ID   PSCI_PSEAE              Reviewed;         112 AA.
AC   Q9I315;
DT   07-OCT-2020, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   03-AUG-2022, entry version 90.
DE   RecName: Full=Type III inner-rod protein PscI {ECO:0000303|PubMed:25614137};
GN   Name=pscI; OrderedLocusNames=PA1722;
OS   Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS   14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=208964;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC   PRS 101 / PAO1;
RX   PubMed=10984043; DOI=10.1038/35023079;
RA   Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA   Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA   Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA   Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA   Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA   Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT   "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT   pathogen.";
RL   Nature 406:959-964(2000).
RN   [2]
RP   FUNCTION, SUBUNIT, DISRUPTION PHENOTYPE, AND MUTAGENESIS OF LEU-84; GLU-91;
RP   GLU-92; VAL-105 AND LEU-108.
RC   STRAIN=CHA;
RX   PubMed=25614137; DOI=10.1111/mmi.12947;
RA   Monlezun L., Liebl D., Fenel D., Grandjean T., Berry A., Schoehn G.,
RA   Dessein R., Faudry E., Attree I.;
RT   "PscI is a type III secretion needle anchoring protein with in vitro
RT   polymerization capacities.";
RL   Mol. Microbiol. 96:419-436(2015).
CC   -!- FUNCTION: Component of the type III secretion (T3S) injectisome that
CC       translocates effector toxins into host cells, facilitating the
CC       establishment and dissemination of infection. Polymerizes into flexible
CC       and regularly twisted fibrils and plays an essential role in needle
CC       assembly. {ECO:0000269|PubMed:25614137}.
CC   -!- SUBUNIT: Homomultimer (through its C-terminal region).
CC       {ECO:0000269|PubMed:25614137}.
CC   -!- DISRUPTION PHENOTYPE: Deletion mutants display a reduced cytotoxicity
CC       toward macrophages. {ECO:0000269|PubMed:25614137}.
CC   -!- SIMILARITY: Belongs to the YscI/HrpB family. {ECO:0000305}.
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DR   EMBL; AE004091; AAG05111.1; -; Genomic_DNA.
DR   PIR; B83431; B83431.
DR   RefSeq; NP_250413.1; NC_002516.2.
DR   RefSeq; WP_010895582.1; NZ_QZGE01000003.1.
DR   AlphaFoldDB; Q9I315; -.
DR   SMR; Q9I315; -.
DR   STRING; 287.DR97_165; -.
DR   PaxDb; Q9I315; -.
DR   EnsemblBacteria; AAG05111; AAG05111; PA1722.
DR   GeneID; 879567; -.
DR   KEGG; pae:PA1722; -.
DR   PATRIC; fig|208964.12.peg.1784; -.
DR   PseudoCAP; PA1722; -.
DR   HOGENOM; CLU_171571_0_0_6; -.
DR   OMA; NTQYTEV; -.
DR   BioCyc; PAER208964:G1FZ6-1753-MON; -.
DR   Proteomes; UP000002438; Chromosome.
DR   GO; GO:0030254; P:protein secretion by the type III secretion system; IGC:PseudoCAP.
DR   InterPro; IPR012670; T3SS_YscI/HrpB.
DR   Pfam; PF17001; T3SS_basalb_I; 1.
DR   TIGRFAMs; TIGR02497; yscI_hrpB_dom; 1.
PE   1: Evidence at protein level;
KW   Reference proteome.
FT   CHAIN           1..112
FT                   /note="Type III inner-rod protein PscI"
FT                   /id="PRO_0000451078"
FT   MUTAGEN         84
FT                   /note="L->A: Complete loss of needle assembly."
FT                   /evidence="ECO:0000269|PubMed:25614137"
FT   MUTAGEN         91
FT                   /note="E->K: Complete loss of needle assembly."
FT                   /evidence="ECO:0000269|PubMed:25614137"
FT   MUTAGEN         92
FT                   /note="E->K: Complete loss of needle assembly."
FT                   /evidence="ECO:0000269|PubMed:25614137"
FT   MUTAGEN         105
FT                   /note="V->K: Complete loss of needle assembly."
FT                   /evidence="ECO:0000269|PubMed:25614137"
FT   MUTAGEN         108
FT                   /note="L->K: Complete loss of needle assembly."
FT                   /evidence="ECO:0000269|PubMed:25614137"
SQ   SEQUENCE   112 AA;  12173 MW;  0A3AEB1B416BFC7A CRC64;
     MDISRMGAQA QITSLEELSG GPAGAAHVAE FERAMGGAGS LGGDLLSELG QIRERFSQAK
     QELQMELSTP GDDPNSLMQM QWSLMRITMQ EELIAKTVGR MSQNVETLMK TQ