ID   PSBO_PINST              Reviewed;         116 AA.
AC   P84718;
DT   27-JUN-2006, integrated into UniProtKB/Swiss-Prot.
DT   29-OCT-2014, sequence version 2.
DT   25-MAY-2022, entry version 43.
DE   RecName: Full=Putative oxygen-evolving enhancer protein 1;
DE            Short=OEE1;
DE   AltName: Full=33 kDa subunit of oxygen evolving system of photosystem II;
DE   AltName: Full=33 kDa thylakoid membrane protein;
DE   AltName: Full=OEC 33 kDa subunit;
DE   AltName: Full=PS2;
DE   Flags: Fragments;
GN   Name=PSBO {ECO:0000250|UniProtKB:P26320};
OS   Pinus strobus (Eastern white pine).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Pinopsida; Pinidae; Conifers I; Pinales; Pinaceae; Pinus;
OC   Pinus subgen. Strobus.
OX   NCBI_TaxID=3348;
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE.
RC   TISSUE=Leaf {ECO:0000269|PubMed:16529377};
RX   PubMed=16529377; DOI=10.1094/mpmi-19-0150;
RA   Smith J.A., Blanchette R.A., Burnes T.A., Jacobs J.J., Higgins L.,
RA   Witthuhn B.A., David A.J., Gillman J.H.;
RT   "Proteomic comparison of needles from blister rust-resistant and
RT   susceptible Pinus strobus seedlings reveals upregulation of putative
RT   disease resistance proteins.";
RL   Mol. Plant Microbe Interact. 19:150-160(2006).
RN   [2]
RP   CONTAMINATING SEQUENCE.
RX   PubMed=23895828; DOI=10.1016/j.jprot.2013.07.009;
RA   Nawrot R., Barylski J., Schulze W.X.;
RT   "Incorrectly annotated keratin derived peptide sequences lead to misleading
RT   MS/MS data interpretation.";
RL   J. Proteomics 91:270-273(2013).
CC   -!- FUNCTION: Stabilizes the manganese cluster which is the primary site of
CC       water splitting. {ECO:0000250|UniProtKB:P23321}.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane.
CC       Note=Associated with the photosystem II complex.
CC   -!- MISCELLANEOUS: On the 2D-gel the determined pI of this protein is: 5.7,
CC       its MW is: 35.1 kDa. {ECO:0000269|PubMed:16529377}.
CC   -!- SIMILARITY: Belongs to the PsbO family. {ECO:0000305}.
CC   -!- CAUTION: The order of the peptides shown is unknown.
CC       {ECO:0000269|PubMed:16529377}.
CC   -!- CAUTION: This sequence was originally thought to contain an additional
CC       peptide (PubMed:16529377). However, it was later shown that the peptide
CC       is likely to be human type II keratin, a common contaminant in
CC       proteomic analyses, so it has been removed and the remaining sequence
CC       may also contain further contaminations (PubMed:23895828).
CC       {ECO:0000305|PubMed:23895828}.
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DR   AlphaFoldDB; P84718; -.
DR   PRIDE; P84718; -.
DR   GO; GO:0009535; C:chloroplast thylakoid membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0009523; C:photosystem II; IEA:UniProtKB-KW.
DR   GO; GO:0015979; P:photosynthesis; IEA:UniProtKB-KW.
DR   InterPro; IPR011250; OMP/PagP_b-brl.
DR   SUPFAM; SSF56925; SSF56925; 1.
PE   1: Evidence at protein level;
KW   Chloroplast; Direct protein sequencing; Manganese; Membrane;
KW   Photosynthesis; Photosystem II; Plastid; Thylakoid.
FT   CHAIN           <1..>116
FT                   /note="Putative oxygen-evolving enhancer protein 1"
FT                   /id="PRO_0000240623"
FT   NON_CONS        17..18
FT                   /evidence="ECO:0000303|PubMed:16529377"
FT   NON_CONS        34..35
FT                   /evidence="ECO:0000303|PubMed:16529377"
FT   NON_CONS        42..43
FT                   /evidence="ECO:0000303|PubMed:16529377"
FT   NON_CONS        60..61
FT                   /evidence="ECO:0000303|PubMed:16529377"
FT   NON_CONS        70..71
FT                   /evidence="ECO:0000303|PubMed:16529377"
FT   NON_CONS        81..82
FT                   /evidence="ECO:0000303|PubMed:16529377"
FT   NON_CONS        92..93
FT                   /evidence="ECO:0000303|PubMed:16529377"
FT   NON_CONS        103..104
FT                   /evidence="ECO:0000303|PubMed:16529377"
FT   NON_TER         1
FT                   /evidence="ECO:0000303|PubMed:16529377"
FT   NON_TER         116
FT                   /evidence="ECO:0000303|PubMed:16529377"
SQ   SEQUENCE   116 AA;  12277 MW;  765CD68D51B2C9FB CRC64;
     DGIDYAAVTV QLPGGERGGS TGYDNAVALP AGGRGSSMLD PKELGQMNIV FEGVSKSYHD
     AVALVLPSLK ASTYYEESLY KVINTWADII NRALTEAVAA EAAAAEDPEM ETMYTK