AACP1_BRADU
ID AACP1_BRADU Reviewed; 90 AA.
AC Q89VT6;
DT 30-NOV-2010, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 25-MAY-2022, entry version 109.
DE RecName: Full=Aminoacyl carrier protein 1;
GN OrderedLocusNames=bsr0959;
OS Bradyrhizobium diazoefficiens (strain JCM 10833 / BCRC 13528 / IAM 13628 /
OS NBRC 14792 / USDA 110).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC Bradyrhizobiaceae; Bradyrhizobium.
OX NCBI_TaxID=224911;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=JCM 10833 / BCRC 13528 / IAM 13628 / NBRC 14792 / USDA 110;
RX PubMed=12597275; DOI=10.1093/dnares/9.6.189;
RA Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S.,
RA Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M.,
RA Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S.;
RT "Complete genomic sequence of nitrogen-fixing symbiotic bacterium
RT Bradyrhizobium japonicum USDA110.";
RL DNA Res. 9:189-197(2002).
RN [2]
RP FUNCTION AS A CARRIER PROTEIN, PHOSPHOPANTETHEINYLATION, AND IDENTIFICATION
RP BY MASS SPECTROMETRY.
RC STRAIN=JCM 10833 / BCRC 13528 / IAM 13628 / NBRC 14792 / USDA 110;
RX PubMed=20663952; DOI=10.1073/pnas.1007470107;
RA Mocibob M., Ivic N., Bilokapic S., Maier T., Luic M., Ban N.,
RA Weygand-Durasevic I.;
RT "Homologs of aminoacyl-tRNA synthetases acylate carrier proteins and
RT provide a link between ribosomal and nonribosomal peptide synthesis.";
RL Proc. Natl. Acad. Sci. U.S.A. 107:14585-14590(2010).
CC -!- FUNCTION: Aminoacyl carrier protein. Can be charged with L-glycine via
CC the formation of a thioester bond between the amino acid and the 4'-
CC phosphopantetheinyl prosthetic group, catalyzed by the bll0957 ligase.
CC {ECO:0000269|PubMed:20663952}.
CC -!- INTERACTION:
CC Q89VT6; Q89VT8: bll0957; NbExp=4; IntAct=EBI-16043178, EBI-16043152;
CC Q89VT6; Q7CWR3: Atu2573; Xeno; NbExp=2; IntAct=EBI-16043178, EBI-16043272;
CC -!- PTM: 4'-phosphopantetheine is transferred from CoA to a specific serine
CC of the apo-form of this carrier protein. {ECO:0000269|PubMed:20663952}.
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DR EMBL; BA000040; BAC46224.1; -; Genomic_DNA.
DR RefSeq; NP_767599.1; NC_004463.1.
DR RefSeq; WP_011083780.1; NZ_CP011360.1.
DR PDB; 4H2S; X-ray; 2.15 A; C/D=1-90.
DR PDB; 4H2T; X-ray; 2.44 A; C/D=1-90.
DR PDB; 4H2U; X-ray; 2.10 A; C/D=1-90.
DR PDB; 4H2V; X-ray; 2.00 A; C/D=1-90.
DR PDBsum; 4H2S; -.
DR PDBsum; 4H2T; -.
DR PDBsum; 4H2U; -.
DR PDBsum; 4H2V; -.
DR AlphaFoldDB; Q89VT6; -.
DR SMR; Q89VT6; -.
DR DIP; DIP-60152N; -.
DR IntAct; Q89VT6; 2.
DR STRING; 224911.27349209; -.
DR EnsemblBacteria; BAC46224; BAC46224; BAC46224.
DR GeneID; 64020822; -.
DR KEGG; bja:bsr0959; -.
DR PATRIC; fig|224911.44.peg.347; -.
DR eggNOG; COG0236; Bacteria.
DR HOGENOM; CLU_2435073_0_0_5; -.
DR InParanoid; Q89VT6; -.
DR OMA; MEMVNLM; -.
DR PhylomeDB; Q89VT6; -.
DR Proteomes; UP000002526; Chromosome.
DR Gene3D; 1.10.1200.10; -; 1.
DR InterPro; IPR036736; ACP-like_sf.
DR InterPro; IPR009081; PP-bd_ACP.
DR Pfam; PF00550; PP-binding; 1.
DR SUPFAM; SSF47336; SSF47336; 1.
DR PROSITE; PS50075; CARRIER; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Phosphopantetheine; Phosphoprotein; Reference proteome.
FT CHAIN 1..90
FT /note="Aminoacyl carrier protein 1"
FT /id="PRO_0000401189"
FT DOMAIN 6..84
FT /note="Carrier"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
FT MOD_RES 42
FT /note="O-(pantetheine 4'-phosphoryl)serine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
FT HELIX 8..21
FT /evidence="ECO:0007829|PDB:4H2V"
FT TURN 22..24
FT /evidence="ECO:0007829|PDB:4H2U"
FT STRAND 31..33
FT /evidence="ECO:0007829|PDB:4H2S"
FT TURN 35..37
FT /evidence="ECO:0007829|PDB:4H2V"
FT HELIX 42..49
FT /evidence="ECO:0007829|PDB:4H2V"
FT HELIX 67..70
FT /evidence="ECO:0007829|PDB:4H2T"
FT HELIX 73..77
FT /evidence="ECO:0007829|PDB:4H2V"
SQ SEQUENCE 90 AA; 9939 MW; C65EF20A50B7B06E CRC64;
MQAFNTDVRN RIIKLVKGIL EQNALAADVT PQAKLVDVGL TSMDMVNLML GVEAEFDFTI
PQSEITPENF QSVETLERMV MTQLQPATAA
