PSBW_CHLRE
ID PSBW_CHLRE Reviewed; 115 AA.
AC Q9SPI9; Q7M1J4;
DT 23-JAN-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 97.
DE RecName: Full=Photosystem II reaction center W protein, chloroplastic;
DE AltName: Full=PSII 6.1 kDa protein;
DE Flags: Precursor;
GN Name=psbW;
OS Chlamydomonas reinhardtii (Chlamydomonas smithii).
OC Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Chlorophyceae;
OC CS clade; Chlamydomonadales; Chlamydomonadaceae; Chlamydomonas.
OX NCBI_TaxID=3055;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=cw15;
RA Bishop C.L., Cain A.J., Purton S., Nugent J.H.A.;
RT "Molecular cloning and sequence analysis of the Chlamydomonas reinhardtii
RT nuclear gene encoding the photosystem II subunit PsbW.";
RL (er) Plant Gene Register PGR99-141(1999).
RN [2]
RP PROTEIN SEQUENCE OF 60-75, IDENTIFICATION AS A SUBUNIT OF PHOTOSYSTEM II,
RP SUBUNIT, AND SUBCELLULAR LOCATION.
RC STRAIN=F54-14;
RX PubMed=1885590; DOI=10.1016/s0021-9258(18)55345-x;
RA de Vitry C., Diner B.A., Popo J.-L.;
RT "Photosystem II particles from Chlamydomonas reinhardtii. Purification,
RT molecular weight, small subunit composition, and protein phosphorylation.";
RL J. Biol. Chem. 266:16614-16621(1991).
RN [3]
RP CONFIRMATION AS A SUBUNIT OF PHOTOSYSTEM II, SUBUNIT, SUBCELLULAR LOCATION,
RP AND DEVELOPMENTAL STAGE.
RC STRAIN=2137;
RX PubMed=12856936; DOI=10.1023/a:1023963113290;
RA Bishop C.L., Purton S., Nugent J.H.A.;
RT "Molecular analysis of the Chlamydomonas nuclear gene encoding PsbW and
RT demonstration that PsbW is a subunit of photosystem II, but not photosystem
RT I.";
RL Plant Mol. Biol. 52:285-289(2003).
CC -!- FUNCTION: Stabilizes dimeric photosystem II (PSII). In its absence
CC there is a reduction of monomeric PSII (By similarity).
CC {ECO:0000250|UniProtKB:Q39194}.
CC -!- SUBUNIT: Part of the photosystem II complex. PSII is composed of 1 copy
CC each of membrane proteins PsbA, PsbB, PsbC, PsbD, numerous small
CC proteins, at least 3 peripheral proteins of the oxygen-evolving complex
CC and a large number of cofactors. It forms dimeric complexes.
CC {ECO:0000269|PubMed:12856936, ECO:0000269|PubMed:1885590}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane
CC {ECO:0000269|PubMed:12856936, ECO:0000269|PubMed:1885590}; Single-pass
CC membrane protein. Note=The N-terminus is found within the thylakoid
CC lumen (By similarity). {ECO:0000250|UniProtKB:Q41387}.
CC -!- DEVELOPMENTAL STAGE: Present in dark-grown plants it increases upon
CC exposure to light. {ECO:0000269|PubMed:12856936}.
CC -!- SIMILARITY: Belongs to the psbW family. {ECO:0000305}.
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DR EMBL; AF170026; AAD50464.1; -; Genomic_DNA.
DR PIR; A41170; A41170.
DR RefSeq; XP_001702016.1; XM_001701964.1.
DR PDB; 6KAC; EM; 2.70 A; W/w=1-115.
DR PDB; 6KAD; EM; 3.40 A; W/w=1-115.
DR PDB; 6KAF; EM; 3.73 A; W/w=1-115.
DR PDBsum; 6KAC; -.
DR PDBsum; 6KAD; -.
DR PDBsum; 6KAF; -.
DR AlphaFoldDB; Q9SPI9; -.
DR SMR; Q9SPI9; -.
DR STRING; 3055.EDO97291; -.
DR eggNOG; ENOG502SDRE; Eukaryota.
DR HOGENOM; CLU_2076233_0_0_1; -.
DR OMA; KCAVAAK; -.
DR OrthoDB; 1650754at2759; -.
DR BioCyc; MetaCyc:CHLREDRAFT_155150-MON; -.
DR GO; GO:0009535; C:chloroplast thylakoid membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0009523; C:photosystem II; IEA:UniProtKB-KW.
DR GO; GO:0015979; P:photosynthesis; IEA:UniProtKB-KW.
DR InterPro; IPR009806; PSII_PsbW_class2.
DR PANTHER; PTHR34552; PTHR34552; 1.
DR Pfam; PF07123; PsbW; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Chloroplast; Direct protein sequencing; Membrane;
KW Photosynthesis; Photosystem II; Plastid; Thylakoid; Transit peptide;
KW Transmembrane; Transmembrane helix.
FT TRANSIT 1..30
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT TRANSIT 31..59
FT /note="Thylakoid"
FT /evidence="ECO:0000269|PubMed:1885590"
FT CHAIN 60..115
FT /note="Photosystem II reaction center W protein,
FT chloroplastic"
FT /id="PRO_0000005339"
FT TOPO_DOM 60..78
FT /note="Lumenal, thylakoid"
FT /evidence="ECO:0000250"
FT TRANSMEM 79..99
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 100..115
FT /note="Stromal"
FT /evidence="ECO:0000250"
FT CONFLICT 72
FT /note="R -> L (in Ref. 2; AA sequence)"
FT /evidence="ECO:0000305"
FT STRAND 68..70
FT /evidence="ECO:0007829|PDB:6KAC"
FT HELIX 73..75
FT /evidence="ECO:0007829|PDB:6KAC"
FT HELIX 79..98
FT /evidence="ECO:0007829|PDB:6KAC"
FT HELIX 99..102
FT /evidence="ECO:0007829|PDB:6KAC"
FT STRAND 112..114
FT /evidence="ECO:0007829|PDB:6KAC"
SQ SEQUENCE 115 AA; 12141 MW; F83607FEE23E35DA CRC64;
MQALSARAPR VAAKPVSRSG ARSAVTVVCK ATTVRSEVAK KVAMLSTLPA TLAAHPAFAL
VDERMNGDGT GRPFGVNDPV LGWVLLGVFG TMWAIWFIGQ KDLGDFEDAD DGLKL
