PSBO_SYNY3
ID PSBO_SYNY3 Reviewed; 274 AA.
AC P10549;
DT 01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-1989, sequence version 1.
DT 03-AUG-2022, entry version 125.
DE RecName: Full=Photosystem II manganese-stabilizing polypeptide;
DE Short=MSP;
DE Flags: Precursor;
GN Name=psbO; OrderedLocusNames=sll0427;
OS Synechocystis sp. (strain PCC 6803 / Kazusa).
OC Bacteria; Cyanobacteria; Synechococcales; Merismopediaceae; Synechocystis;
OC unclassified Synechocystis.
OX NCBI_TaxID=1111708;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=3138527; DOI=10.1007/bf00330845;
RA Philbrick J.B., Zilinskas B.A.;
RT "Cloning, nucleotide sequence and mutational analysis of the gene encoding
RT the Photosystem II manganese-stabilizing polypeptide of Synechocystis
RT 6803.";
RL Mol. Gen. Genet. 212:418-425(1988).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=PCC 6803 / Kazusa;
RX PubMed=8905231; DOI=10.1093/dnares/3.3.109;
RA Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y.,
RA Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T.,
RA Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S.,
RA Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence analysis of the genome of the unicellular cyanobacterium
RT Synechocystis sp. strain PCC6803. II. Sequence determination of the entire
RT genome and assignment of potential protein-coding regions.";
RL DNA Res. 3:109-136(1996).
RN [3]
RP PROTEIN SEQUENCE OF 29-39, IDENTIFICATION BY MASS SPECTROMETRY, SUBUNIT,
RP AND SUBCELLULAR LOCATION.
RC STRAIN=PCC 6803 / Kazusa;
RX PubMed=12069591; DOI=10.1021/bi026012+;
RA Kashino Y., Lauber W.M., Carroll J.A., Wang Q., Whitmarsh J., Satoh K.,
RA Pakrasi H.B.;
RT "Proteomic analysis of a highly active photosystem II preparation from the
RT cyanobacterium Synechocystis sp. PCC 6803 reveals the presence of novel
RT polypeptides.";
RL Biochemistry 41:8004-8012(2002).
RN [4]
RP PROTEIN SEQUENCE OF 29-37.
RX PubMed=9298645; DOI=10.1002/elps.1150180806;
RA Sazuka T., Ohara O.;
RT "Towards a proteome project of cyanobacterium Synechocystis sp. strain
RT PCC6803: linking 130 protein spots with their respective genes.";
RL Electrophoresis 18:1252-1258(1997).
CC -!- FUNCTION: MSP binds to a putative Mn-binding protein and keeps 2 of the
CC 4 Mn-atoms associated with PSII.
CC -!- SUBUNIT: Cyanobacterial PSII is composed of 1 copy each of membrane
CC proteins PsbA, PsbB, PsbC, PsbD, PsbE, PsbF, PsbH, PsbI, PsbJ, PsbK,
CC PsbL, PsbM, PsbT, PsbX, PsbY, PsbZ, Ycf12, at least 3 peripheral
CC proteins PsbO, PsbU, PsbV and a large number of cofactors. It forms
CC dimeric complexes. {ECO:0000269|PubMed:12069591}.
CC -!- SUBCELLULAR LOCATION: Cellular thylakoid membrane
CC {ECO:0000269|PubMed:12069591}; Peripheral membrane protein
CC {ECO:0000269|PubMed:12069591}; Lumenal side.
CC -!- SIMILARITY: Belongs to the PsbO family. {ECO:0000305}.
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DR EMBL; X07986; CAA30796.1; -; Genomic_DNA.
DR EMBL; BA000022; BAA18474.1; -; Genomic_DNA.
DR PIR; S01261; S01261.
DR PDB; 7N8O; EM; 1.93 A; O/o=28-274.
DR PDB; 7RCV; EM; 2.01 A; O/o=28-274.
DR PDBsum; 7N8O; -.
DR PDBsum; 7RCV; -.
DR AlphaFoldDB; P10549; -.
DR SMR; P10549; -.
DR IntAct; P10549; 6.
DR STRING; 1148.1653561; -.
DR PaxDb; P10549; -.
DR EnsemblBacteria; BAA18474; BAA18474; BAA18474.
DR KEGG; syn:sll0427; -.
DR eggNOG; ENOG502Z7ZP; Bacteria.
DR InParanoid; P10549; -.
DR OMA; GKANDCP; -.
DR PhylomeDB; P10549; -.
DR BioCyc; MetaCyc:PSBO-MON; -.
DR Proteomes; UP000001425; Chromosome.
DR GO; GO:0009654; C:photosystem II oxygen evolving complex; IEA:InterPro.
DR GO; GO:0031676; C:plasma membrane-derived thylakoid membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0030096; C:plasma membrane-derived thylakoid photosystem II; IDA:UniProtKB.
DR GO; GO:0010242; F:oxygen evolving activity; IEA:InterPro.
DR GO; GO:0010207; P:photosystem II assembly; IEA:InterPro.
DR GO; GO:0042549; P:photosystem II stabilization; IEA:InterPro.
DR InterPro; IPR011250; OMP/PagP_b-brl.
DR InterPro; IPR002628; PSII_MSP.
DR PANTHER; PTHR34058; PTHR34058; 1.
DR Pfam; PF01716; MSP; 1.
DR SUPFAM; SSF56925; SSF56925; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Direct protein sequencing; Manganese; Membrane;
KW Photosynthesis; Photosystem II; Reference proteome; Signal; Thylakoid.
FT SIGNAL 1..28
FT /evidence="ECO:0000269|PubMed:12069591,
FT ECO:0000269|PubMed:9298645"
FT CHAIN 29..274
FT /note="Photosystem II manganese-stabilizing polypeptide"
FT /id="PRO_0000029570"
FT HELIX 36..39
FT /evidence="ECO:0007829|PDB:7N8O"
FT TURN 40..42
FT /evidence="ECO:0007829|PDB:7N8O"
FT HELIX 44..46
FT /evidence="ECO:0007829|PDB:7N8O"
FT STRAND 66..82
FT /evidence="ECO:0007829|PDB:7N8O"
FT STRAND 86..90
FT /evidence="ECO:0007829|PDB:7N8O"
FT STRAND 94..96
FT /evidence="ECO:0007829|PDB:7N8O"
FT STRAND 98..101
FT /evidence="ECO:0007829|PDB:7RCV"
FT STRAND 107..116
FT /evidence="ECO:0007829|PDB:7N8O"
FT STRAND 118..120
FT /evidence="ECO:0007829|PDB:7N8O"
FT STRAND 122..130
FT /evidence="ECO:0007829|PDB:7N8O"
FT STRAND 132..138
FT /evidence="ECO:0007829|PDB:7N8O"
FT STRAND 144..150
FT /evidence="ECO:0007829|PDB:7N8O"
FT STRAND 155..157
FT /evidence="ECO:0007829|PDB:7N8O"
FT STRAND 163..165
FT /evidence="ECO:0007829|PDB:7N8O"
FT STRAND 170..177
FT /evidence="ECO:0007829|PDB:7N8O"
FT STRAND 193..199
FT /evidence="ECO:0007829|PDB:7N8O"
FT HELIX 204..209
FT /evidence="ECO:0007829|PDB:7N8O"
FT HELIX 211..213
FT /evidence="ECO:0007829|PDB:7N8O"
FT STRAND 220..232
FT /evidence="ECO:0007829|PDB:7N8O"
FT TURN 233..236
FT /evidence="ECO:0007829|PDB:7N8O"
FT STRAND 237..247
FT /evidence="ECO:0007829|PDB:7N8O"
FT TURN 251..254
FT /evidence="ECO:0007829|PDB:7N8O"
FT STRAND 259..270
FT /evidence="ECO:0007829|PDB:7N8O"
SQ SEQUENCE 274 AA; 29912 MW; DFFC7B5E7D993C11 CRC64;
MRFRPSIVAL LSVCFGLLTF LYSGSAFAVD KSQLTYDDIV NTGLANVCPE ISSFTRGTIE
VEPNTKYFVS DFCMEPQEYF VKEEPVNKRQ KAEYVKGKVL TRQTTSLEQI RGSIAVGADG
TLTFKEKDGI DFQPITVLLP GGEEVPFFFT VKNFTGTTEP GFTSINSSTD FVGDFNVPSY
RGAGFLDPKA RGLYTGYDNA VALPSAADKF RTNKKETPLG KGTLSLQVTQ VDGSTGEIAG
IFESEQPSDT DLGAKEPLDV KVRGIFYGRV DTDV
