PSBO_SPIOL
ID PSBO_SPIOL Reviewed; 332 AA.
AC P12359;
DT 01-OCT-1989, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1989, sequence version 1.
DT 25-MAY-2022, entry version 86.
DE RecName: Full=Oxygen-evolving enhancer protein 1, chloroplastic;
DE Short=OEE1;
DE AltName: Full=33 kDa subunit of oxygen evolving system of photosystem II;
DE AltName: Full=33 kDa thylakoid membrane protein;
DE AltName: Full=OEC 33 kDa subunit;
DE Flags: Precursor;
GN Name=PSBO;
OS Spinacia oleracea (Spinach).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC Caryophyllales; Chenopodiaceae; Chenopodioideae; Anserineae; Spinacia.
OX NCBI_TaxID=3562;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Tyagi A., Hermans J., Steppuhn R.C.J., Jansson C., Vater F., Herrmann R.G.;
RT "Nucleotide sequence of cDNA clones encoding the complete '33 kDa'
RT precursor protein associated with the photosynthetic oxygen-evolving
RT complex from spinach.";
RL Mol. Gen. Genet. 207:288-293(1987).
RN [2]
RP PROTEIN SEQUENCE OF 85-332.
RA Oh-Oka H., Tanaka S., Wada K., Kuwabara T., Murata N.;
RT "Complete amino acid sequence of 33 kDa protein isolated from spinach
RT photosystem II particles.";
RL FEBS Lett. 197:63-66(1986).
RN [3]
RP PROTEIN SEQUENCE OF 85-121.
RA Yamamoto Y., Hermodson M.A., Krogmann D.W.;
RT "Improved purification and N-terminal sequence of the 33-kDa protein in
RT spinach PS II.";
RL FEBS Lett. 195:155-158(1986).
RN [4]
RP PROTEIN SEQUENCE OF 85-91, SUBCELLULAR LOCATION, AND CHARACTERIZATION.
RX PubMed=14736920; DOI=10.1073/pnas.0308164100;
RA Spetea C., Hundal T., Lundin B., Heddad M., Adamska I., Andersson B.;
RT "Multiple evidence for nucleotide metabolism in the chloroplast thylakoid
RT lumen.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:1409-1414(2004).
CC -!- FUNCTION: Stabilizes the manganese cluster which is the primary site of
CC water splitting (By similarity). Binds GTP after preillumination of
CC photosystem II core complex. This binding is inhibited by DCMU.
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane
CC {ECO:0000269|PubMed:14736920}. Note=Associated with the photosystem II
CC complex.
CC -!- SIMILARITY: Belongs to the PsbO family. {ECO:0000305}.
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DR EMBL; X05548; CAA29062.1; -; mRNA.
DR PIR; A23613; A23613.
DR PIR; S00415; S00415.
DR PDB; 3JCU; EM; 3.20 A; O/o=1-332.
DR PDBsum; 3JCU; -.
DR AlphaFoldDB; P12359; -.
DR SMR; P12359; -.
DR DIP; DIP-62020N; -.
DR IntAct; P12359; 1.
DR PRIDE; P12359; -.
DR GO; GO:0030095; C:chloroplast photosystem II; IDA:CAFA.
DR GO; GO:0009654; C:photosystem II oxygen evolving complex; IMP:CAFA.
DR GO; GO:0010242; F:oxygen evolving activity; IMP:CAFA.
DR GO; GO:0019684; P:photosynthesis, light reaction; IMP:CAFA.
DR GO; GO:0010207; P:photosystem II assembly; IMP:CAFA.
DR GO; GO:0042549; P:photosystem II stabilization; IEA:InterPro.
DR DisProt; DP00188; -.
DR InterPro; IPR011250; OMP/PagP_b-brl.
DR InterPro; IPR002628; PSII_MSP.
DR PANTHER; PTHR34058; PTHR34058; 1.
DR Pfam; PF01716; MSP; 1.
DR SUPFAM; SSF56925; SSF56925; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Chloroplast; Direct protein sequencing; Manganese; Membrane;
KW Photosynthesis; Photosystem II; Plastid; Thylakoid; Transit peptide.
FT TRANSIT 1..?
FT /note="Chloroplast"
FT TRANSIT ?..84
FT /note="Thylakoid"
FT CHAIN 85..332
FT /note="Oxygen-evolving enhancer protein 1, chloroplastic"
FT /id="PRO_0000029561"
FT CONFLICT 96
FT /note="Q -> N (in Ref. 3; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 111
FT /note="Q -> E (in Ref. 3; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 120
FT /note="D -> K (in Ref. 3; AA sequence)"
FT /evidence="ECO:0000305"
FT HELIX 92..97
FT /evidence="ECO:0007829|PDB:3JCU"
FT HELIX 100..103
FT /evidence="ECO:0007829|PDB:3JCU"
FT TURN 104..106
FT /evidence="ECO:0007829|PDB:3JCU"
FT HELIX 108..110
FT /evidence="ECO:0007829|PDB:3JCU"
FT STRAND 136..144
FT /evidence="ECO:0007829|PDB:3JCU"
FT STRAND 161..163
FT /evidence="ECO:0007829|PDB:3JCU"
FT STRAND 194..200
FT /evidence="ECO:0007829|PDB:3JCU"
FT STRAND 206..212
FT /evidence="ECO:0007829|PDB:3JCU"
FT STRAND 217..220
FT /evidence="ECO:0007829|PDB:3JCU"
FT HELIX 222..224
FT /evidence="ECO:0007829|PDB:3JCU"
FT STRAND 225..231
FT /evidence="ECO:0007829|PDB:3JCU"
FT STRAND 245..253
FT /evidence="ECO:0007829|PDB:3JCU"
FT HELIX 258..263
FT /evidence="ECO:0007829|PDB:3JCU"
FT TURN 266..268
FT /evidence="ECO:0007829|PDB:3JCU"
FT HELIX 269..272
FT /evidence="ECO:0007829|PDB:3JCU"
FT STRAND 279..290
FT /evidence="ECO:0007829|PDB:3JCU"
FT TURN 292..294
FT /evidence="ECO:0007829|PDB:3JCU"
FT STRAND 296..302
FT /evidence="ECO:0007829|PDB:3JCU"
FT STRAND 318..328
FT /evidence="ECO:0007829|PDB:3JCU"
SQ SEQUENCE 332 AA; 35171 MW; B15507F5835117FF CRC64;
MAASLQASTT FLQPTKVASR NTLQLRSTQN VCKAFGVESA SSGGRLSLSL QSDLKELANK
CVDATKLAGL ALATSALIAS GANAEGGKRL TYDEIQSKTY LEVKGTGTAN QCPTVEGGVD
SFAFKPGKYT AKKFCLEPTK FAVKAEGISK NSGPDFQNTK LMTRLTYTLD EIEGPFEVSS
DGTVKFEEKD GIDYAAVTVQ LPGGERVPFL FTIKQLVASG KPESFSGDFL VPSYRGSSFL
DPKGRGGSTG YDNAVALPAG GRGDEEELQK ENNKNVASSK GTITLSVTSS KPETGEVIGV
FQSLQPSDTD LGAKVPKDVK IEGVWYAQLE QQ
