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PSCE_PSEAE
ID   PSCE_PSEAE              Reviewed;          67 AA.
AC   Q9I317; P95433;
DT   19-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   03-AUG-2022, entry version 97.
DE   RecName: Full=Type III export protein PscE;
DE   AltName: Full=Pseudomonas secretion protein E;
GN   Name=pscE; OrderedLocusNames=PA1718;
OS   Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS   14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=208964;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=388;
RX   PubMed=8971719; DOI=10.1046/j.1365-2958.1996.01554.x;
RA   Yahr T.L., Goranson J., Frank D.W.;
RT   "Exoenzyme S of Pseudomonas aeruginosa is secreted by a type III pathway.";
RL   Mol. Microbiol. 22:991-1003(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC   PRS 101 / PAO1;
RX   PubMed=10984043; DOI=10.1038/35023079;
RA   Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA   Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA   Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA   Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA   Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA   Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT   "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT   pathogen.";
RL   Nature 406:959-964(2000).
RN   [3]
RP   FUNCTION, SUBUNIT, AND SUBCELLULAR LOCATION.
RC   STRAIN=CHA;
RX   PubMed=16115870; DOI=10.1074/jbc.m508089200;
RA   Quinaud M., Chabert J., Faudry E., Neumann E., Lemaire D., Pastor A.,
RA   Elsen S., Dessen A., Attree I.;
RT   "The PscE-PscF-PscG complex controls type III secretion needle biogenesis
RT   in Pseudomonas aeruginosa.";
RL   J. Biol. Chem. 280:36293-36300(2005).
RN   [4]
RP   FUNCTION, INTERACTION WITH PSCG, MUTAGENESIS OF LEU-20; LEU-24 AND GLY-57,
RP   AND DISRUPTION PHENOTYPE.
RX   PubMed=20494986; DOI=10.1128/jb.00117-10;
RA   Ple S., Job V., Dessen A., Attree I.;
RT   "Cochaperone interactions in export of the type III needle component PscF
RT   of Pseudomonas aeruginosa.";
RL   J. Bacteriol. 192:3801-3808(2010).
RN   [5]
RP   X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS), AND INTERACTION WITH PSCF AND PSCG.
RX   PubMed=17470796; DOI=10.1073/pnas.0610098104;
RA   Quinaud M., Ple S., Job V., Contreras-Martel C., Simorre J.P., Attree I.,
RA   Dessen A.;
RT   "Structure of the heterotrimeric complex that regulates type III secretion
RT   needle formation.";
RL   Proc. Natl. Acad. Sci. U.S.A. 104:7803-7808(2007).
CC   -!- FUNCTION: Chaperone of the type III secretion (T3S) injectisome that
CC       translocates effector toxins into host cells, facilitating the
CC       establishment and dissemination of infection. Prevents premature
CC       polymerization of PscF, along with PscG, within the cytoplasm
CC       (PubMed:20494986). Required for type III secretion needle assembly.
CC       Also required for cytotoxicity by influencing PscF levels.
CC       {ECO:0000269|PubMed:16115870, ECO:0000269|PubMed:20494986}.
CC   -!- SUBUNIT: Forms a stable ternary complex with PscF and PscG within the
CC       cytoplasm. Co-stabilized by PscG. {ECO:0000269|PubMed:16115870,
CC       ECO:0000269|PubMed:20494986}.
CC   -!- INTERACTION:
CC       Q9I317; P95435: pscG; NbExp=3; IntAct=EBI-6411628, EBI-6411621;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:16115870}.
CC   -!- DISRUPTION PHENOTYPE: Deletion mutant looses the capacity to lyse
CC       macrophages, indicating the absence of a functional T3SS.
CC       {ECO:0000269|PubMed:20494986}.
CC   -!- SIMILARITY: Belongs to the YscE family. {ECO:0000305}.
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DR   EMBL; U56077; AAC44776.1; -; Genomic_DNA.
DR   EMBL; AE004091; AAG05107.1; -; Genomic_DNA.
DR   PIR; F83430; F83430.
DR   RefSeq; NP_250409.1; NC_002516.2.
DR   RefSeq; WP_003115377.1; NZ_QZGE01000003.1.
DR   PDB; 2UWJ; X-ray; 2.00 A; E=1-67.
DR   PDBsum; 2UWJ; -.
DR   AlphaFoldDB; Q9I317; -.
DR   SMR; Q9I317; -.
DR   DIP; DIP-60933N; -.
DR   IntAct; Q9I317; 2.
DR   STRING; 287.DR97_169; -.
DR   PaxDb; Q9I317; -.
DR   DNASU; 881908; -.
DR   EnsemblBacteria; AAG05107; AAG05107; PA1718.
DR   GeneID; 881908; -.
DR   KEGG; pae:PA1718; -.
DR   PseudoCAP; PA1718; -.
DR   HOGENOM; CLU_202959_0_0_6; -.
DR   OMA; GACPEHF; -.
DR   BioCyc; PAER208964:G1FZ6-1749-MON; -.
DR   EvolutionaryTrace; Q9I317; -.
DR   Proteomes; UP000002438; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0030254; P:protein secretion by the type III secretion system; IGC:PseudoCAP.
DR   InterPro; IPR012671; T3SS_scE.
DR   Pfam; PF08988; T3SS_needle_E; 1.
DR   TIGRFAMs; TIGR02501; type_III_yscE; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Coiled coil; Cytoplasm; Reference proteome; Virulence.
FT   CHAIN           1..67
FT                   /note="Type III export protein PscE"
FT                   /id="PRO_0000250212"
FT   COILED          16..37
FT                   /evidence="ECO:0000255"
FT   VARIANT         40
FT                   /note="C -> G (in strain: 388)"
FT   VARIANT         43
FT                   /note="H -> R (in strain: 388)"
FT   MUTAGEN         20
FT                   /note="L->S: About 50% loss of ability to lyse macrophages;
FT                   in association with S-24."
FT                   /evidence="ECO:0000269|PubMed:20494986"
FT   MUTAGEN         24
FT                   /note="L->S: About 50% loss of ability to lyse macrophages;
FT                   in association with S-20."
FT                   /evidence="ECO:0000269|PubMed:20494986"
FT   MUTAGEN         57
FT                   /note="G->D: Complete loss of ability to lyse macrophages."
FT                   /evidence="ECO:0000269|PubMed:20494986"
FT   HELIX           4..9
FT                   /evidence="ECO:0007829|PDB:2UWJ"
FT   STRAND          11..13
FT                   /evidence="ECO:0007829|PDB:2UWJ"
FT   HELIX           15..37
FT                   /evidence="ECO:0007829|PDB:2UWJ"
FT   HELIX           41..65
FT                   /evidence="ECO:0007829|PDB:2UWJ"
SQ   SEQUENCE   67 AA;  7321 MW;  62339AFECA931617 CRC64;
     MMTALETRLS VADGTHAAAL RQRLQAALAE CRRELARGAC PEHFQFLQQQ ARALEGGLGI
     LSQLTED
 
 
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