ID   PSBO_CHAMQ              Reviewed;         318 AA.
AC   A0A4Y1YTM1; C0HLQ8;
DT   17-JUN-2020, integrated into UniProtKB/Swiss-Prot.
DT   18-SEP-2019, sequence version 1.
DT   25-MAY-2022, entry version 11.
DE   RecName: Full=Oxygen-evolving enhancer protein 1, chloroplastic {ECO:0000305};
DE            Short=OEE1 {ECO:0000305};
DE   Flags: Precursor;
OS   Chattonella marina var. antiqua (Red tide flagellate) (Chattonella
OS   antiqua).
OC   Eukaryota; Sar; Stramenopiles; Ochrophyta; Raphidophyceae; Chattonellales;
OC   Chattonellaceae; Chattonella.
OX   NCBI_TaxID=859642;
RN   [1] {ECO:0000312|EMBL:BBL54382.1}
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=NIES-1 {ECO:0000312|EMBL:BBL54382.1};
RA   Mukai K., Qiu X., Shimasaki Y., Oshima Y.;
RT   "Protein identified by two-dimensional electrophoresis in Chattonella
RT   marina var. antiqua.";
RL   Submitted (JUN-2019) to the EMBL/GenBank/DDBJ databases.
RN   [2] {ECO:0000305}
RP   PROTEIN SEQUENCE OF 65-84, AND INDUCTION.
RC   STRAIN=NIES-1 {ECO:0000303|PubMed:23291769};
RX   PubMed=23291769; DOI=10.1271/bbb.120543;
RA   Qiu X., Shimasaki Y., Tsuyama M., Yamada T., Kuwahara R., Kawaguchi M.,
RA   Honda M., Gunjikake H., Tasmin R., Shimizu M., Sato Y., Kato-Unoki Y.,
RA   Nakashima T., Matsubara T., Yamasaki Y., Ichinose H., Wariishi H.,
RA   Honjo T., Oshima Y.;
RT   "Growth-phase dependent variation in photosynthetic activity and cellular
RT   protein expression profile in the harmful raphidophyte Chattonella
RT   antiqua.";
RL   Biosci. Biotechnol. Biochem. 77:46-52(2013).
RN   [3] {ECO:0000305}
RP   PROTEIN SEQUENCE OF 65-84, AND INDUCTION.
RC   STRAIN=NIES-1 {ECO:0000303|Ref.3};
RX   DOI=10.1016/j.jembe.2020.151361;
RA   Qiu X., Mukai K., Shimasaki Y., Wu M., Chen C., Lu Y., Ichinose H.,
RA   Nakashima T., Kato-Unoki Y., Oshima Y.;
RT   "Diurnal variations in expression of photosynthesis-related proteins in the
RT   harmful Raphidophyceae Chattonella marina var. antiqua.";
RL   J. Exp. Mar. Biol. Ecol. 527:0-0(2020).
CC   -!- FUNCTION: Stabilizes the manganese cluster which is the primary site of
CC       water splitting. {ECO:0000250|UniProtKB:P23321}.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane
CC       {ECO:0000250|UniProtKB:P23321}; Single-pass membrane protein
CC       {ECO:0000255}. Note=Associated with the photosystem II complex.
CC       {ECO:0000250|UniProtKB:P23321}.
CC   -!- INDUCTION: Expression shows a diurnal pattern of oscillation across the
CC       24-hour light-dark, with increased levels during the light period (at
CC       protein level) (Ref.3). Down-regulated in the late stationary growth
CC       phase as compared to the early stationary and exponential growth phases
CC       (PubMed:23291769). {ECO:0000269|PubMed:23291769, ECO:0000269|Ref.3}.
CC   -!- SIMILARITY: Belongs to the PsbO family. {ECO:0000305}.
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DR   EMBL; LC486728; BBL54382.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A4Y1YTM1; -.
DR   SMR; A0A4Y1YTM1; -.
DR   GO; GO:0009535; C:chloroplast thylakoid membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0009654; C:photosystem II oxygen evolving complex; IEA:InterPro.
DR   GO; GO:0010207; P:photosystem II assembly; IEA:InterPro.
DR   GO; GO:0042549; P:photosystem II stabilization; IEA:InterPro.
DR   InterPro; IPR011250; OMP/PagP_b-brl.
DR   InterPro; IPR002628; PSII_MSP.
DR   PANTHER; PTHR34058; PTHR34058; 1.
DR   Pfam; PF01716; MSP; 1.
DR   SUPFAM; SSF56925; SSF56925; 1.
PE   1: Evidence at protein level;
KW   Chloroplast; Direct protein sequencing; Membrane; Photosynthesis;
KW   Photosystem II; Plastid; Signal; Thylakoid; Transmembrane;
KW   Transmembrane helix.
FT   SIGNAL          1..18
FT                   /evidence="ECO:0000255"
FT   CHAIN           19..318
FT                   /note="Oxygen-evolving enhancer protein 1, chloroplastic"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_5021476367"
FT   TRANSMEM        45..65
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   CONFLICT        81
FT                   /note="T -> V (in Ref. 2; AA sequence and 3; AA sequence)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   318 AA;  33928 MW;  D7B4513F774CC2E9 CRC64;
     MKAVIAVFIT LMLTAVVAFQ PAASFSAPKC AGKMSMAIED APKKAAAAAL AALTTLSVIS
     PSFAITKEEL RSLDYLQVKG TGLANRCPEV KGTDSIKVKG AGQEIVDLCI EPKTFQVLEE
     TRNKKGELSK EFINTKLMTR QTYTLDGISG DLNVVDGKIQ FTELDGIDYA ATTVQKPGGE
     RVPFLFTVKE LVAKASAGGN TIAPGFQMGG KFVVPSYRTG LFLDPKGRGA TTGYDMAVAL
     PGIQSGVEGD EELFRENNKV FDVLSGQIEF EVNRVNLEEG EIGGVFVSTQ GSDTDMGSKS
     PETVLLKGIF YGRIADKE