PSBL_TOBAC
ID PSBL_TOBAC Reviewed; 38 AA.
AC P60139; O47030; P12166; P12167; Q34007;
DT 28-NOV-2003, integrated into UniProtKB/Swiss-Prot.
DT 28-NOV-2003, sequence version 1.
DT 25-MAY-2022, entry version 61.
DE RecName: Full=Photosystem II reaction center protein L {ECO:0000255|HAMAP-Rule:MF_01317};
DE Short=PSII-L {ECO:0000255|HAMAP-Rule:MF_01317};
GN Name=psbL {ECO:0000255|HAMAP-Rule:MF_01317};
OS Nicotiana tabacum (Common tobacco).
OG Plastid; Chloroplast.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; lamiids; Solanales; Solanaceae; Nicotianoideae; Nicotianeae;
OC Nicotiana.
OX NCBI_TaxID=4097;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Bright Yellow 4;
RX PubMed=16453699; DOI=10.1002/j.1460-2075.1986.tb04464.x;
RA Shinozaki K., Ohme M., Tanaka M., Wakasugi T., Hayashida N.,
RA Matsubayashi T., Zaita N., Chunwongse J., Obokata J.,
RA Yamaguchi-Shinozaki K., Ohto C., Torazawa K., Meng B.-Y., Sugita M.,
RA Deno H., Kamogashira T., Yamada K., Kusuda J., Takaiwa F., Kato A.,
RA Tohdoh N., Shimada H., Sugiura M.;
RT "The complete nucleotide sequence of the tobacco chloroplast genome: its
RT gene organization and expression.";
RL EMBO J. 5:2043-2049(1986).
RN [2]
RP RNA EDITING OF INITIATOR CODON.
RC STRAIN=cv. Havana; TISSUE=Leaf;
RX PubMed=1547774; DOI=10.1002/j.1460-2075.1992.tb05149.x;
RA Kudla J., Igloi G.L., Metzlaff M., Hagemann R., Koessel H.;
RT "RNA editing in tobacco chloroplasts leads to the formation of a
RT translatable psbL mRNA by a C to U substitution within the initiation
RT codon.";
RL EMBO J. 11:1099-1103(1992).
RN [3]
RP RNA EDITING OF INITIATOR CODON.
RC STRAIN=cv. Petit Havana;
RX PubMed=8918473; DOI=10.1002/j.1460-2075.1996.tb00982.x;
RA Chaudhuri S., Maliga P.;
RT "Sequences directing C to U editing of the plastid psbL mRNA are located
RT within a 22 nucleotide segment spanning the editing site.";
RL EMBO J. 15:5958-5964(1996).
RN [4]
RP RNA EDITING OF INITIATOR CODON.
RC STRAIN=cv. Bright Yellow 4; TISSUE=Leaf;
RX PubMed=10589833; DOI=10.1007/s004380051106;
RA Hirose T., Kusumegi T., Tsudzuki T., Sugiura M.;
RT "RNA editing sites in tobacco chloroplast transcripts: editing as a
RT possible regulator of chloroplast RNA polymerase activity.";
RL Mol. Gen. Genet. 262:462-467(1999).
RN [5]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=cv. Petit Havana;
RX PubMed=12655396; DOI=10.1007/s00438-002-0791-1;
RA Swiatek M., Regel R.E., Meurer J., Wanner G., Pakrasi H.B., Ohad I.,
RA Herrmann R.G.;
RT "Effects of selective inactivation of individual genes for low-molecular-
RT mass subunits on the assembly of photosystem II, as revealed by chloroplast
RT transformation: the psbEFLJoperon in Nicotiana tabacum.";
RL Mol. Genet. Genomics 268:699-710(2003).
RN [6]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=cv. Petit Havana;
RX PubMed=14979726; DOI=10.1021/bi0348260;
RA Ohad I., Dal Bosco C., Herrmann R.G., Meurer J.;
RT "Photosystem II proteins PsbL and PsbJ regulate electron flow to the
RT plastoquinone pool.";
RL Biochemistry 43:2297-2308(2004).
CC -!- FUNCTION: Required for PSII activity, may be involved in dimerization
CC as well as contributing to the unidirectionality of electron flow. May
CC prevent reduction of PSII by back electron flow from plastoquinol, thus
CC protecting PSII from photoinactivation (PubMed:14979726).
CC {ECO:0000269|PubMed:12655396, ECO:0000269|PubMed:14979726}.
CC -!- FUNCTION: One of the components of the core complex of photosystem II
CC (PSII). PSII is a light-driven water:plastoquinone oxidoreductase that
CC uses light energy to abstract electrons from H(2)O, generating O(2) and
CC a proton gradient subsequently used for ATP formation. It consists of a
CC core antenna complex that captures photons, and an electron transfer
CC chain that converts photonic excitation into a charge separation. This
CC subunit is found at the monomer-monomer interface and is required for
CC correct PSII assembly and/or dimerization. {ECO:0000255|HAMAP-
CC Rule:MF_01317}.
CC -!- SUBUNIT: PSII is composed of 1 copy each of membrane proteins PsbA,
CC PsbB, PsbC, PsbD, PsbE, PsbF, PsbH, PsbI, PsbJ, PsbK, PsbL, PsbM, PsbT,
CC PsbX, PsbY, PsbZ, Ycf12, at least 3 peripheral proteins of the oxygen-
CC evolving complex and a large number of cofactors. It forms dimeric
CC complexes. {ECO:0000255|HAMAP-Rule:MF_01317}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane
CC {ECO:0000255|HAMAP-Rule:MF_01317}; Single-pass membrane protein
CC {ECO:0000255|HAMAP-Rule:MF_01317}.
CC -!- RNA EDITING: Modified_positions=1 {ECO:0000269|PubMed:10589833,
CC ECO:0000269|PubMed:1547774, ECO:0000269|PubMed:8918473}; Note=The
CC initiator methionine is created by RNA editing.;
CC -!- DISRUPTION PHENOTYPE: Plants unable to grow photoautotrophically,
CC although under low light (10 umol photons/m(2)/s) on a carbon source
CC plants are green. Young leaves have residual PSII activity, PSII
CC present mostly as monomers rather then dimers. Highly photosensitive.
CC {ECO:0000269|PubMed:12655396}.
CC -!- SIMILARITY: Belongs to the PsbL family. {ECO:0000255|HAMAP-
CC Rule:MF_01317}.
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DR EMBL; Z00044; CAA77418.1; ALT_SEQ; Genomic_DNA.
DR PIR; S05685; S05685.
DR RefSeq; NP_054515.2; NC_001879.2.
DR AlphaFoldDB; P60139; -.
DR SMR; P60139; -.
DR GeneID; 800491; -.
DR KEGG; nta:800491; -.
DR OrthoDB; 1638606at2759; -.
DR GO; GO:0009535; C:chloroplast thylakoid membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0009539; C:photosystem II reaction center; IEA:InterPro.
DR GO; GO:0015979; P:photosynthesis; IEA:UniProtKB-UniRule.
DR HAMAP; MF_01317; PSII_PsbL; 1.
DR InterPro; IPR003372; PSII_PsbL.
DR InterPro; IPR037266; PSII_PsbL_sf.
DR Pfam; PF02419; PsbL; 1.
DR SUPFAM; SSF161017; SSF161017; 1.
PE 2: Evidence at transcript level;
KW Chloroplast; Membrane; Photosynthesis; Photosystem II; Plastid;
KW Reaction center; RNA editing; Thylakoid; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..38
FT /note="Photosystem II reaction center protein L"
FT /id="PRO_0000219778"
FT TRANSMEM 17..37
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01317"
SQ SEQUENCE 38 AA; 4497 MW; 55537AEC50D25E8D CRC64;
MTQSNPNEQN VELNRTSLYW GLLLIFVLAV LFSNYFFN
