PSBU_SYNY3
ID PSBU_SYNY3 Reviewed; 131 AA.
AC Q55332; P72832;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 2.
DT 03-AUG-2022, entry version 126.
DE RecName: Full=Photosystem II 12 kDa extrinsic protein;
DE AltName: Full=PS II complex 12 kDa extrinsic protein;
DE AltName: Full=PSII-U;
DE Flags: Precursor;
GN Name=psbU; OrderedLocusNames=sll1194;
OS Synechocystis sp. (strain PCC 6803 / Kazusa).
OC Bacteria; Cyanobacteria; Synechococcales; Merismopediaceae; Synechocystis;
OC unclassified Synechocystis.
OX NCBI_TaxID=1111708;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 37-80.
RX PubMed=9211937; DOI=10.1074/jbc.272.28.17821;
RA Shen J.-R., Ikeuchi M., Inoue Y.;
RT "Analysis of the psbU gene encoding the 12-kDa extrinsic protein of
RT photosystem II and studies on its role by deletion mutagenesis in
RT Synechocystis sp. PCC 6803.";
RL J. Biol. Chem. 272:17821-17826(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=PCC 6803 / Kazusa;
RX PubMed=8905231; DOI=10.1093/dnares/3.3.109;
RA Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y.,
RA Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T.,
RA Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S.,
RA Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence analysis of the genome of the unicellular cyanobacterium
RT Synechocystis sp. strain PCC6803. II. Sequence determination of the entire
RT genome and assignment of potential protein-coding regions.";
RL DNA Res. 3:109-136(1996).
RN [3]
RP PROTEIN SEQUENCE OF 37-52, IDENTIFICATION BY MASS SPECTROMETRY, SUBUNIT,
RP AND SUBCELLULAR LOCATION.
RC STRAIN=PCC 6803 / Kazusa;
RX PubMed=12069591; DOI=10.1021/bi026012+;
RA Kashino Y., Lauber W.M., Carroll J.A., Wang Q., Whitmarsh J., Satoh K.,
RA Pakrasi H.B.;
RT "Proteomic analysis of a highly active photosystem II preparation from the
RT cyanobacterium Synechocystis sp. PCC 6803 reveals the presence of novel
RT polypeptides.";
RL Biochemistry 41:8004-8012(2002).
RN [4]
RP CHARACTERIZATION.
RX PubMed=16142920; DOI=10.1021/bi047539k;
RA Inoue-Kashino N., Kashino Y., Satoh K., Terashima I., Pakrasi H.B.;
RT "PsbU provides a stable architecture for the oxygen-evolving system in
RT cyanobacterial photosystem II.";
RL Biochemistry 44:12214-12228(2005).
CC -!- FUNCTION: Stabilizes the structure of photosystem II oxygen-evolving
CC complex (OEC), the ion environment of oxygen evolution and protects the
CC OEC against heat-induced inactivation. May modulate the Cl(-)
CC requirement for oxygen evolution.
CC -!- SUBUNIT: With PsbO,P,Q and V forms the oxygen-evolving complex of
CC photosystem II. {ECO:0000250, ECO:0000269|PubMed:12069591}.
CC -!- SUBCELLULAR LOCATION: Cellular thylakoid membrane
CC {ECO:0000269|PubMed:12069591}; Peripheral membrane protein
CC {ECO:0000269|PubMed:12069591}; Lumenal side. Note=Associated with
CC photosystem II at the lumenal side of the thylakoid membrane.
CC {ECO:0000269|PubMed:12069591}.
CC -!- SIMILARITY: Belongs to the PsbU family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; D84098; BAA12221.1; -; Genomic_DNA.
DR EMBL; BA000022; BAA16847.1; -; Genomic_DNA.
DR PIR; S74696; S74696.
DR PDB; 7N8O; EM; 1.93 A; U/u=1-131.
DR PDB; 7RCV; EM; 2.01 A; U/u=1-131.
DR PDBsum; 7N8O; -.
DR PDBsum; 7RCV; -.
DR AlphaFoldDB; Q55332; -.
DR SMR; Q55332; -.
DR IntAct; Q55332; 1.
DR STRING; 1148.1651921; -.
DR PaxDb; Q55332; -.
DR EnsemblBacteria; BAA16847; BAA16847; BAA16847.
DR KEGG; syn:sll1194; -.
DR eggNOG; COG1555; Bacteria.
DR InParanoid; Q55332; -.
DR OMA; LLGCWGW; -.
DR PhylomeDB; Q55332; -.
DR BioCyc; MetaCyc:PSBU-MON; -.
DR Proteomes; UP000001425; Chromosome.
DR GO; GO:0019898; C:extrinsic component of membrane; IEA:InterPro.
DR GO; GO:0009654; C:photosystem II oxygen evolving complex; IEA:InterPro.
DR GO; GO:0031676; C:plasma membrane-derived thylakoid membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0030096; C:plasma membrane-derived thylakoid photosystem II; IDA:UniProtKB.
DR GO; GO:0015979; P:photosynthesis; IEA:UniProtKB-UniRule.
DR GO; GO:0042549; P:photosystem II stabilization; IEA:InterPro.
DR HAMAP; MF_00589; PSII_PsbU; 1.
DR InterPro; IPR010527; PSII_PsbU.
DR Pfam; PF06514; PsbU; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Direct protein sequencing; Electron transport; Membrane;
KW Photosynthesis; Photosystem II; Reference proteome; Signal; Thylakoid;
KW Transport.
FT SIGNAL 1..28
FT /evidence="ECO:0000255"
FT PROPEP 29..36
FT /evidence="ECO:0000269|PubMed:12069591,
FT ECO:0000269|PubMed:9211937"
FT /id="PRO_0000029607"
FT CHAIN 37..131
FT /note="Photosystem II 12 kDa extrinsic protein"
FT /id="PRO_0000029608"
FT CONFLICT 13
FT /note="L -> V (in Ref. 1; BAA12221)"
FT /evidence="ECO:0000305"
FT HELIX 41..46
FT /evidence="ECO:0007829|PDB:7N8O"
FT HELIX 48..50
FT /evidence="ECO:0007829|PDB:7N8O"
FT STRAND 52..54
FT /evidence="ECO:0007829|PDB:7N8O"
FT TURN 55..57
FT /evidence="ECO:0007829|PDB:7N8O"
FT HELIX 60..63
FT /evidence="ECO:0007829|PDB:7N8O"
FT TURN 67..71
FT /evidence="ECO:0007829|PDB:7N8O"
FT HELIX 72..79
FT /evidence="ECO:0007829|PDB:7N8O"
FT STRAND 84..86
FT /evidence="ECO:0007829|PDB:7N8O"
FT HELIX 87..91
FT /evidence="ECO:0007829|PDB:7N8O"
FT HELIX 97..106
FT /evidence="ECO:0007829|PDB:7N8O"
FT HELIX 107..109
FT /evidence="ECO:0007829|PDB:7N8O"
FT HELIX 117..120
FT /evidence="ECO:0007829|PDB:7N8O"
FT HELIX 121..123
FT /evidence="ECO:0007829|PDB:7N8O"
FT TURN 124..126
FT /evidence="ECO:0007829|PDB:7N8O"
SQ SEQUENCE 131 AA; 14245 MW; 9D1BB1B8EBA4A03B CRC64;
MKFISRLLVA CSLLIGLMGF LGADLAQALT PNPILAELNA VDAKLTTDFG QKIDLNNSDI
RDFRGLRGFY PNLASEIIKN APYDTVEEVL DIPGLSETQK SRLEANLGSF TVTEPSIELT
SGDDRINPGV Y
