PSCA_MOUSE
ID PSCA_MOUSE Reviewed; 123 AA.
AC P57096; Q9D7U0;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2017, sequence version 2.
DT 03-AUG-2022, entry version 132.
DE RecName: Full=Prostate stem cell antigen;
DE Flags: Precursor;
GN Name=Psca;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE.
RC TISSUE=Fetus, and Kidney;
RX PubMed=9465086; DOI=10.1073/pnas.95.4.1735;
RA Reiter R.E., Gu Z., Watabe T., Thomas G., Szigeti K., Davis E., Wahl M.,
RA Nisitani S., Yamashiro J., le Beau M.M., Losa M., Witte O.N.;
RT "Prostate stem cell antigen: a cell surface marker overexpressed in
RT prostate cancer.";
RL Proc. Natl. Acad. Sci. U.S.A. 95:1735-1740(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=C57BL/6J;
RX PubMed=11479226;
RA Yang D., Holt G.E., Velders M.P., Kwon E.D., Kast W.M.;
RT "Murine six-transmembrane epithelial antigen of the prostate, prostate stem
RT cell antigen, and prostate-specific membrane antigen: prostate-specific
RT cell-surface antigens highly expressed in prostate cancer of transgenic
RT adenocarcinoma mouse prostate mice.";
RL Cancer Res. 61:5857-5860(2001).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Stomach;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL Submitted (JUL-2002) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [7]
RP TISSUE SPECIFICITY.
RX PubMed=25680266; DOI=10.1016/j.neurobiolaging.2015.01.001;
RA Jensen M.M., Arvaniti M., Mikkelsen J.D., Michalski D., Pinborg L.H.,
RA Haertig W., Thomsen M.S.;
RT "Prostate stem cell antigen interacts with nicotinic acetylcholine
RT receptors and is affected in Alzheimer's disease.";
RL Neurobiol. Aging 36:1629-1638(2015).
CC -!- FUNCTION: May be involved in the regulation of cell proliferation.
CC {ECO:0000250}.
CC -!- FUNCTION: May act as a modulator of nicotinic acetylcholine receptors
CC (nAChRs) activity. In vitro inhibits nicotine-induced signaling
CC probably implicating alpha-3:beta-2- or alpha-7-containing nAChRs.
CC {ECO:0000250|UniProtKB:O43653}.
CC -!- SUBUNIT: Interacts with CHRNA4. {ECO:0000250|UniProtKB:O43653}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:O43653};
CC Lipid-anchor, GPI-anchor {ECO:0000250|UniProtKB:O43653}.
CC -!- TISSUE SPECIFICITY: Predominantly expressed in prostate. Also found in
CC spleen, liver, lung, prostate, kidney and testis. Expressed in brain
CC cortex; expression is increased in transgenic mouse model of Alzheimer
CC disease (at protein level). {ECO:0000269|PubMed:25680266}.
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DR EMBL; AF319173; AAK84073.1; -; mRNA.
DR EMBL; AK008851; BAB25929.1; -; mRNA.
DR EMBL; AC118022; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH466545; EDL29439.1; -; Genomic_DNA.
DR EMBL; BC110462; AAI10463.1; -; mRNA.
DR CCDS; CCDS27526.1; -.
DR RefSeq; NP_082492.1; NM_028216.2.
DR AlphaFoldDB; P57096; -.
DR SMR; P57096; -.
DR STRING; 10090.ENSMUSP00000023265; -.
DR GlyGen; P57096; 1 site.
DR PhosphoSitePlus; P57096; -.
DR PaxDb; P57096; -.
DR PeptideAtlas; P57096; -.
DR PRIDE; P57096; -.
DR ProteomicsDB; 291657; -.
DR Antibodypedia; 7385; 361 antibodies from 38 providers.
DR Ensembl; ENSMUST00000023265; ENSMUSP00000023265; ENSMUSG00000022598.
DR GeneID; 72373; -.
DR KEGG; mmu:72373; -.
DR UCSC; uc007wfr.2; mouse.
DR CTD; 8000; -.
DR MGI; MGI:1919623; Psca.
DR VEuPathDB; HostDB:ENSMUSG00000022598; -.
DR eggNOG; ENOG502SCWD; Eukaryota.
DR GeneTree; ENSGT00940000153378; -.
DR InParanoid; P57096; -.
DR OMA; ISKGCTS; -.
DR OrthoDB; 1536888at2759; -.
DR PhylomeDB; P57096; -.
DR TreeFam; TF336080; -.
DR Reactome; R-MMU-163125; Post-translational modification: synthesis of GPI-anchored proteins.
DR BioGRID-ORCS; 72373; 2 hits in 71 CRISPR screens.
DR ChiTaRS; Psca; mouse.
DR PRO; PR:P57096; -.
DR Proteomes; UP000000589; Chromosome 15.
DR RNAct; P57096; protein.
DR Bgee; ENSMUSG00000022598; Expressed in epithelium of stomach and 62 other tissues.
DR GO; GO:0031225; C:anchored component of membrane; IBA:GO_Central.
DR GO; GO:0016020; C:membrane; TAS:MGI.
DR GO; GO:0005886; C:plasma membrane; ISO:MGI.
DR GO; GO:0033130; F:acetylcholine receptor binding; ISO:MGI.
DR GO; GO:0070373; P:negative regulation of ERK1 and ERK2 cascade; ISO:MGI.
DR GO; GO:0099601; P:regulation of neurotransmitter receptor activity; ISO:MGI.
DR CDD; cd00117; LU; 1.
DR Gene3D; 2.10.60.10; -; 1.
DR InterPro; IPR018363; CD59_antigen_CS.
DR InterPro; IPR016054; LY6_UPA_recep-like.
DR InterPro; IPR045860; Snake_toxin-like_sf.
DR Pfam; PF00021; UPAR_LY6; 1.
DR SMART; SM00134; LU; 1.
DR SUPFAM; SSF57302; SSF57302; 1.
DR PROSITE; PS00983; LY6_UPAR; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Disulfide bond; Glycoprotein; GPI-anchor; Lipoprotein;
KW Membrane; Reference proteome; Signal.
FT SIGNAL 1..20
FT /evidence="ECO:0000250|UniProtKB:O43653"
FT CHAIN 21..95
FT /note="Prostate stem cell antigen"
FT /id="PRO_0000036164"
FT PROPEP 96..123
FT /note="Removed in mature form"
FT /evidence="ECO:0000250"
FT /id="PRO_0000036165"
FT DOMAIN 21..95
FT /note="UPAR/Ly6"
FT LIPID 95
FT /note="GPI-anchor amidated asparagine"
FT /evidence="ECO:0000250"
FT CARBOHYD 40
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT DISULFID 23..48
FT /evidence="ECO:0000250|UniProtKB:P0DP57"
FT DISULFID 26..35
FT /evidence="ECO:0000250|UniProtKB:P0DP57"
FT DISULFID 41..66
FT /evidence="ECO:0000250|UniProtKB:P0DP57"
FT DISULFID 70..86
FT /evidence="ECO:0000250|UniProtKB:P0DP57"
FT DISULFID 87..92
FT /evidence="ECO:0000250|UniProtKB:P0DP57"
FT CONFLICT 5
FT /note="F -> L (in Ref. 1)"
SQ SEQUENCE 123 AA; 13478 MW; 67A8566F3D30797A CRC64;
MKTVFFLLLA TYLALHPGAA LQCYSCTAQM NNRDCLNVQN CSLDQHSCFT SRIRAIGLVT
VISKGCSSQC EDDSENYYLG KKNITCCYSD LCNVNGAHTL KPPTTLGLLT VLCSLLLWGS
SRL
