7]
RP GENE FAMILY, TISSUE SPECIFICITY, AND INDUCTION.
RX PubMed=16055682; DOI=10.1104/pp.105.063461;
RA Henriksson E., Olsson A.S.B., Johannesson H., Johansson H., Hanson J.,
RA Engstroem P., Soederman E.;
RT "Homeodomain leucine zipper class I genes in Arabidopsis. Expression
RT patterns and phylogenetic relationships.";
RL Plant Physiol. 139:509-518(2005).
CC -!- FUNCTION: Transcription activator that may act as growth regulators in
CC response to water deficit. Interacts with the core sequence 5'-
CC CAATTATTA-3' of promoters in response to ABA and in an ABI1-dependent
CC manner. Involved in the negative regulation of the ABA signaling
CC pathway. {ECO:0000269|PubMed:10527431, ECO:0000269|PubMed:12065416}.
CC -!- SUBUNIT: Interacts with ABI1. {ECO:0000269|PubMed:12065416}.
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- TISSUE SPECIFICITY: Widely expressed. {ECO:0000269|PubMed:10527431,
CC ECO:0000269|PubMed:16055682}.
CC -!- INDUCTION: By water deficit, by abscisic acid (ABA) and by salt stress.
CC Self expression regulation. {ECO:0000269|PubMed:10527431,
CC ECO:0000269|PubMed:12065416, ECO:0000269|PubMed:16055682}.
CC -!- PTM: Phosphorylated by PKA. Reversible inactivation of the binding to
CC DNA by phosphorylation. {ECO:0000269|PubMed:12065416}.
CC -!- SIMILARITY: Belongs to the HD-ZIP homeobox family. Class I subfamily.
CC {ECO:0000305}.
CC -!- CAUTION: It is uncertain whether Met-1 or Met-2 is the initiator.
CC {ECO:0000305}.
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DR EMBL; X67034; CAA47427.1; -; mRNA.
DR EMBL; AC006592; AAD22367.2; -; Genomic_DNA.
DR EMBL; CP002685; AEC07305.1; -; Genomic_DNA.
DR EMBL; AY060569; AAL31198.1; -; mRNA.
DR EMBL; AY063819; AAL36175.1; -; mRNA.
DR EMBL; AY094457; AAM19827.1; -; mRNA.
DR EMBL; AY122903; AAM67436.1; -; mRNA.
DR EMBL; AF104900; AAD41726.1; -; Genomic_DNA.
DR PIR; E84612; E84612.
DR PIR; S47136; S47136.
DR RefSeq; NP_565536.1; NM_127808.3.
DR AlphaFoldDB; P46668; -.
DR SMR; P46668; -.
DR BioGRID; 2128; 19.
DR STRING; 3702.AT2G22430.1; -.
DR iPTMnet; P46668; -.
DR PaxDb; P46668; -.
DR PRIDE; P46668; -.
DR ProteomicsDB; 246747; -.
DR EnsemblPlants; AT2G22430.1; AT2G22430.1; AT2G22430.
DR GeneID; 816775; -.
DR Gramene; AT2G22430.1; AT2G22430.1; AT2G22430.
DR KEGG; ath:AT2G22430; -.
DR Araport; AT2G22430; -.
DR TAIR; locus:2041283; AT2G22430.
DR eggNOG; KOG0483; Eukaryota.
DR HOGENOM; CLU_060842_1_0_1; -.
DR InParanoid; P46668; -.
DR OMA; RYGGREF; -.
DR OrthoDB; 1135658at2759; -.
DR PhylomeDB; P46668; -.
DR PRO; PR:P46668; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; P46668; baseline and differential.
DR Genevisible; P46668; AT.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0003677; F:DNA binding; IDA:TAIR.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IEA:InterPro.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:TAIR.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IPI:TAIR.
DR GO; GO:0009738; P:abscisic acid-activated signaling pathway; TAS:TAIR.
DR GO; GO:0009788; P:negative regulation of abscisic acid-activated signaling pathway; IMP:TAIR.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IDA:TAIR.
DR GO; GO:0009414; P:response to water deprivation; IEP:TAIR.
DR CDD; cd00086; homeodomain; 1.
DR InterPro; IPR045224; HDZip_class_I_plant.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR000047; HTH_motif.
DR InterPro; IPR003106; Leu_zip_homeo.
DR PANTHER; PTHR24326; PTHR24326; 1.
DR Pfam; PF02183; HALZ; 1.
DR Pfam; PF00046; Homeodomain; 1.
DR PRINTS; PR00031; HTHREPRESSR.
DR SMART; SM00389; HOX; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
PE 1: Evidence at protein level;
KW Abscisic acid signaling pathway; DNA-binding; Homeobox; Nucleus;
KW Reference proteome; Stress response; Transcription;
KW Transcription regulation.
FT CHAIN 1..311
FT /note="Homeobox-leucine zipper protein ATHB-6"
FT /id="PRO_0000048824"
FT DNA_BIND 59..118
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 1..43
FT /note="Interaction with ABI1"
FT /evidence="ECO:0000269|PubMed:12065416"
FT REGION 1..32
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 119..154
FT /note="Leucine-zipper"
FT REGION 157..183
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 218..311
FT /note="Interaction with ABI1"
FT /evidence="ECO:0000269|PubMed:12065416"
FT COMPBIAS 1..27
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT SITE 67
FT /note="Interaction with ABI1"
FT MUTAGEN 1..43
FT /note="Missing: Reduced interaction with ABI1."
FT /evidence="ECO:0000269|PubMed:12065416"
FT MUTAGEN 67
FT /note="S->A: Reduced interaction with ABI1."
FT /evidence="ECO:0000269|PubMed:12065416"
FT MUTAGEN 218..311
FT /note="Missing: Loss of interaction with ABI1; when
FT associated with 1-M--Y-43 deletion."
FT /evidence="ECO:0000269|PubMed:12065416"
SQ SEQUENCE 311 AA; 35018 MW; 29B19C92A579CF0D CRC64;
MMKRLSSSDS VGGLISLCPT TSTDEQSPRR YGGREFQSML EGYEEEEEAI VEERGHVGLS
EKKRRLSINQ VKALEKNFEL ENKLEPERKV KLAQELGLQP RQVAVWFQNR RARWKTKQLE
KDYGVLKTQY DSLRHNFDSL RRDNESLLQE ISKLKTKLNG GGGEEEEEEN NAAVTTESDI
SVKEEEVSLP EKITEAPSSP PQFLEHSDGL NYRSFTDLRD LLPLKAAASS FAAAAGSSDS
SDSSALLNEE SSSNVTVAAP VTVPGGNFFQ FVKMEQTEDH EDFLSGEEAC EFFSDEQPPS
LHWYSTVDHW N
