ID   ATL61_ORYSJ             Reviewed;         251 AA.
AC   Q5Z5F2; A0A0P0WXD8;
DT   05-OCT-2010, integrated into UniProtKB/Swiss-Prot.
DT   23-NOV-2004, sequence version 1.
DT   03-AUG-2022, entry version 116.
DE   RecName: Full=E3 ubiquitin-protein ligase Os06g0535400;
DE            EC=2.3.2.27 {ECO:0000305};
DE   AltName: Full=RING-H2 finger protein Os06g0535400;
DE   AltName: Full=RING-type E3 ubiquitin transferase Os06g0535400 {ECO:0000305};
GN   OrderedLocusNames=Os06g0535400, LOC_Os06g34450; ORFNames=OSJNBa0001B21.23;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12869764; DOI=10.1126/science.1081288;
RG   The rice full-length cDNA consortium;
RT   "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT   japonica rice.";
RL   Science 301:376-379(2003).
RN   [5]
RP   FUNCTION.
RX   PubMed=16186120; DOI=10.1074/jbc.m411127200;
RA   Katoh S., Tsunoda Y., Murata K., Minami E., Katoh E.;
RT   "Active site residues and amino acid specificity of the ubiquitin carrier
RT   protein-binding RING-H2 finger domain.";
RL   J. Biol. Chem. 280:41015-41024(2005).
CC   -!- FUNCTION: Possesses E3 ubiquitin-protein ligase in vitro.
CC       {ECO:0000269|PubMed:16186120}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC         [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC         cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC         EC=2.3.2.27; Evidence={ECO:0000305};
CC   -!- PATHWAY: Protein modification; protein ubiquitination.
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
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DR   EMBL; AP005723; BAD54617.1; -; Genomic_DNA.
DR   EMBL; AP008212; BAF19705.1; -; Genomic_DNA.
DR   EMBL; AP014962; BAS98092.1; -; Genomic_DNA.
DR   EMBL; AK066960; BAG90201.1; -; mRNA.
DR   RefSeq; XP_015641590.1; XM_015786104.1.
DR   AlphaFoldDB; Q5Z5F2; -.
DR   SMR; Q5Z5F2; -.
DR   PaxDb; Q5Z5F2; -.
DR   PRIDE; Q5Z5F2; -.
DR   EnsemblPlants; Os06t0535400-01; Os06t0535400-01; Os06g0535400.
DR   GeneID; 4341214; -.
DR   Gramene; Os06t0535400-01; Os06t0535400-01; Os06g0535400.
DR   KEGG; osa:4341214; -.
DR   eggNOG; KOG0800; Eukaryota.
DR   HOGENOM; CLU_099207_0_0_1; -.
DR   InParanoid; Q5Z5F2; -.
DR   OMA; MREDQAL; -.
DR   OrthoDB; 1439243at2759; -.
DR   UniPathway; UPA00143; -.
DR   Proteomes; UP000000763; Chromosome 6.
DR   Proteomes; UP000059680; Chromosome 6.
DR   Genevisible; Q5Z5F2; OS.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0004842; F:ubiquitin-protein transferase activity; IDA:UniProtKB.
DR   GO; GO:0044260; P:cellular macromolecule metabolic process; IEA:UniProt.
DR   GO; GO:0016567; P:protein ubiquitination; IDA:UniProtKB.
DR   Gene3D; 3.30.40.10; -; 1.
DR   InterPro; IPR001841; Znf_RING.
DR   InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR   Pfam; PF13639; zf-RING_2; 1.
DR   SMART; SM00184; RING; 1.
DR   PROSITE; PS50089; ZF_RING_2; 1.
PE   2: Evidence at transcript level;
KW   Membrane; Metal-binding; Reference proteome; Transferase; Transmembrane;
KW   Transmembrane helix; Ubl conjugation pathway; Zinc; Zinc-finger.
FT   CHAIN           1..251
FT                   /note="E3 ubiquitin-protein ligase Os06g0535400"
FT                   /id="PRO_0000397688"
FT   TRANSMEM        28..48
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        102..122
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        127..147
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   ZN_FING         185..227
FT                   /note="RING-type; atypical"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00175"
SQ   SEQUENCE   251 AA;  26287 MW;  5AC09D9B7941288E CRC64;
     MELPWLDLPF TLLTLLLATR LAYDYYGVVA ATFTGSFSLQ IFLFYCFARW YRHTIAARAA
     ADADGDGGGG AVADEEAAPP VLIPLLEGRG GGGGGAGAAS SLANRCFAVV FMVFVPLVIV
     VFERSQADVV AYALCLANIL VMVVWLSPDA AADPASAAKS FLRLSDDEDE GSCSGSGHGA
     AEDKCCVCLA GMREAQALRD LPRCGHRFHA KCIGKWLTAH PTCPVCRTTA VPPPAPLPAS
     GDHADDAITP V