ATI2_ARATH
ID ATI2_ARATH Reviewed; 266 AA.
AC Q8VY98; B9DH56; Q27GJ8; Q3EAE4;
DT 11-NOV-2015, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 25-MAY-2022, entry version 120.
DE RecName: Full=ATG8-interacting protein 2 {ECO:0000303|PubMed:22253227};
GN Name=ATI2 {ECO:0000303|PubMed:22253227};
GN OrderedLocusNames=At4g00355 {ECO:0000312|Araport:AT4G00355};
GN ORFNames=A_IG005I10.20 {ECO:0000312|EMBL:AAB62839.1},
GN F5I10.20 {ECO:0000312|EMBL:AAF02797.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=cv. Columbia;
RX PubMed=19423640; DOI=10.1093/dnares/dsp009;
RA Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M.,
RA Shinozaki K.;
RT "Analysis of multiple occurrences of alternative splicing events in
RT Arabidopsis thaliana using novel sequenced full-length cDNAs.";
RL DNA Res. 16:155-164(2009).
RN [5]
RP AIM MOTIF.
RX PubMed=20083108; DOI=10.1016/j.febslet.2010.01.018;
RA Noda N.N., Ohsumi Y., Inagaki F.;
RT "Atg8-family interacting motif crucial for selective autophagy.";
RL FEBS Lett. 584:1379-1385(2010).
RN [6]
RP FUNCTION, INTERACTION WITH ATG8F, AND SUBCELLULAR LOCATION.
RX PubMed=22253227; DOI=10.1105/tpc.111.093112;
RA Honig A., Avin-Wittenberg T., Ufaz S., Galili G.;
RT "A new type of compartment, defined by plant-specific Atg8-interacting
RT proteins, is induced upon exposure of Arabidopsis plants to carbon
RT starvation.";
RL Plant Cell 24:288-303(2012).
RN [7]
RP FUNCTION.
RX PubMed=25281689; DOI=10.1105/tpc.114.129999;
RA Michaeli S., Honig A., Levanony H., Peled-Zehavi H., Galili G.;
RT "Arabidopsis ATG8-INTERACTING PROTEIN1 is involved in autophagy-dependent
RT vesicular trafficking of plastid proteins to the vacuole.";
RL Plant Cell 26:4084-4101(2014).
CC -!- FUNCTION: May be involved in salt stress-induced vesicle-to-vacuole
CC trafficking pathway. Through its interaction with ATG8F, may enable
CC delivery of the vesicle bodies to the vacuole by an autophagic pathway
CC (Probable). Plays a role in seed germination in response to exogenous
CC abscisic acid (ABA) treatment (PubMed:22253227).
CC {ECO:0000269|PubMed:22253227, ECO:0000305|PubMed:25281689}.
CC -!- SUBUNIT: Interacts with ATG8F. {ECO:0000269|PubMed:22253227}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000269|PubMed:22253227}; Single-pass membrane protein
CC {ECO:0000255}. Membrane {ECO:0000255}; Single-pass membrane protein
CC {ECO:0000255}. Note=Under favorable growth conditions, partially
CC associated with the endoplasmic reticulum (ER) membrane network. Upon
CC exposure to carbon starvation, mainly associated with newly identified
CC spherical compartments that dynamically move along the ER network and
CC reach the lytic vacuole. {ECO:0000269|PubMed:22253227}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8VY98-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8VY98-2; Sequence=VSP_057970;
CC -!- MISCELLANEOUS: Plants silencing ATI1 and ATI2 display decreased ability
CC of seed germination in presence of exogenous abscisic acid (ABA).
CC {ECO:0000269|PubMed:22253227}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAB62839.1; Type=Erroneous gene model prediction; Note=The predicted gene At4g00350 has been split into 2 genes: At4g00350 and At4g00355.; Evidence={ECO:0000305};
CC Sequence=AAF02797.1; Type=Erroneous gene model prediction; Note=The predicted gene At4g00350 has been split into 2 genes: At4g00350 and At4g00355.; Evidence={ECO:0000305};
CC Sequence=CAB80793.1; Type=Erroneous gene model prediction; Note=The predicted gene At4g00350 has been split into 2 genes: At4g00350 and At4g00355.; Evidence={ECO:0000305};
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DR EMBL; AF013293; AAB62839.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AF195115; AAF02797.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AL161471; CAB80793.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002687; AEE81865.1; -; Genomic_DNA.
DR EMBL; CP002687; AEE81866.1; -; Genomic_DNA.
DR EMBL; CP002687; AEE81867.1; -; Genomic_DNA.
DR EMBL; CP002687; AEE81868.1; -; Genomic_DNA.
DR EMBL; AY072334; AAL61941.1; -; mRNA.
DR EMBL; BT001202; AAN65089.1; -; mRNA.
DR EMBL; AK316778; BAH19497.1; -; mRNA.
DR EMBL; AK317403; BAH20073.1; -; mRNA.
DR EMBL; AK317431; BAH20099.1; -; mRNA.
DR RefSeq; NP_001031565.1; NM_001036488.1. [Q8VY98-2]
DR RefSeq; NP_567174.1; NM_116259.3. [Q8VY98-1]
DR RefSeq; NP_849272.1; NM_178941.4. [Q8VY98-1]
DR RefSeq; NP_849274.2; NM_178943.3. [Q8VY98-1]
DR AlphaFoldDB; Q8VY98; -.
DR STRING; 3702.AT4G00355.3; -.
DR iPTMnet; Q8VY98; -.
DR PaxDb; Q8VY98; -.
DR PRIDE; Q8VY98; -.
DR ProteomicsDB; 246629; -. [Q8VY98-1]
DR EnsemblPlants; AT4G00355.1; AT4G00355.1; AT4G00355. [Q8VY98-1]
DR EnsemblPlants; AT4G00355.2; AT4G00355.2; AT4G00355. [Q8VY98-1]
DR EnsemblPlants; AT4G00355.3; AT4G00355.3; AT4G00355. [Q8VY98-1]
DR EnsemblPlants; AT4G00355.4; AT4G00355.4; AT4G00355. [Q8VY98-2]
DR GeneID; 827659; -.
DR Gramene; AT4G00355.1; AT4G00355.1; AT4G00355. [Q8VY98-1]
DR Gramene; AT4G00355.2; AT4G00355.2; AT4G00355. [Q8VY98-1]
DR Gramene; AT4G00355.3; AT4G00355.3; AT4G00355. [Q8VY98-1]
DR Gramene; AT4G00355.4; AT4G00355.4; AT4G00355. [Q8VY98-2]
DR KEGG; ath:AT4G00355; -.
DR Araport; AT4G00355; -.
DR TAIR; locus:505006416; AT4G00355.
DR eggNOG; KOG1347; Eukaryota.
DR HOGENOM; CLU_054659_0_0_1; -.
DR OMA; RDAEMMM; -.
DR OrthoDB; 1332505at2759; -.
DR PhylomeDB; Q8VY98; -.
DR PRO; PR:Q8VY98; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; Q8VY98; baseline and differential.
DR Genevisible; Q8VY98; AT.
DR GO; GO:0005783; C:endoplasmic reticulum; IDA:TAIR.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0043231; C:intracellular membrane-bounded organelle; IDA:TAIR.
DR GO; GO:0006914; P:autophagy; IEA:UniProtKB-KW.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR InterPro; IPR040304; ATG8-IP-1/2.
DR PANTHER; PTHR34797; PTHR34797; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Autophagy; Endoplasmic reticulum; Membrane;
KW Protein transport; Reference proteome; Stress response; Transmembrane;
KW Transmembrane helix; Transport.
FT CHAIN 1..266
FT /note="ATG8-interacting protein 2"
FT /id="PRO_0000434631"
FT TRANSMEM 191..210
FT /note="Helical"
FT /evidence="ECO:0000255"
FT MOTIF 14..17
FT /note="AIM (Atg8-family-interacting motif)"
FT /evidence="ECO:0000305|PubMed:20083108"
FT MOTIF 218..221
FT /note="AIM (Atg8-family-interacting motif)"
FT /evidence="ECO:0000305|PubMed:20083108"
FT VAR_SEQ 233..266
FT /note="KAGRLMGPISRLKQAFVGGQRRDSFIRASAQNDR -> VFSLGSLFFEPPWY
FT DVSLNYVTTLFGCRKLED (in isoform 2)"
FT /id="VSP_057970"
FT CONFLICT 51
FT /note="E -> G (in Ref. 4; BAH20073)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 266 AA; 29224 MW; E7270806871F6A65 CRC64;
MADKDEAATR GNDWEVVSLT ASAYAAAPGP KPVVDSKDDD HKEVTPCYEA ETSHPLYMSR
HFVFPPTGQL ENTSDLTEAS LTGSHCKEGS DLSLKGLDLS DDFGGLEFSE DKGKKEENIY
TTAMSSLDDE RAIGGSHVYE PVEEPTEPVS PSDVTLDLNP IKDDEVANSP PSEEAWWKRS
VASLIAQAKE TNTVWSICIA AAVMGIVILG QHWQQERWQI LQQKWESSIG NEKAGRLMGP
ISRLKQAFVG GQRRDSFIRA SAQNDR
