ID   ATIF_YEAST              Reviewed;          85 AA.
AC   P01097; D6VRH1;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1990, sequence version 2.
DT   03-AUG-2022, entry version 181.
DE   RecName: Full=ATPase inhibitor, mitochondrial {ECO:0000305};
DE   AltName: Full=ATP synthase F1 subunit epsilon {ECO:0000250|UniProtKB:Q9UII2};
DE   Flags: Precursor;
GN   Name=INH1; OrderedLocusNames=YDL181W; ORFNames=D1305;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2138617; DOI=10.1016/s0021-9258(19)39321-4;
RA   Ichikawa N., Yoshida Y., Hashimoto T., Ogasawara N., Yoshikawa H.,
RA   Imamoto F., Tagawa K.;
RT   "Activation of ATP hydrolysis by an uncoupler in mutant mitochondria
RT   lacking an intrinsic ATPase inhibitor in yeast.";
RL   J. Biol. Chem. 265:6274-6278(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 96604 / S288c / FY1679;
RX   PubMed=8533471; DOI=10.1002/yea.320111007;
RA   Verhasselt P., Voet M., Volckaert G.;
RT   "New open reading frames, one of which is similar to the nifV gene of
RT   Azotobacter vinelandii, found on a 12.5 kbp fragment of chromosome IV of
RT   Saccharomyces cerevisiae.";
RL   Yeast 11:961-966(1995).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=9169867;
RA   Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G.,
RA   Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C.,
RA   Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F.,
RA   Delaveau T., del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M.,
RA   Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T.,
RA   Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C.,
RA   Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S.,
RA   Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L.,
RA   Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H.,
RA   Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M.,
RA   Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M.,
RA   Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A.,
RA   Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G.,
RA   Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E.,
RA   Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S.,
RA   Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D.,
RA   Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V.,
RA   Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E.,
RA   Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M.,
RA   Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D.,
RA   Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X.,
RA   Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A.,
RA   Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R.,
RA   Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T.,
RA   Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L.,
RA   Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E.,
RA   Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L.,
RA   Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M.,
RA   Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K.,
RA   Mewes H.-W., Zollner A., Zaccaria P.;
RT   "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.";
RL   Nature 387:75-78(1997).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=17322287; DOI=10.1101/gr.6037607;
RA   Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA   Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA   Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA   Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA   LaBaer J.;
RT   "Approaching a complete repository of sequence-verified protein-encoding
RT   clones for Saccharomyces cerevisiae.";
RL   Genome Res. 17:536-543(2007).
RN   [6]
RP   PROTEIN SEQUENCE OF 23-85.
RX   PubMed=6458601; DOI=10.1093/oxfordjournals.jbchem.a133568;
RA   Matsubara H., Hase T., Hashimoto T., Tagawa K.;
RT   "Amino acid sequence of an intrinsic inhibitor of mitochondrial ATPase from
RT   yeast.";
RL   J. Biochem. 90:1159-1165(1981).
RN   [7]
RP   PROTEIN SEQUENCE OF 1-28.
RX   PubMed=6619114; DOI=10.1093/oxfordjournals.jbchem.a134342;
RA   Yoshida Y., Hashimoto T., Hase T., Matsubara H., Tagawa K.;
RT   "Partial amino terminal sequence of the precursor of mitochondrial ATPase
RT   inhibitor protein synthesized with mRNA partially purified by gel
RT   permeation chromatography.";
RL   J. Biochem. 94:291-297(1983).
RN   [8]
RP   MUTAGENESIS OF LYS-41.
RX   PubMed=10705452; DOI=10.1271/bbb.64.89;
RA   Ichikawa N., Fujisaka R., Kuribayashi R.;
RT   "Requirement for lysine-19 of the yeast mitochondrial ATPase inhibitor for
RT   the stability of the inactivated inhibitor-F1Fo complex at higher pH.";
RL   Biosci. Biotechnol. Biochem. 64:89-95(2000).
RN   [9]
RP   LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX   PubMed=14562106; DOI=10.1038/nature02046;
RA   Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA   O'Shea E.K., Weissman J.S.;
RT   "Global analysis of protein expression in yeast.";
RL   Nature 425:737-741(2003).
CC   -!- FUNCTION: Forms a one-to-one complex with ATPase to inhibit the enzyme
CC       activity completely.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion.
CC   -!- MISCELLANEOUS: Present with 981 molecules/cell in log phase SD medium.
CC       {ECO:0000269|PubMed:14562106}.
CC   -!- SIMILARITY: Belongs to the ATPase inhibitor family. {ECO:0000305}.
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DR   EMBL; D00443; BAA00344.1; -; Genomic_DNA.
DR   EMBL; X83276; CAA58265.1; -; Genomic_DNA.
DR   EMBL; Z74229; CAA98755.1; -; Genomic_DNA.
DR   EMBL; AY558374; AAS56700.1; -; Genomic_DNA.
DR   EMBL; BK006938; DAA11681.1; -; Genomic_DNA.
DR   PIR; A35231; IWBY.
DR   RefSeq; NP_010100.1; NM_001180241.1.
DR   PDB; 3ZIA; X-ray; 2.50 A; J/T=23-85.
DR   PDBsum; 3ZIA; -.
DR   AlphaFoldDB; P01097; -.
DR   SMR; P01097; -.
DR   BioGRID; 31863; 100.
DR   DIP; DIP-2985N; -.
DR   IntAct; P01097; 7.
DR   MINT; P01097; -.
DR   STRING; 4932.YDL181W; -.
DR   MaxQB; P01097; -.
DR   PaxDb; P01097; -.
DR   PRIDE; P01097; -.
DR   EnsemblFungi; YDL181W_mRNA; YDL181W; YDL181W.
DR   GeneID; 851347; -.
DR   KEGG; sce:YDL181W; -.
DR   SGD; S000002340; INH1.
DR   VEuPathDB; FungiDB:YDL181W; -.
DR   eggNOG; ENOG502SCJG; Eukaryota.
DR   GeneTree; ENSGT00940000176616; -.
DR   HOGENOM; CLU_145563_4_0_1; -.
DR   InParanoid; P01097; -.
DR   OMA; DAFTRRE; -.
DR   BioCyc; YEAST:G3O-29568-MON; -.
DR   PRO; PR:P01097; -.
DR   Proteomes; UP000002311; Chromosome IV.
DR   RNAct; P01097; protein.
DR   GO; GO:0005739; C:mitochondrion; IDA:SGD.
DR   GO; GO:0042030; F:ATPase inhibitor activity; IDA:SGD.
DR   GO; GO:0004857; F:enzyme inhibitor activity; IDA:SGD.
DR   GO; GO:0032780; P:negative regulation of ATP-dependent activity; IDA:SGD.
DR   DisProt; DP00995; -.
DR   InterPro; IPR007648; ATPase_inhibitor_mt.
DR   Pfam; PF04568; IATP; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Coiled coil; Direct protein sequencing; Mitochondrion;
KW   Reference proteome; Transit peptide.
FT   TRANSIT         1..22
FT                   /note="Mitochondrion"
FT                   /evidence="ECO:0000269|PubMed:2138617,
FT                   ECO:0000269|PubMed:6458601"
FT   CHAIN           23..85
FT                   /note="ATPase inhibitor, mitochondrial"
FT                   /id="PRO_0000002554"
FT   COILED          41..84
FT                   /evidence="ECO:0000255"
FT   MUTAGEN         41
FT                   /note="K->Q,E: No loss of inhibitory activity."
FT                   /evidence="ECO:0000269|PubMed:10705452"
FT   TURN            24..28
FT                   /evidence="ECO:0007829|PDB:3ZIA"
FT   HELIX           38..57
FT                   /evidence="ECO:0007829|PDB:3ZIA"
SQ   SEQUENCE   85 AA;  9870 MW;  E1AF73A47F63CE58 CRC64;
     MLPRSALARS LQLQRGVAAR FYSEGSTGTP RGSGSEDSFV KRERATEDFF VRQREKEQLR
     HLKEQLEKQR KKIDSLENKI DSMTK