PSBQ2_ARATH
ID PSBQ2_ARATH Reviewed; 230 AA.
AC Q41932; Q9M0X7;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2000, sequence version 2.
DT 03-AUG-2022, entry version 151.
DE RecName: Full=Oxygen-evolving enhancer protein 3-2, chloroplastic;
DE Short=OEE3;
DE AltName: Full=16 kDa subunit of oxygen evolving system of photosystem II;
DE AltName: Full=OEC 16 kDa subunit;
DE Flags: Precursor;
GN Name=PSBQ2; Synonyms=PSBQB; OrderedLocusNames=At4g05180;
GN ORFNames=C17L7.100;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 166-230.
RC STRAIN=cv. Columbia; TISSUE=Green siliques;
RX PubMed=8281187; DOI=10.1046/j.1365-313x.1993.04061051.x;
RA Hoefte H., Desprez T., Amselem J., Chiapello H., Rouze P., Caboche M.,
RA Moisan A., Jourjon M.-F., Charpenteau J.-L., Berthomieu P., Guerrier D.,
RA Giraudat J., Quigley F., Thomas F., Yu D.-Y., Mache R., Raynal M.,
RA Cooke R., Grellet F., Delseny M., Parmentier Y., de Marcillac G., Gigot C.,
RA Fleck J., Philipps G., Axelos M., Bardet C., Tremousaygue D., Lescure B.;
RT "An inventory of 1152 expressed sequence tags obtained by partial
RT sequencing of cDNAs from Arabidopsis thaliana.";
RL Plant J. 4:1051-1061(1993).
RN [6]
RP PROTEIN SEQUENCE OF 50-57, AND TRANSIT PEPTIDE.
RX PubMed=23146587; DOI=10.1016/j.ab.2012.10.040;
RA Candat A., Poupart P., Andrieu J.P., Chevrollier A., Reynier P.,
RA Rogniaux H., Avelange-Macherel M.H., Macherel D.;
RT "Experimental determination of organelle targeting-peptide cleavage sites
RT using transient expression of green fluorescent protein translational
RT fusions.";
RL Anal. Biochem. 434:44-51(2013).
RN [7]
RP PROTEIN SEQUENCE OF 83-96, AND SUBCELLULAR LOCATION.
RX PubMed=11719511; DOI=10.1074/jbc.m108575200;
RA Schubert M., Petersson U.A., Haas B.J., Funk C., Schroeder W.P.,
RA Kieselbach T.;
RT "Proteome map of the chloroplast lumen of Arabidopsis thaliana.";
RL J. Biol. Chem. 277:8354-8365(2002).
RN [8]
RP PROTEIN SEQUENCE OF N-TERMINUS, AND IDENTIFICATION BY MASS SPECTROMETRY.
RX PubMed=11826309; DOI=10.1105/tpc.010304;
RA Peltier J.-B., Emanuelsson O., Kalume D.E., Ytterberg J., Friso G.,
RA Rudella A., Liberles D.A., Soederberg L., Roepstorff P., von Heijne G.,
RA van Wijk K.J.;
RT "Central functions of the lumenal and peripheral thylakoid proteome of
RT Arabidopsis determined by experimentation and genome-wide prediction.";
RL Plant Cell 14:211-236(2002).
RN [9]
RP FUNCTION.
RX PubMed=16822865; DOI=10.1074/jbc.m603582200;
RA Yi X., Hargett S.R., Frankel L.K., Bricker T.M.;
RT "The PsbQ protein is required in Arabidopsis for photosystem II
RT assembly/stability and photoautotrophy under low light conditions.";
RL J. Biol. Chem. 281:26260-26267(2006).
RN [10]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND SUBCELLULAR LOCATION [LARGE SCALE
RP ANALYSIS].
RX PubMed=18431481; DOI=10.1371/journal.pone.0001994;
RA Zybailov B., Rutschow H., Friso G., Rudella A., Emanuelsson O., Sun Q.,
RA van Wijk K.J.;
RT "Sorting signals, N-terminal modifications and abundance of the chloroplast
RT proteome.";
RL PLoS ONE 3:E1994-E1994(2008).
RN [11]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-125, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19376835; DOI=10.1104/pp.109.138677;
RA Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A.,
RA Grossmann J., Gruissem W., Baginsky S.;
RT "Large-scale Arabidopsis phosphoproteome profiling reveals novel
RT chloroplast kinase substrates and phosphorylation networks.";
RL Plant Physiol. 150:889-903(2009).
RN [12]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-216, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22092075; DOI=10.1021/pr200917t;
RA Aryal U.K., Krochko J.E., Ross A.R.;
RT "Identification of phosphoproteins in Arabidopsis thaliana leaves using
RT polyethylene glycol fractionation, immobilized metal-ion affinity
RT chromatography, two-dimensional gel electrophoresis and mass
RT spectrometry.";
RL J. Proteome Res. 11:425-437(2012).
CC -!- FUNCTION: Required for photosystem II assembly/stability and
CC photoautotrophic growth under low light conditions.
CC {ECO:0000269|PubMed:16822865}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane
CC {ECO:0000269|PubMed:11719511, ECO:0000269|PubMed:18431481}; Peripheral
CC membrane protein {ECO:0000269|PubMed:11719511}; Lumenal side
CC {ECO:0000269|PubMed:11719511}. Note=Associated with the photosystem II
CC complex.
CC -!- SIMILARITY: Belongs to the psbQ family. {ECO:0000305}.
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DR EMBL; AL161503; CAB81060.1; -; Genomic_DNA.
DR EMBL; CP002687; AEE82488.1; -; Genomic_DNA.
DR EMBL; CP002687; ANM66257.1; -; Genomic_DNA.
DR EMBL; AF372897; AAK49613.1; -; mRNA.
DR EMBL; BT000648; AAN18214.1; -; mRNA.
DR EMBL; AY088008; AAM65554.1; -; mRNA.
DR EMBL; Z17777; CAA79064.1; -; mRNA.
DR PIR; B85065; B85065.
DR RefSeq; NP_001319873.1; NM_001340541.1.
DR RefSeq; NP_192427.1; NM_116757.4.
DR AlphaFoldDB; Q41932; -.
DR SMR; Q41932; -.
DR BioGRID; 11177; 7.
DR IntAct; Q41932; 2.
DR MINT; Q41932; -.
DR STRING; 3702.AT4G05180.1; -.
DR iPTMnet; Q41932; -.
DR PaxDb; Q41932; -.
DR PRIDE; Q41932; -.
DR ProteomicsDB; 226099; -.
DR EnsemblPlants; AT4G05180.1; AT4G05180.1; AT4G05180.
DR EnsemblPlants; AT4G05180.2; AT4G05180.2; AT4G05180.
DR GeneID; 825866; -.
DR Gramene; AT4G05180.1; AT4G05180.1; AT4G05180.
DR Gramene; AT4G05180.2; AT4G05180.2; AT4G05180.
DR KEGG; ath:AT4G05180; -.
DR Araport; AT4G05180; -.
DR TAIR; locus:2115703; AT4G05180.
DR eggNOG; ENOG502QQF9; Eukaryota.
DR HOGENOM; CLU_085524_0_0_1; -.
DR InParanoid; Q41932; -.
DR OMA; DRFYIQP; -.
DR OrthoDB; 1248380at2759; -.
DR PhylomeDB; Q41932; -.
DR BioCyc; ARA:AT4G05180-MON; -.
DR PRO; PR:Q41932; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; Q41932; baseline and differential.
DR Genevisible; Q41932; AT.
DR GO; GO:0048046; C:apoplast; HDA:TAIR.
DR GO; GO:0009507; C:chloroplast; HDA:TAIR.
DR GO; GO:0009570; C:chloroplast stroma; HDA:TAIR.
DR GO; GO:0009534; C:chloroplast thylakoid; HDA:TAIR.
DR GO; GO:0009535; C:chloroplast thylakoid membrane; HDA:TAIR.
DR GO; GO:0005829; C:cytosol; HDA:TAIR.
DR GO; GO:0019898; C:extrinsic component of membrane; IEA:InterPro.
DR GO; GO:0009654; C:photosystem II oxygen evolving complex; IEA:InterPro.
DR GO; GO:0009579; C:thylakoid; HDA:TAIR.
DR GO; GO:0031977; C:thylakoid lumen; HDA:TAIR.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR GO; GO:0045156; F:electron transporter, transferring electrons within the cyclic electron transport pathway of photosynthesis activity; IBA:GO_Central.
DR GO; GO:0009767; P:photosynthetic electron transport chain; IBA:GO_Central.
DR Gene3D; 1.20.120.290; -; 1.
DR InterPro; IPR023222; PsbQ-like_dom_sf.
DR InterPro; IPR008797; PSII_PsbQ.
DR PANTHER; PTHR33399; PTHR33399; 1.
DR Pfam; PF05757; PsbQ; 1.
DR SUPFAM; SSF101112; SSF101112; 1.
PE 1: Evidence at protein level;
KW Chloroplast; Direct protein sequencing; Membrane; Phosphoprotein;
KW Photosynthesis; Photosystem II; Plastid; Reference proteome; Thylakoid;
KW Transit peptide.
FT TRANSIT 1..49
FT /note="Chloroplast"
FT TRANSIT 50..82
FT /note="Thylakoid"
FT CHAIN 83..230
FT /note="Oxygen-evolving enhancer protein 3-2, chloroplastic"
FT /id="PRO_0000029590"
FT MOD_RES 125
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19376835"
FT MOD_RES 195
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:Q9XFT3"
FT MOD_RES 215
FT /note="Phosphotyrosine"
FT /evidence="ECO:0000250|UniProtKB:Q9XFT3"
FT MOD_RES 216
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:22092075"
FT MOD_RES 218
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:Q9XFT3"
FT CONFLICT 190
FT /note="A -> T (in Ref. 5; CAA79064)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 230 AA; 24643 MW; EE4D6BA7442038A0 CRC64;
MAQAVTSMAG LRGASQAVLE GSLQINGSNR LNISRVSVGS QRTGLVIRAQ QNVSVPESSR
RSVIGLVAAG LAGGSFVKAV FAEAIPIKVG GPPLPSGGLP GTDNSDQARD FSLALKDRFY
IQPLSPTEAA ARAKDSAKEI INVKSFIDKK AWPYVQNDLR LRASYLRYDL NTVISAKPKE
EKQSLKDLTA KLFQTIDNLD YAARSKSSPD AEKYYSETVS SLNNVLAKLG
