ATK_DROME
ID ATK_DROME Reviewed; 1535 AA.
AC Q9VPF0; E3CTU4; F0JAL6; Q6NR19;
DT 07-SEP-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 2.
DT 03-AUG-2022, entry version 161.
DE RecName: Full=Protein artichoke {ECO:0000312|FlyBase:FBgn0036995};
DE Flags: Precursor;
GN Name=atk {ECO:0000312|FlyBase:FBgn0036995};
GN ORFNames=CG5195 {ECO:0000312|FlyBase:FBgn0036995};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley {ECO:0000312|EMBL:AAQ23582.1};
RC TISSUE=Embryo {ECO:0000312|EMBL:AAQ23582.1};
RA Stapleton M., Booth B., Brokstein P., Hong L., Agbayani A., Carlson J.,
RA Champe M., Chavez C., Dorsett V., Dresnek D., Farfan D., Frise E.,
RA George R., Gonzalez M., Guarin H., Kronmiller B., Li P., Liao G.,
RA Miranda A., Mungall C.J., Nunoo J., Pacleb J., Paragas V., Park S.,
RA Patel S., Phouanenavong S., Wan K., Yu C., Lewis S.E., Rubin G.M.,
RA Celniker S.;
RL Submitted (FEB-2011) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=24496014; DOI=10.1534/genetics.113.156323;
RA Andres M., Turiegano E., Goepfert M.C., Canal I., Torroja L.;
RT "The extracellular matrix protein artichoke is required for integrity of
RT ciliated mechanosensory and chemosensory organs in Drosophila embryos.";
RL Genetics 196:1091-1102(2014).
CC -!- FUNCTION: Required for normal morphology and function of ciliated
CC sensory organs. {ECO:0000269|PubMed:24496014}.
CC -!- SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular
CC matrix {ECO:0000269|PubMed:24496014}. Cytoplasm
CC {ECO:0000269|PubMed:24496014}.
CC -!- DEVELOPMENTAL STAGE: During embryonic stage 16-17, expressed in
CC accessory cells of all ciliated mechanosensory and chemosensory organs
CC (at protein level). Specifically, detected in the cap cell of
CC chordotonal organs and the shaft cell of external sensory organs (at
CC protein level). Also detected in chemosensory organs including the
CC pharyngeal organs, dorsal organ, terminal organ and ventral organ (at
CC protein level). Expression appears to be temporally restricted to late
CC embryogenesis and is not detected during larval stages (at protein
CC level). {ECO:0000269|PubMed:24496014}.
CC -!- DISRUPTION PHENOTYPE: Viable and fertile. Larval locomotion is severely
CC uncoordinated and the chemotactic response to sucrose is reduced.
CC Morphology of the lch5 mechanosensory organ is abnormal, with
CC disorganized cilia and neuronal cell bodies, although cilia still make
CC contact with the cap cell. {ECO:0000269|PubMed:24496014}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAQ23582.1; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=ADP89557.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AE014296; AAF51605.2; -; Genomic_DNA.
DR EMBL; AE014296; AGB94806.1; -; Genomic_DNA.
DR EMBL; AE014296; AGB94807.1; -; Genomic_DNA.
DR EMBL; BT010264; AAQ23582.1; ALT_FRAME; mRNA.
DR EMBL; BT125760; ADP89557.1; ALT_FRAME; mRNA.
DR EMBL; BT126058; ADY17757.1; -; mRNA.
DR RefSeq; NP_001262113.1; NM_001275184.1.
DR RefSeq; NP_001262114.1; NM_001275185.1.
DR RefSeq; NP_649228.1; NM_140971.1.
DR AlphaFoldDB; Q9VPF0; -.
DR SMR; Q9VPF0; -.
DR PaxDb; Q9VPF0; -.
DR EnsemblMetazoa; FBtr0078223; FBpp0077881; FBgn0036995.
DR EnsemblMetazoa; FBtr0331569; FBpp0303959; FBgn0036995.
DR EnsemblMetazoa; FBtr0331570; FBpp0303960; FBgn0036995.
DR GeneID; 40266; -.
DR KEGG; dme:Dmel_CG5195; -.
DR UCSC; CG5195-RA; d. melanogaster.
DR CTD; 40266; -.
DR FlyBase; FBgn0036995; atk.
DR VEuPathDB; VectorBase:FBgn0036995; -.
DR eggNOG; KOG0619; Eukaryota.
DR HOGENOM; CLU_003890_0_0_1; -.
DR InParanoid; Q9VPF0; -.
DR OMA; AILPCRC; -.
DR OrthoDB; 826997at2759; -.
DR PhylomeDB; Q9VPF0; -.
DR BioGRID-ORCS; 40266; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 40266; -.
DR PRO; PR:Q9VPF0; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0036995; Expressed in chemosensory organ and 9 other tissues.
DR GO; GO:0005737; C:cytoplasm; IDA:FlyBase.
DR GO; GO:0031012; C:extracellular matrix; IDA:FlyBase.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR GO; GO:0060271; P:cilium assembly; IMP:FlyBase.
DR GO; GO:0040011; P:locomotion; IMP:FlyBase.
DR GO; GO:0050896; P:response to stimulus; IEA:UniProtKB-KW.
DR GO; GO:0007423; P:sensory organ development; IMP:FlyBase.
DR Gene3D; 3.80.10.10; -; 6.
DR InterPro; IPR000483; Cys-rich_flank_reg_C.
DR InterPro; IPR001611; Leu-rich_rpt.
DR InterPro; IPR003591; Leu-rich_rpt_typical-subtyp.
DR InterPro; IPR032675; LRR_dom_sf.
DR Pfam; PF13855; LRR_8; 7.
DR SMART; SM00369; LRR_TYP; 28.
DR SMART; SM00082; LRRCT; 1.
DR PROSITE; PS51450; LRR; 29.
PE 1: Evidence at protein level;
KW Cytoplasm; Extracellular matrix; Leucine-rich repeat; Reference proteome;
KW Repeat; Secreted; Sensory transduction; Signal.
FT SIGNAL 1..19
FT /evidence="ECO:0000255"
FT CHAIN 20..1535
FT /note="Protein artichoke"
FT /evidence="ECO:0000255"
FT /id="PRO_5007325100"
FT REPEAT 64..87
FT /note="LRR 1"
FT /evidence="ECO:0000255"
FT REPEAT 89..110
FT /note="LRR 2"
FT /evidence="ECO:0000255"
FT REPEAT 112..135
FT /note="LRR 3"
FT /evidence="ECO:0000255"
FT REPEAT 136..157
FT /note="LRR 4"
FT /evidence="ECO:0000255"
FT REPEAT 158..181
FT /note="LRR 5"
FT /evidence="ECO:0000255"
FT REPEAT 183..206
FT /note="LRR 6"
FT /evidence="ECO:0000255"
FT REPEAT 207..230
FT /note="LRR 7"
FT /evidence="ECO:0000255"
FT REPEAT 231..256
FT /note="LRR 8"
FT /evidence="ECO:0000255"
FT REPEAT 257..280
FT /note="LRR 9"
FT /evidence="ECO:0000255"
FT REPEAT 281..304
FT /note="LRR 10"
FT /evidence="ECO:0000255"
FT REPEAT 306..328
FT /note="LRR 11"
FT /evidence="ECO:0000255"
FT REPEAT 331..356
FT /note="LRR 12"
FT /evidence="ECO:0000255"
FT REPEAT 357..380
FT /note="LRR 13"
FT /evidence="ECO:0000255"
FT REPEAT 382..404
FT /note="LRR 14"
FT /evidence="ECO:0000255"
FT REPEAT 406..429
FT /note="LRR 15"
FT /evidence="ECO:0000255"
FT REPEAT 430..452
FT /note="LRR 16"
FT /evidence="ECO:0000255"
FT REPEAT 453..476
FT /note="LRR 17"
FT /evidence="ECO:0000255"
FT REPEAT 478..500
FT /note="LRR 18"
FT /evidence="ECO:0000255"
FT REPEAT 521..545
FT /note="LRR 19"
FT /evidence="ECO:0000255"
FT REPEAT 548..571
FT /note="LRR 20"
FT /evidence="ECO:0000255"
FT REPEAT 573..595
FT /note="LRR 21"
FT /evidence="ECO:0000255"
FT REPEAT 597..619
FT /note="LRR 22"
FT /evidence="ECO:0000255"
FT REPEAT 620..643
FT /note="LRR 23"
FT /evidence="ECO:0000255"
FT REPEAT 645..667
FT /note="LRR 24"
FT /evidence="ECO:0000255"
FT REPEAT 669..691
FT /note="LRR 25"
FT /evidence="ECO:0000255"
FT REPEAT 692..714
FT /note="LRR 26"
FT /evidence="ECO:0000255"
FT REPEAT 716..738
FT /note="LRR 27"
FT /evidence="ECO:0000255"
FT REPEAT 739..762
FT /note="LRR 28"
FT /evidence="ECO:0000255"
FT REPEAT 764..786
FT /note="LRR 29"
FT /evidence="ECO:0000255"
FT REPEAT 788..810
FT /note="LRR 30"
FT /evidence="ECO:0000255"
FT REPEAT 811..834
FT /note="LRR 31"
FT /evidence="ECO:0000255"
FT REPEAT 835..858
FT /note="LRR 32"
FT /evidence="ECO:0000255"
FT REPEAT 860..882
FT /note="LRR 33"
FT /evidence="ECO:0000255"
FT REPEAT 883..906
FT /note="LRR 34"
FT /evidence="ECO:0000255"
FT DOMAIN 919..963
FT /note="LRRCT"
FT /evidence="ECO:0000255"
FT REGION 1036..1055
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1253..1331
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1377..1416
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1429..1449
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1255..1327
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1377..1395
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 500
FT /note="H -> R (in Ref. 3; AAQ23582/ADP89557)"
FT /evidence="ECO:0000305"
FT CONFLICT 1268
FT /note="E -> K (in Ref. 3; AAQ23582/ADP89557)"
FT /evidence="ECO:0000305"
FT CONFLICT 1434
FT /note="E -> G (in Ref. 3; AAQ23582/ADP89557)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1535 AA; 172130 MW; BAEC078AB2D59C17 CRC64;
MMLLPIFLLL CIGINLIRAE SCPPSQAILP CRCSLRGKEI QIWCSHSNLP QIMDGLKAVE
RNIKGRIDEL VLENNQLPAL PGRFFGSLQI VRLMLRHNSI ERVSNGWLNE LENGLVEIFV
VEPQLRSIPA ESLNGMINML AITIQSEELK HLPDFSGLLS LTYLSVQTGA LQELPSHLFR
HLPKLQHIHI TGGSGLTRLE AGLFDGLISL KNLDLSHNGL NWIHLRALSR LPNLVSLKLS
HNQISDVGMV GRIVKDLEHL KKLRLDNNLI TVIEDGSFVD LPNLSELHLN DNRITELQYG
AFLRTPQLKT IYLQNNLIRR IHPESLLQAS GSGVEAVHMY NNEIGHVEAL RALLDALPRL
RYLDMSGNLL SELPYGALRG HGTLEQLHLN HNHLRLIERD ALMAMPALRE LRMRNNSLSS
DLPLPFWNLP GLKGLDLAQN QFARVDSQLL AGLPSLRRLD LSENGLIELA PNSFRHNPLL
ETLNISSNEL TKIHSSTLIH LERLFEVDAS YNQLKSVIAG LPRIVERISL KGNQITSLPA
AASKDLQLPN LRMLDLSQNR IEQLPRHGFQ GAMELRVLSL AQNELRQLKD TSFIGIQRLE
LLHLQENQLG EADERALLPL AELRNLNLQS NKLEAITDNF FSNNSRLEQL DLSRNLIRSI
SPTAFDTQRS LEYLDLSGNA LLDISVGLGN LNNLRDIDLS YNQISRIQSD VIGGWRNVVE
IRLSNNLIVE LQQGTFRNLP KLQYLDLSSN EIRNVEPGAL KGLDELQEFV LADNKLVELK
DHVFEELPSL LASHFQYNKL RYISPESFHN ANSLVFLNLS NNHFRNMENI GLRSMRNLEV
LDLSTNGVKL VSTMPLKALN WLVELKMDNN QICRIQGSPF ETMPRLRVLS MRNNQLRSIK
ERTFRNVRGN IAILDVDGNP IDCNCEMQWL SVWLQETNFP YPGPKCQDGR LLRSARMERS
LCVGADIYGN ERTDGNQLPL LNEHGDVFQR DLPDDFNDEC EAGEGTRLPG DRPLVGESEY
FYDQYVDATE APDTTHSAIS TSQRPKPTPT INSNIDLNNT ILHTKYFNRK PQPGSGSPFT
FFGYPLPSVS LGRFFGFGDR GRKQRTDSND DMPATHRMAH ISLPSGRGKT RMYQPNSAEF
EKYLKDQQKQ EKLNIARNRY VDTDSTTSSM EDALSNESGS AATTVGVFRT TFREPSSIER
GGFRPIVPAH VGGFMPVHDP QQRRGLVEVV NITGNPSEIV QGIGQRKFIP ISTQARPKPT
KSSGESSETA TYEVTETTPD ITTTTPLMQR IATSTTKAST TTTRSTTTTS TTQVTPAENN
ASSSTELDSQ YDDEDLEAQS VTLLRPPPLV QTTTAETILL IPPAEEHVAQ IKSRSWVTTT
TPQSPSDNQV TLAGPTSTVP PPPPASPPLR GGGRSTITKV YTPYQQQVAQ PTAEEYQRTT
PSGDNEGVAS EHQLTANAQK RTELELLQVD RVDRKDGMDW YYESFKKKRD FNGGAAVRKT
AHKEVFYDGV AASSSWNRLH KEILFISLLL LWRAC
