PSBO2_ARATH
ID PSBO2_ARATH Reviewed; 331 AA.
AC Q9S841;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 135.
DE RecName: Full=Oxygen-evolving enhancer protein 1-2, chloroplastic;
DE Short=OEE1;
DE AltName: Full=33 kDa subunit of oxygen evolving system of photosystem II;
DE AltName: Full=33 kDa thylakoid membrane protein;
DE AltName: Full=Manganese-stabilizing protein 2;
DE Short=MSP-2;
DE AltName: Full=OEC 33 kDa subunit;
DE Flags: Precursor;
GN Name=PSBO2; OrderedLocusNames=At3g50820; ORFNames=F18B3.100;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Columbia;
RA Legen J., Misera S., Herrmann R.G., Altschmied L.;
RT "Sequences and map position of 31 Arabidopsis thaliana cDNAs encoding
RT organellar polypeptides.";
RL Submitted (AUG-1999) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP PROTEIN SEQUENCE OF 85-92, AND SUBCELLULAR LOCATION.
RX PubMed=11719511; DOI=10.1074/jbc.m108575200;
RA Schubert M., Petersson U.A., Haas B.J., Funk C., Schroeder W.P.,
RA Kieselbach T.;
RT "Proteome map of the chloroplast lumen of Arabidopsis thaliana.";
RL J. Biol. Chem. 277:8354-8365(2002).
RN [7]
RP PROTEIN SEQUENCE OF N-TERMINUS, AND IDENTIFICATION BY MASS SPECTROMETRY.
RX PubMed=11826309; DOI=10.1105/tpc.010304;
RA Peltier J.-B., Emanuelsson O., Kalume D.E., Ytterberg J., Friso G.,
RA Rudella A., Liberles D.A., Soederberg L., Roepstorff P., von Heijne G.,
RA van Wijk K.J.;
RT "Central functions of the lumenal and peripheral thylakoid proteome of
RT Arabidopsis determined by experimentation and genome-wide prediction.";
RL Plant Cell 14:211-236(2002).
RN [8]
RP FUNCTION.
RX PubMed=15722336; DOI=10.1074/jbc.m501550200;
RA Yi X., McChargue M., Laborde S., Frankel L.K., Bricker T.M.;
RT "The manganese-stabilizing protein is required for photosystem II
RT assembly/stability and photoautotrophy in higher plants.";
RL J. Biol. Chem. 280:16170-16174(2005).
RN [9]
RP FUNCTION.
RX PubMed=17217465; DOI=10.1111/j.1365-313x.2006.02976.x;
RA Lundin B., Hansson M., Schoefs B., Vener A.V., Spetea C.;
RT "The Arabidopsis PsbO2 protein regulates dephosphorylation and turnover of
RT the photosystem II reaction centre D1 protein.";
RL Plant J. 49:528-539(2007).
RN [10]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND SUBCELLULAR LOCATION [LARGE SCALE
RP ANALYSIS].
RX PubMed=18431481; DOI=10.1371/journal.pone.0001994;
RA Zybailov B., Rutschow H., Friso G., Rudella A., Emanuelsson O., Sun Q.,
RA van Wijk K.J.;
RT "Sorting signals, N-terminal modifications and abundance of the chloroplast
RT proteome.";
RL PLoS ONE 3:E1994-E1994(2008).
RN [11]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22092075; DOI=10.1021/pr200917t;
RA Aryal U.K., Krochko J.E., Ross A.R.;
RT "Identification of phosphoproteins in Arabidopsis thaliana leaves using
RT polyethylene glycol fractionation, immobilized metal-ion affinity
RT chromatography, two-dimensional gel electrophoresis and mass
RT spectrometry.";
RL J. Proteome Res. 11:425-437(2012).
CC -!- FUNCTION: Stabilizes the manganese cluster which is the primary site of
CC water splitting. Regulates dephosphorylation and turnover of the PSII
CC reaction center D1 protein. {ECO:0000269|PubMed:15722336,
CC ECO:0000269|PubMed:17217465}.
CC -!- INTERACTION:
CC Q9S841; P0AA25: trxA; Xeno; NbExp=2; IntAct=EBI-449424, EBI-368542;
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane
CC {ECO:0000269|PubMed:11719511, ECO:0000269|PubMed:18431481}; Peripheral
CC membrane protein {ECO:0000269|PubMed:11719511}; Lumenal side
CC {ECO:0000269|PubMed:11719511}. Note=Associated with the photosystem II
CC complex.
CC -!- SIMILARITY: Belongs to the PsbO family. {ECO:0000305}.
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DR EMBL; AJ145957; CAB53092.1; -; mRNA.
DR EMBL; AL049862; CAB42911.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE78714.1; -; Genomic_DNA.
DR EMBL; AY050362; AAK91379.1; -; mRNA.
DR EMBL; AY116934; AAM51568.1; -; mRNA.
DR EMBL; AY088799; AAM67110.1; -; mRNA.
DR PIR; T08403; T08403.
DR RefSeq; NP_190651.1; NM_114942.3.
DR AlphaFoldDB; Q9S841; -.
DR SMR; Q9S841; -.
DR BioGRID; 9564; 3.
DR IntAct; Q9S841; 3.
DR STRING; 3702.AT3G50820.1; -.
DR TCDB; 3.E.2.2.3; the photosynthetic reaction center (prc) family.
DR iPTMnet; Q9S841; -.
DR MetOSite; Q9S841; -.
DR SWISS-2DPAGE; Q9S841; -.
DR PaxDb; Q9S841; -.
DR PRIDE; Q9S841; -.
DR ProteomicsDB; 225996; -.
DR DNASU; 824246; -.
DR EnsemblPlants; AT3G50820.1; AT3G50820.1; AT3G50820.
DR GeneID; 824246; -.
DR Gramene; AT3G50820.1; AT3G50820.1; AT3G50820.
DR KEGG; ath:AT3G50820; -.
DR Araport; AT3G50820; -.
DR TAIR; locus:2077972; AT3G50820.
DR eggNOG; ENOG502QRXA; Eukaryota.
DR HOGENOM; CLU_063138_0_0_1; -.
DR InParanoid; Q9S841; -.
DR OMA; GKANDCP; -.
DR OrthoDB; 924219at2759; -.
DR PhylomeDB; Q9S841; -.
DR BioCyc; ARA:AT3G50820-MON; -.
DR PRO; PR:Q9S841; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9S841; baseline and differential.
DR Genevisible; Q9S841; AT.
DR GO; GO:0009507; C:chloroplast; HDA:TAIR.
DR GO; GO:0009570; C:chloroplast stroma; HDA:TAIR.
DR GO; GO:0009534; C:chloroplast thylakoid; HDA:TAIR.
DR GO; GO:0009535; C:chloroplast thylakoid membrane; HDA:TAIR.
DR GO; GO:0005829; C:cytosol; HDA:TAIR.
DR GO; GO:0009654; C:photosystem II oxygen evolving complex; IEA:InterPro.
DR GO; GO:0010287; C:plastoglobule; HDA:TAIR.
DR GO; GO:0009579; C:thylakoid; IDA:TAIR.
DR GO; GO:0031977; C:thylakoid lumen; HDA:TAIR.
DR GO; GO:0003729; F:mRNA binding; IDA:TAIR.
DR GO; GO:0010242; F:oxygen evolving activity; IEA:InterPro.
DR GO; GO:0008266; F:poly(U) RNA binding; IDA:TAIR.
DR GO; GO:0010205; P:photoinhibition; IMP:TAIR.
DR GO; GO:0019684; P:photosynthesis, light reaction; ISS:TAIR.
DR GO; GO:0010207; P:photosystem II assembly; IMP:TAIR.
DR GO; GO:0042549; P:photosystem II stabilization; IMP:TAIR.
DR GO; GO:0035304; P:regulation of protein dephosphorylation; IMP:TAIR.
DR InterPro; IPR011250; OMP/PagP_b-brl.
DR InterPro; IPR002628; PSII_MSP.
DR PANTHER; PTHR34058; PTHR34058; 1.
DR Pfam; PF01716; MSP; 1.
DR SUPFAM; SSF56925; SSF56925; 1.
PE 1: Evidence at protein level;
KW Chloroplast; Direct protein sequencing; Manganese; Membrane;
KW Photosynthesis; Photosystem II; Plastid; Reference proteome; Thylakoid;
KW Transit peptide.
FT TRANSIT 1..57
FT /note="Chloroplast"
FT /evidence="ECO:0000250"
FT TRANSIT 58..84
FT /note="Thylakoid"
FT /evidence="ECO:0000269|PubMed:11719511,
FT ECO:0000269|PubMed:11826309"
FT CHAIN 85..331
FT /note="Oxygen-evolving enhancer protein 1-2, chloroplastic"
FT /id="PRO_0000029554"
SQ SEQUENCE 331 AA; 35019 MW; 08F837E651B1C2E7 CRC64;
MATSLQAAAT FLQPAKIAAS PSRNVHLRSN QTVGKSFGLD SSQARLTCSL HSDLKDFAGK
CSDAAKIAGF ALATSALVVS GAGAEGAPKR LTYDEIQSKT YMEVKGTGTA NQCPTIDGGS
ETFSFKAGKY TGKKFCFEPT SFTVKADSVS KNAPPDFQNT KLMTRLTYTL DEIEGPFEVG
SDGSVKFKEE DGIDYAAVTV QLPGGERVPF LFTVKQLEAS GKPESFSGKF LVPSYRGSSF
LDPKGRGGST GYDNAVALPA GGRGDEEELS KENVKNTAAS VGEITLKITK SKPETGEVIG
VFESLQPSDT DLGAKVPKDV KIQGVWYGQI E
