ID   ATI_COWPX               Reviewed;        1284 AA.
AC   P16602;
DT   01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1990, sequence version 1.
DT   03-AUG-2022, entry version 55.
DE   RecName: Full=A-type inclusion protein A25 homolog;
DE            Short=ATI;
GN   Name=ATI;
OS   Cowpox virus (CPV).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus.
OX   NCBI_TaxID=10243;
OH   NCBI_TaxID=10129; Apodemus sylvaticus (European woodmouse).
OH   NCBI_TaxID=9913; Bos taurus (Bovine).
OH   NCBI_TaxID=9685; Felis catus (Cat) (Felis silvestris catus).
OH   NCBI_TaxID=9606; Homo sapiens (Human).
OH   NCBI_TaxID=9785; Loxodonta africana (African elephant).
OH   NCBI_TaxID=29092; Microtus agrestis (Short-tailed field vole).
OH   NCBI_TaxID=10090; Mus musculus (Mouse).
OH   NCBI_TaxID=447135; Myodes glareolus (Bank vole) (Clethrionomys glareolus).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=CPRO6;
RX   PubMed=2826668; DOI=10.1099/0022-1317-69-1-35;
RA   Funahashi S., Sato T., Shida H.;
RT   "Cloning and characterization of the gene encoding the major protein of the
RT   A-type inclusion body of cowpox virus.";
RL   J. Gen. Virol. 69:35-47(1988).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-109.
RX   PubMed=2828037; DOI=10.1002/j.1460-2075.1987.tb02714.x;
RA   Patel D.D., Pickup D.J.;
RT   "Messenger RNAs of a strongly-expressed late gene of cowpox virus contain
RT   5'-terminal poly(A) sequences.";
RL   EMBO J. 6:3787-3794(1987).
CC   -!- FUNCTION: Structural protein that forms a matrix surrounding the mature
CC       virion (MV) through interaction with protein A26. Presence of protein
CC       A25 in the virion structurally prevents direct virus-cell fusion
CC       mechanism (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Interacts (via N-terminus) with protein A26. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Virion. Note=Present above the membrane of mature
CC       virions (MV). {ECO:0000250}.
CC   -!- MISCELLANEOUS: Some orthopoxviruses such as cowpox, ectromelia, and
CC       raccoonpox viruses, form large cytoplasmic inclusions within which
CC       mature virions are embedded by a process called occlusion. In those
CC       viruses, A26 bridges mature virion with inclusion bodies through
CC       interactions with proteins A25 and A27 on the mature virion membrane.
CC   -!- SIMILARITY: Belongs to the poxviridae A25 protein family.
CC       {ECO:0000305}.
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DR   EMBL; D00319; BAA00222.1; -; Genomic_DNA.
DR   EMBL; X06343; CAA29650.1; -; Genomic_DNA.
DR   PIR; JQ0006; WMVZAI.
DR   SMR; P16602; -.
DR   PRIDE; P16602; -.
DR   GO; GO:0016032; P:viral process; IEA:InterPro.
DR   InterPro; IPR007596; Pox_A_type_inc.
DR   Pfam; PF04508; Pox_A_type_inc; 10.
PE   3: Inferred from homology;
KW   Late protein; Repeat; Virion.
FT   CHAIN           1..1284
FT                   /note="A-type inclusion protein A25 homolog"
FT                   /id="PRO_0000099284"
FT   REPEAT          611..637
FT                   /note="1"
FT   REPEAT          638..665
FT                   /note="2"
FT   REPEAT          666..689
FT                   /note="3"
FT   REPEAT          690..720
FT                   /note="4"
FT   REPEAT          721..751
FT                   /note="5"
FT   REPEAT          752..780
FT                   /note="6"
FT   REPEAT          781..811
FT                   /note="7"
FT   REPEAT          812..842
FT                   /note="8"
FT   REPEAT          843..871
FT                   /note="9"
FT   REPEAT          872..912
FT                   /note="10"
FT   REGION          340..383
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          611..912
FT                   /note="10 X approximate tandem repeats"
FT   REGION          1169..1234
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1209..1223
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   1284 AA;  150330 MW;  F7904C9E1DE8D012 CRC64;
     MEVTNLIEKC TKHSKDFATE VEKLWNDELS SESGLSRKTR NVIRNILRDI TKSLTTDKKS
     KCFRILERST INGEQIKDVY KTIFNNGVDV ESRINTTGKY VLFTVMTYAA AELRLIKSDE
     IFALLSRFFN MICDIHRKYG CGNMFVGIPA ALINLLEIDQ INKLFSVFST RYDAKTFIYT
     EYFLFLNINH YLLVSGSELF INVAYGPASF SSPISVPDYI MEALTFKACD HIMKSGDLKY
     TYAFTKKVKD LFNTKSDSVY QYVRLHEMSY DGVSEDTDDD DEVFAILNLS IDSSVDRYRN
     RVLLLTPEVA SLRKEYSDVE PDYKYLMDEE VPAYDKHLPK PITNTGIEEP HATGGDKEEQ
     EQQPVKVVQS KPDDGITPYN PFEDPDYVPT ITKTVLGIAD YQLVINKLIE WLDKCEEECG
     NGGEYKTELE EAKRKLTELN LELSDKLSKI RTLERDSVYK TERIDRLTKE IKELRDIQNG
     TDDGSDSSEI DKKTIRELRE SLDREREMRT ELERELDTIR DGKVEGSCQR ELELSRMWLK
     QRDDDLRAEI DKRRNVEWEL SRLRRDIKEC DKYKEDLDKA KTTISNYVSR ISTLESEIAK
     YQQDRDTLSV VRRELEEERR RVRDLESRLD ECTRNQEDTQ EVDALRSRIR ELENKLADCM
     ESGGGNLTEI SRLQSKISDL ERQLRECRGN ATEISRLQYR ITDLERQLND CRRNNENNAD
     TEREMQRLRD RITDLERQLS DCRRNNESNA DMEREMQRLR DRIMDLDRQL NECKRDGNGT
     SSEEVNRLKT RIRDLERSLE ICSKDESELY SAYKSELGRA REQISNLQES LRRERESDKT
     DSYYRRELTR ERNKIVELEK ELNKCFDTNH AKYIDEINSK KTRISDLERQ LAACKSNGGS
     NGDMDQYKRE IESLKRELAE CRRGNNGSHS DCKYYDEEAR EEVKRLRQEL TQLHEDLKRA
     RESDKNDSYY KRELERQRAK VIEVEKELER YFDDSRLAEC KRHGDEMLRK IADLEKKLRD
     GGNGNGGNGC TSSCEFERKR IAVLEVEVRK SMETIKSLEK FMEFDRLQKD CADKLDREKE
     RRMKAERDLE REIARKNCGG NPCERELESE RSNVKRLEYQ LDAEKEKVKF YKRELERDRY
     LSSRYLTSSS DPDEKPLPNY TFPRIEVEPL TTEDTEPKPV EVVPPSSDVT EPISSGVTPS
     VDAEPEHPQL SEYQTSVSQV AVTPPPKPET PQISEYQDYS ELYSASNNTE SKNVFSELAY
     LDDLDKLDDI DEYLLNNIMP EKTV