ATI_VACCW
ID ATI_VACCW Reviewed; 725 AA.
AC P24759; Q80HU8;
DT 01-MAR-1992, integrated into UniProtKB/Swiss-Prot.
DT 27-JUL-2011, sequence version 2.
DT 25-MAY-2022, entry version 73.
DE RecName: Full=A-type inclusion protein A25;
DE Short=ATI;
GN OrderedLocusNames=VACWR148; ORFNames=A25;
OS Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain
OS WR)).
OC Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus; Vaccinia virus.
OX NCBI_TaxID=10254;
OH NCBI_TaxID=9913; Bos taurus (Bovine).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=1856205; DOI=10.1016/s0021-9258(18)92757-2;
RA Amegadzie B.Y., Ahn B.-Y., Moss B.;
RT "Identification, sequence, and expression of the gene encoding a Mr 35,000
RT subunit of the vaccinia virus DNA-dependent RNA polymerase.";
RL J. Biol. Chem. 266:13712-13718(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=1733111; DOI=10.1016/0042-6822(92)90046-r;
RA Amegadzie B.Y., Sisler J.R., Moss B.;
RT "Frame-shift mutations within the vaccinia virus A-type inclusion protein
RT gene.";
RL Virology 186:777-782(1992).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=1962448; DOI=10.1016/0042-6822(91)90548-p;
RA de Carlos A., Paez E.;
RT "Isolation and characterization of mutants of vaccinia virus with a
RT modified 94-kDa inclusion protein.";
RL Virology 185:768-778(1991).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Esposito J.J., Frace A.M., Sammons S.A., Olsen-Rasmussen M., Osborne J.,
RA Wohlhueter R.;
RT "Sequencing of the coding region of Vaccinia-WR to an average 9-fold
RT redundancy and an error rate of 0.16/10kb.";
RL Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP INTERACTION WITH PROTEIN A26.
RX PubMed=20484506; DOI=10.1128/jvi.00704-10;
RA Howard A.R., Weisberg A.S., Moss B.;
RT "Congregation of orthopoxvirus virions in cytoplasmic A-type inclusions is
RT mediated by interactions of a bridging protein (A26p) with a matrix protein
RT (ATIp) and a virion membrane-associated protein (A27p).";
RL J. Virol. 84:7592-7602(2010).
RN [6]
RP FUNCTION.
RX PubMed=20538855; DOI=10.1128/jvi.00599-10;
RA Chang S.J., Chang Y.X., Izmailyan R., Tang Y.L., Chang W.;
RT "Vaccinia virus A25 and A26 proteins are fusion suppressors for mature
RT virions and determine strain-specific virus entry pathways into HeLa, CHO-
RT K1, and L cells.";
RL J. Virol. 84:8422-8432(2010).
CC -!- FUNCTION: Structural protein that forms a matrix surrounding the mature
CC virion (MV) through interaction with protein A26. Presence of protein
CC A25 in the virion structurally prevents direct virus-cell fusion
CC mechanism. {ECO:0000269|PubMed:20538855}.
CC -!- SUBUNIT: Interacts (via N-terminus) with protein A26.
CC {ECO:0000269|PubMed:20484506}.
CC -!- SUBCELLULAR LOCATION: Virion. Note=Present above the membrane of mature
CC virions (MV).
CC -!- MISCELLANEOUS: Some orthopoxviruses such as cowpox, ectromelia, and
CC raccoonpox viruses, form large cytoplasmic inclusions within which
CC mature virions are embedded by a process called occlusion. In those
CC viruses, A26 bridges mature virion with inclusion bodies through
CC interactions with proteins A25 and A27 on the mature virion membrane.
CC In vaccinia virus, the protein A25 is deleted in its C-terminal region
CC and no inclusion body is observed.
CC -!- SIMILARITY: Belongs to the poxviridae A25 protein family.
CC {ECO:0000305}.
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DR EMBL; M61187; AAA48321.1; -; Genomic_DNA.
DR EMBL; M76371; AAA48275.1; -; Genomic_DNA.
DR EMBL; X57318; CAA40574.1; -; Genomic_DNA.
DR EMBL; AY243312; AAO89427.1; -; Genomic_DNA.
DR PIR; A41701; WMVZ94.
DR RefSeq; YP_233030.1; NC_006998.1.
DR SMR; P24759; -.
DR IntAct; P24759; 1.
DR MINT; P24759; -.
DR DNASU; 3707678; -.
DR GeneID; 3707678; -.
DR KEGG; vg:3707678; -.
DR Proteomes; UP000000344; Genome.
DR GO; GO:0016032; P:viral process; IEA:InterPro.
DR InterPro; IPR007596; Pox_A_type_inc.
DR Pfam; PF04508; Pox_A_type_inc; 4.
PE 1: Evidence at protein level;
KW Coiled coil; Reference proteome; Repeat; Virion.
FT CHAIN 1..725
FT /note="A-type inclusion protein A25"
FT /id="PRO_0000099279"
FT REPEAT 609..635
FT /note="1"
FT REPEAT 636..663
FT /note="2"
FT REPEAT 664..687
FT /note="3"
FT REPEAT 699..725
FT /note="4"
FT REGION 609..718
FT /note="4 X approximate tandem repeats"
FT COILED 418..521
FT /evidence="ECO:0000255"
FT COILED 547..719
FT /evidence="ECO:0000255"
FT CONFLICT 587..588
FT /note="SK -> KQ (in Ref. 1; AAA48321 and 3; CAA40574)"
FT /evidence="ECO:0000305"
FT CONFLICT 610
FT /note="R -> H (in Ref. 2; AAA48275)"
FT /evidence="ECO:0000305"
FT CONFLICT 619
FT /note="Missing (in Ref. 1; AAA48321 and 3; CAA40574)"
FT /evidence="ECO:0000305"
FT CONFLICT 683
FT /note="S -> R (in Ref. 2; AAA48275)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 725 AA; 84351 MW; 6763AADA13E7AAAA CRC64;
MEVTNLIEKC TKHSKDFATE VKKLWNDELS SESGLSRKTR NVIRNILRDI TKSLTTDKKS
KCFRILERST INGEQIKDVY KTIFNNGVDV ESRINTTGKY VLFTVMTYVA AELRLIKSDE
IFALLSRFFN MICDIHRKYG CGNMFVGIPA ALIILLEIDH INKLFSVFST RYDAKAYLYT
EYFLFLNINH YLLSGSDLFI NVAYGAVSFS SPISVPDYIM EALTFKACDH IMKSGDLKYT
YAFTKKVKDL FNTKSDSIYQ YVRLHEMSYD GVSEDTDDDD EVFAILNLSI DSSVDRYRNR
VLLLTPEVAS LRKEYSDVEP DYKYLMDEEV PAYDKHLPKP ITNTGIEEPH ATGGDEDQPI
KVVHPPNNDK DDAIKPYNPL EDPNYVPTIT RTAIGIADYQ LVINKLIEWL DKCEEECGNS
GEFKTELEEA KRKLTELNAE LSDKLSKIRT LERDSVYKTE RIDRLTKEIK EHRDIQNGTD
DGSDLLEIDK KTIRELRESL DREREMRSEL EKELDTIRNG KVDGSCQREL ELSRMWLKQR
DDDLRAEIDK RRNVEWELSR LRRDIKECDK YKEDLDKAKT TISNYVSKIS TLESEIAKYQ
QDRDTLSVVR RELEEERRRV RDLESRLDEC TRNQEDTQEV DALRSRIREL ENKLTDCIES
GGGNLTEISR LQSKISDLER QLSECRENAT EISRLQSRIS DLERQLNDCR RNNETNAETE
RDATS
