ID   PSB_PSEBL               Reviewed;          23 AA.
AC   P86915;
DT   28-MAR-2018, integrated into UniProtKB/Swiss-Prot.
DT   28-MAR-2018, sequence version 1.
DT   25-MAY-2022, entry version 7.
DE   RecName: Full=Pseudin-B {ECO:0000303|PubMed:23029273};
OS   Pseudis bolbodactyla (Frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Neobatrachia; Hyloidea; Hylidae; Hylinae; Dendropsophini;
OC   Pseudis.
OX   NCBI_TaxID=428381 {ECO:0000303|PubMed:23029273};
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE, SUBCELLULAR LOCATION, AND AMIDATION AT ALA-23.
RC   TISSUE=Skin secretion {ECO:0000303|Ref.1};
RA   Silva L.R., Bloch C. Jr.;
RL   Submitted (APR-2011) to UniProtKB.
RN   [2] {ECO:0000305}
RP   FUNCTION.
RX   PubMed=23029273; DOI=10.1371/journal.pone.0045848;
RA   Brand G.D., Magalhaes M.T., Tinoco M.L., Aragao F.J., Nicoli J.,
RA   Kelly S.M., Cooper A., Bloch C. Jr.;
RT   "Probing protein sequences as sources for encrypted antimicrobial
RT   peptides.";
RL   PLoS ONE 7:E45848-E45848(2012).
CC   -!- FUNCTION: Antimicrobial peptide with activity against both Gram-
CC       positive and Gram-negative bacteria (PubMed:23029273). Has been tested
CC       against X.axonopodis pv. glycines (MIC<2 uM), E.coli ATCC 25922 (MIC=6
CC       uM), S.aureus ATCC 29313 (MIC=6 uM) and P.aeruginosa ATCC 27853 (MIC=50
CC       uM). {ECO:0000269|PubMed:23029273}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|Ref.1}.
CC   -!- TISSUE SPECIFICITY: Expressed by the skin glands. {ECO:0000305|Ref.1}.
CC   -!- SIMILARITY: Belongs to the frog skin active peptide (FSAP) family.
CC       Pseudin subfamily. {ECO:0000305}.
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DR   AlphaFoldDB; P86915; -.
DR   GO; GO:0005576; C:extracellular region; IDA:UniProtKB.
DR   GO; GO:0050829; P:defense response to Gram-negative bacterium; IDA:UniProtKB.
DR   GO; GO:0050830; P:defense response to Gram-positive bacterium; IDA:UniProtKB.
DR   GO; GO:0045087; P:innate immune response; IEA:UniProtKB-KW.
DR   GO; GO:0031640; P:killing of cells of another organism; IDA:UniProtKB.
DR   InterPro; IPR013156; Antimicrobial_19.
DR   Pfam; PF08225; Antimicrobial19; 1.
PE   1: Evidence at protein level;
KW   Amidation; Amphibian defense peptide; Antibiotic; Antimicrobial;
KW   Direct protein sequencing; Immunity; Innate immunity; Secreted.
FT   PEPTIDE         1..23
FT                   /note="Pseudin-B"
FT                   /evidence="ECO:0000269|Ref.1"
FT                   /id="PRO_0000443550"
FT   MOD_RES         23
FT                   /note="Alanine amide"
FT                   /evidence="ECO:0000269|Ref.1"
SQ   SEQUENCE   23 AA;  2539 MW;  DF3AAB64437B95C3 CRC64;
     GLNTLKKVIQ GLHEVIKLVN NHA