PSCF_PSEAE
ID PSCF_PSEAE Reviewed; 85 AA.
AC P95434; Q7DCE8;
DT 19-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 3.
DT 03-AUG-2022, entry version 117.
DE RecName: Full=Type III needle protein PscF {ECO:0000303|PubMed:32601072};
DE AltName: Full=Pseudomonas secretion protein F;
GN Name=pscF; OrderedLocusNames=PA1719;
OS Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=208964;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=388;
RX PubMed=8971719; DOI=10.1046/j.1365-2958.1996.01554.x;
RA Yahr T.L., Goranson J., Frank D.W.;
RT "Exoenzyme S of Pseudomonas aeruginosa is secreted by a type III pathway.";
RL Mol. Microbiol. 22:991-1003(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=10984043; DOI=10.1038/35023079;
RA Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT pathogen.";
RL Nature 406:959-964(2000).
RN [3]
RP FUNCTION, AND IDENTIFICATION BY MASS SPECTROMETRY.
RC STRAIN=CHA;
RX PubMed=16239085; DOI=10.1016/j.femsle.2005.09.028;
RA Pastor A., Chabert J., Louwagie M., Garin J., Attree I.;
RT "PscF is a major component of the Pseudomonas aeruginosa type III secretion
RT needle.";
RL FEMS Microbiol. Lett. 253:95-101(2005).
RN [4]
RP FUNCTION, SUBUNIT, MASS SPECTROMETRY, SUBCELLULAR LOCATION, AND INTERACTION
RP WITH PSCE AND PSCG.
RC STRAIN=CHA;
RX PubMed=16115870; DOI=10.1074/jbc.m508089200;
RA Quinaud M., Chabert J., Faudry E., Neumann E., Lemaire D., Pastor A.,
RA Elsen S., Dessen A., Attree I.;
RT "The PscE-PscF-PscG complex controls type III secretion needle biogenesis
RT in Pseudomonas aeruginosa.";
RL J. Biol. Chem. 280:36293-36300(2005).
RN [5]
RP FUNCTION, MUTAGENESIS OF ASP-76, AND SUBCELLULAR LOCATION.
RC STRAIN=CHA;
RX PubMed=31001211; DOI=10.3389/fmicb.2019.00573;
RA Lombardi C., Tolchard J., Bouillot S., Signor L., Gebus C., Liebl D.,
RA Fenel D., Teulon J.M., Brock J., Habenstein B., Pellequer J.L., Faudry E.,
RA Loquet A., Attree I., Dessen A., Job V.;
RT "Structural and Functional Characterization of the Type Three Secretion
RT System (T3SS) Needle of Pseudomonas aeruginosa.";
RL Front. Microbiol. 10:573-573(2019).
RN [6]
RP FUNCTION, AND MUTAGENESIS OF ASP-14 AND GLN-83.
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=32601072; DOI=10.1128/jb.00055-20;
RA Moir D.T., Bowlin N.O., Berube B.J., Yabut J., Mills D.M., Nguyen G.T.,
RA Aron Z.D., Williams J.D., Mecsas J., Hauser A.R., Bowlin T.L.;
RT "A Structure-Function-Inhibition Analysis of the P. aeruginosa Type III
RT Secretion Needle Protein PscF.";
RL J. Bacteriol. 0:0-0(2020).
RN [7]
RP X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS) OF 55-85, AND INTERACTION WITH PSCE
RP AND PSCG.
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=17470796; DOI=10.1073/pnas.0610098104;
RA Quinaud M., Ple S., Job V., Contreras-Martel C., Simorre J.P., Attree I.,
RA Dessen A.;
RT "Structure of the heterotrimeric complex that regulates type III secretion
RT needle formation.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:7803-7808(2007).
CC -!- FUNCTION: Major component of the type III secretion needle structure
CC that plays an essential role in translocation of effector toxins into
CC host cells, facilitating the establishment and dissemination of
CC infection. {ECO:0000269|PubMed:16115870, ECO:0000269|PubMed:16239085,
CC ECO:0000269|PubMed:31001211, ECO:0000269|PubMed:32601072}.
CC -!- SUBUNIT: Forms a stable heterotrimeric complex with PscE and PscG in
CC the cytoplasm, blocking it in a monomeric state and preventing its
CC polymerization (PubMed:16115870, PubMed:17470796). Then, forms a
CC polymeric structure at the cell surface (PubMed:16115870).
CC {ECO:0000269|PubMed:16115870, ECO:0000269|PubMed:17470796}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:16115870}. Cell
CC surface {ECO:0000269|PubMed:31001211}. Note=Secreted via type III
CC secretion system (TTSS).
CC -!- DOMAIN: The N-terminal part (1-11) is species-specific and essential
CC for export/assembly and the function of needle.
CC -!- MASS SPECTROMETRY: Mass=9153; Method=Electrospray;
CC Evidence={ECO:0000269|PubMed:16115870};
CC -!- SIMILARITY: Belongs to the MxiH/PrgI/YscF family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; U56077; AAC44777.1; -; Genomic_DNA.
DR EMBL; AE004091; AAG05108.1; -; Genomic_DNA.
DR PIR; G83430; G83430.
DR RefSeq; NP_250410.1; NC_002516.2.
DR RefSeq; WP_003087729.1; NZ_QZGE01000003.1.
DR PDB; 2UWJ; X-ray; 2.00 A; F=55-85.
DR PDBsum; 2UWJ; -.
DR AlphaFoldDB; P95434; -.
DR SMR; P95434; -.
DR DIP; DIP-60934N; -.
DR IntAct; P95434; 1.
DR STRING; 287.DR97_168; -.
DR PaxDb; P95434; -.
DR PRIDE; P95434; -.
DR DNASU; 879634; -.
DR EnsemblBacteria; AAG05108; AAG05108; PA1719.
DR GeneID; 879634; -.
DR KEGG; pae:PA1719; -.
DR PATRIC; fig|208964.12.peg.1781; -.
DR PseudoCAP; PA1719; -.
DR HOGENOM; CLU_187681_0_0_6; -.
DR OMA; QHTINKW; -.
DR BioCyc; PAER208964:G1FZ6-1750-MON; -.
DR EvolutionaryTrace; P95434; -.
DR Proteomes; UP000002438; Chromosome.
DR GO; GO:0009986; C:cell surface; IEA:UniProtKB-SubCell.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0030257; C:type III protein secretion system complex; IDA:PseudoCAP.
DR GO; GO:0030254; P:protein secretion by the type III secretion system; IDA:PseudoCAP.
DR InterPro; IPR021123; T3SS_needle-like.
DR InterPro; IPR037203; T3SS_needle-like_sf.
DR InterPro; IPR011841; T3SS_needle_YscF.
DR Pfam; PF09392; T3SS_needle_F; 1.
DR SUPFAM; SSF140129; SSF140129; 1.
DR TIGRFAMs; TIGR02105; III_needle; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Coiled coil; Reference proteome; Secreted; Virulence.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000305"
FT CHAIN 2..85
FT /note="Type III needle protein PscF"
FT /id="PRO_0000249600"
FT COILED 13..41
FT /evidence="ECO:0000255"
FT MUTAGEN 14
FT /note="D->V: Shows a constitutive secretion phenotype."
FT /evidence="ECO:0000269|PubMed:32601072"
FT MUTAGEN 76
FT /note="D->V: Defect in the assembly of the needle on the
FT bacterial surface."
FT /evidence="ECO:0000269|PubMed:31001211"
FT MUTAGEN 83
FT /note="Q->H: Shows a constitutive secretion phenotype."
FT /evidence="ECO:0000269|PubMed:32601072"
FT HELIX 61..63
FT /evidence="ECO:0007829|PDB:2UWJ"
FT HELIX 68..82
FT /evidence="ECO:0007829|PDB:2UWJ"
SQ SEQUENCE 85 AA; 9285 MW; 32177F58EB7E37C4 CRC64;
MAQIFNPNPG NTLDTVANAL KEQANAANKD VNDAIKALQG TDNADNPALL AELQHKINKW
SVIYNINSTV TRALRDLMQG ILQKI
