ID   ATI_VAR67               Reviewed;         702 AA.
AC   P34011;
DT   01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1994, sequence version 1.
DT   29-SEP-2021, entry version 63.
DE   RecName: Full=A-type inclusion protein A25 homolog;
DE            Short=ATI;
GN   ORFNames=A28L;
OS   Variola virus (isolate Human/India/Ind3/1967) (VARV) (Smallpox virus).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus.
OX   NCBI_TaxID=587200;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1666548;
RA   Shchelkunov S.N., Marennikova S.S., Totmenin A.V., Blinov V.M.,
RA   Chizhikov V.E., Gutorov V.V., Safronov P.F., Pozdnyakov S.G.,
RA   Shelukhina E.M., Gashnikov P.V., Anjaparidze O.G., Sandakhchiev L.S.;
RT   "Creation of a clone library of fragments from the natural variola virus
RT   and study of the structural and functional organization of viral genes from
RT   a circle of hosts.";
RL   Dokl. Akad. Nauk SSSR 321:402-406(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8384129; DOI=10.1016/0014-5793(93)80041-r;
RA   Shchelkunov S.N., Blinov V.M., Sandakhchiev L.S.;
RT   "Genes of variola and vaccinia viruses necessary to overcome the host
RT   protective mechanisms.";
RL   FEBS Lett. 319:80-83(1993).
CC   -!- FUNCTION: Structural protein that forms a matrix surrounding the mature
CC       virion (MV) through interaction with protein A26. Presence of protein
CC       A25 in the virion structurally prevents direct virus-cell fusion
CC       mechanism (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Interacts (via N-terminus) with protein A26. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Virion. Note=Present above the membrane of mature
CC       virions (MV). {ECO:0000250}.
CC   -!- MISCELLANEOUS: Some orthopoxviruses such as cowpox, ectromelia, and
CC       raccoonpox viruses, form large cytoplasmic inclusions within which
CC       mature virions are embedded by a process called occlusion. In those
CC       viruses, A26 bridges mature virion with inclusion bodies through
CC       interactions with proteins A25 and A27 on the mature virion membrane.
CC       In variola virus, the protein A25 is deleted in its C-terminal region
CC       and no inclusion body is observed.
CC   -!- SIMILARITY: Belongs to the poxviridae A25 protein family.
CC       {ECO:0000305}.
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DR   EMBL; X69198; CAA49073.1; -; Genomic_DNA.
DR   EMBL; X67115; CAA47498.1; -; Genomic_DNA.
DR   PIR; S46854; S46854.
DR   RefSeq; NP_042176.1; NC_001611.1.
DR   SMR; P34011; -.
DR   PRIDE; P34011; -.
DR   GeneID; 1486504; -.
DR   KEGG; vg:1486504; -.
DR   Proteomes; UP000002060; Genome.
DR   GO; GO:0016032; P:viral process; IEA:InterPro.
DR   InterPro; IPR007596; Pox_A_type_inc.
DR   Pfam; PF04508; Pox_A_type_inc; 3.
PE   3: Inferred from homology;
KW   Coiled coil; Reference proteome; Virion.
FT   CHAIN           1..702
FT                   /note="A-type inclusion protein A25 homolog"
FT                   /id="PRO_0000099280"
FT   REGION          342..380
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          420..522
FT                   /evidence="ECO:0000255"
FT   COILED          548..692
FT                   /evidence="ECO:0000255"
FT   COMPBIAS        350..378
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   702 AA;  81284 MW;  74FBA1EBF921E3B3 CRC64;
     MEVTNLIEKC TKHSKDFATE VEKLWNDELS SESCLSRKTR NVIRNILRDI TKSLTTDKKS
     KCFRILERST INGEQIKDVY KTIFNNGVDA ESRINTTGKY VLFTVMTYAA AELRLIKSDE
     IFALLSRFFN MICDIHKKYG CGNMFVGIPA ALIVLLEIDH INKLFSVFST RYDAKAYLYT
     EYFLFLNINH YLLSGSDLFI NVAYGAVSFS SPISVPDYIM EALTFKACDH IMKSGDLIYI
     YAFTKKVKDL FNTKSDSIYQ YVRLHEMSYD GVSEDTDDDD EVFAILNLSI DSSVDRYRNR
     VLLLTPEVAS LRKEYSEAEP DYKYLMDEEV PAYDKHLPKP ITNTGIEEPH ATGGDKEDQP
     IKVVHPPNND KDDAIKSYNP LEDPNYVPTI TRTAIGIADY QLVINKLIEW LDKCEEECGN
     GGEFKTELEE AKRKLTELNA ELSDKLSKIR TLERDSVYKT ERIDRLTKEI KELRDIQNGT
     DDGSDSSEID KKTIRELKES LDREREMRSE LEKELDTIRD GKVDGSCQGR LELSRMWLKQ
     RDDDLRAEID KRRNVEWELS KLRRDIKECD KYKEELDKAK TTISNYVSRI STLESEIAKY
     QQDRDTLSAV RGELEEERRR VRDLESRLDE CTHNQKDTQE VDALRSRISE LENKLTDCIE
     SGGGNLTEIS RLQSRILDLE RQLNDCRHNN KTNTETDRNE TS