PSD2_MOUSE
ID PSD2_MOUSE Reviewed; 770 AA.
AC Q6P1I6; Q6NZP0; Q6PHQ7; Q8BHR9; Q9D3B8;
DT 20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 142.
DE RecName: Full=PH and SEC7 domain-containing protein 2;
DE AltName: Full=Exchange factor for ADP-ribosylation factor guanine nucleotide factor 6 C;
DE Short=Exchange factor for ARF6 C;
DE AltName: Full=Pleckstrin homology and SEC7 domain-containing protein 2;
GN Name=Psd2; Synonyms=Efa6c {ECO:0000303|PubMed:23603394};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3), AND NUCLEOTIDE SEQUENCE
RP [LARGE SCALE MRNA] OF 373-770 (ISOFORM 1).
RC STRAIN=C57BL/6J; TISSUE=Corpora quadrigemina, and Medulla oblongata;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 3).
RC STRAIN=C57BL/6J; TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-188, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [4]
RP SUBCELLULAR LOCATION.
RX PubMed=23603394; DOI=10.1016/j.febslet.2013.03.042;
RA Ueda T., Hanai A., Takei T., Kubo K., Ohgi M., Sakagami H., Takahashi S.,
RA Shin H.W., Nakayama K.;
RT "EFA6 activates Arf6 and participates in its targeting to the Flemming body
RT during cytokinesis.";
RL FEBS Lett. 587:1617-1623(2013).
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:23603394};
CC Single-pass membrane protein {ECO:0000305}. Cell projection, ruffle
CC membrane {ECO:0000269|PubMed:23603394}. Cleavage furrow
CC {ECO:0000269|PubMed:23603394}. Note=In interphase associated with the
CC plasma membrane, in particular with membrane ruffling regions. In cells
CC undergoing cytokinesis, transiently found around the ingressing
CC cleavage furrow. Not detected at the midbody ring/Flemming body.
CC {ECO:0000269|PubMed:23603394}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q6P1I6-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q6P1I6-2; Sequence=VSP_033640;
CC Name=3;
CC IsoId=Q6P1I6-3; Sequence=VSP_033639;
CC -!- SIMILARITY: Belongs to the PSD family. {ECO:0000305}.
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DR EMBL; AK018116; BAB31078.2; -; mRNA.
DR EMBL; AK045529; BAC32407.1; -; mRNA.
DR EMBL; BC056437; AAH56437.1; -; mRNA.
DR EMBL; BC062930; AAH62930.1; -; mRNA.
DR EMBL; BC065053; AAH65053.1; -; mRNA.
DR EMBL; BC065055; AAH65055.1; -; mRNA.
DR EMBL; BC066026; AAH66026.1; -; mRNA.
DR EMBL; BC066036; AAH66036.1; -; mRNA.
DR CCDS; CCDS37767.1; -. [Q6P1I6-1]
DR CCDS; CCDS79612.1; -. [Q6P1I6-2]
DR RefSeq; NP_001276531.1; NM_001289602.1. [Q6P1I6-2]
DR RefSeq; NP_082983.3; NM_028707.4. [Q6P1I6-1]
DR RefSeq; XP_017173484.1; XM_017317995.1.
DR AlphaFoldDB; Q6P1I6; -.
DR SMR; Q6P1I6; -.
DR BioGRID; 216414; 1.
DR IntAct; Q6P1I6; 1.
DR STRING; 10090.ENSMUSP00000111381; -.
DR iPTMnet; Q6P1I6; -.
DR PhosphoSitePlus; Q6P1I6; -.
DR MaxQB; Q6P1I6; -.
DR PaxDb; Q6P1I6; -.
DR PeptideAtlas; Q6P1I6; -.
DR PRIDE; Q6P1I6; -.
DR ProteomicsDB; 301860; -. [Q6P1I6-1]
DR ProteomicsDB; 301861; -. [Q6P1I6-2]
DR ProteomicsDB; 301862; -. [Q6P1I6-3]
DR Antibodypedia; 45396; 28 antibodies from 16 providers.
DR DNASU; 74002; -.
DR Ensembl; ENSMUST00000115716; ENSMUSP00000111381; ENSMUSG00000024347. [Q6P1I6-1]
DR Ensembl; ENSMUST00000175734; ENSMUSP00000135795; ENSMUSG00000024347. [Q6P1I6-3]
DR Ensembl; ENSMUST00000176873; ENSMUSP00000135616; ENSMUSG00000024347. [Q6P1I6-3]
DR Ensembl; ENSMUST00000177432; ENSMUSP00000135431; ENSMUSG00000024347. [Q6P1I6-2]
DR GeneID; 74002; -.
DR KEGG; mmu:74002; -.
DR UCSC; uc008ena.3; mouse. [Q6P1I6-1]
DR UCSC; uc008enb.3; mouse. [Q6P1I6-2]
DR CTD; 84249; -.
DR MGI; MGI:1921252; Psd2.
DR VEuPathDB; HostDB:ENSMUSG00000024347; -.
DR eggNOG; KOG0932; Eukaryota.
DR GeneTree; ENSGT00940000159674; -.
DR HOGENOM; CLU_011021_4_0_1; -.
DR InParanoid; Q6P1I6; -.
DR OMA; PKETHDK; -.
DR OrthoDB; 301851at2759; -.
DR PhylomeDB; Q6P1I6; -.
DR TreeFam; TF319755; -.
DR BioGRID-ORCS; 74002; 2 hits in 71 CRISPR screens.
DR ChiTaRS; Psd2; mouse.
DR PRO; PR:Q6P1I6; -.
DR Proteomes; UP000000589; Chromosome 18.
DR RNAct; Q6P1I6; protein.
DR Bgee; ENSMUSG00000024347; Expressed in cerebellum lobe and 107 other tissues.
DR ExpressionAtlas; Q6P1I6; baseline and differential.
DR Genevisible; Q6P1I6; MM.
DR GO; GO:0032154; C:cleavage furrow; IDA:UniProtKB.
DR GO; GO:0030425; C:dendrite; IDA:MGI.
DR GO; GO:0098978; C:glutamatergic synapse; IDA:SynGO.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0043025; C:neuronal cell body; IDA:MGI.
DR GO; GO:0098794; C:postsynapse; IDA:SynGO.
DR GO; GO:0032587; C:ruffle membrane; IDA:UniProtKB.
DR GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; IEA:InterPro.
DR GO; GO:0005543; F:phospholipid binding; IEA:InterPro.
DR GO; GO:0032012; P:regulation of ARF protein signal transduction; IEA:InterPro.
DR CDD; cd00171; Sec7; 1.
DR Gene3D; 1.10.1000.11; -; 1.
DR Gene3D; 2.30.29.30; -; 1.
DR InterPro; IPR011993; PH-like_dom_sf.
DR InterPro; IPR041681; PH_9.
DR InterPro; IPR001605; PH_dom-spectrin-type.
DR InterPro; IPR001849; PH_domain.
DR InterPro; IPR023394; Sec7_C_sf.
DR InterPro; IPR000904; Sec7_dom.
DR InterPro; IPR035999; Sec7_dom_sf.
DR Pfam; PF15410; PH_9; 1.
DR Pfam; PF01369; Sec7; 1.
DR PRINTS; PR00683; SPECTRINPH.
DR SMART; SM00233; PH; 1.
DR SMART; SM00222; Sec7; 1.
DR SUPFAM; SSF48425; SSF48425; 1.
DR PROSITE; PS50003; PH_DOMAIN; 1.
DR PROSITE; PS50190; SEC7; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cell membrane; Cell projection; Coiled coil;
KW Membrane; Phosphoprotein; Reference proteome; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..770
FT /note="PH and SEC7 domain-containing protein 2"
FT /id="PRO_0000334163"
FT TRANSMEM 619..636
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 256..459
FT /note="SEC7"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00189"
FT DOMAIN 509..622
FT /note="PH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00145"
FT REGION 1..65
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 181..304
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 738..770
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 650..677
FT /evidence="ECO:0000255"
FT COMPBIAS 1..27
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 220..241
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 268..295
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 188
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT VAR_SEQ 420
FT /note="H -> HV (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:15489334,
FT ECO:0000303|PubMed:16141072"
FT /id="VSP_033639"
FT VAR_SEQ 526..529
FT /note="GKRT -> A (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_033640"
SQ SEQUENCE 770 AA; 84299 MW; A64A9D0EA9F783F0 CRC64;
MDEEKLPCEL HKEGSATQED HGLEPEEEPG LQNGTAASEG LSSHISGPGG EKTLEGTMEP
VRGPDVALPG LNLSLTNGLA LGQDGNILED SIEFKTWRSG PAEEEDVPGS PCPDAGDPQL
GLDCPGEPDV RDGFSATFEK ILESELLRGT QYSSLDSLDV LSLTDESDSC VSFEAPLTPL
IQQRARDSPE AGAGLGNGDM GPEGDLGATG GCDGELGSPL RRSISSSRSE NVLSHLSLTS
VPNGFHEDGP GGSGGDDEDD EDTDKLLNSA SDTSLKDGLS DSDSELSSSE GLEPGSTDPL
ANGCQGVSEA ARRLARRLYH LEGFQRCDVA RQLGKNNEFS RLVAGEYLSF FDFSGLTLDR
ALRTFLKAFP LMGETQERER VLTHFSRRYC QCNPDDSTSE DGIHTLTCAL MLLNTDLHGH
NIGKKMSCQQ FIANLDQLND GQDFAKDLLK TLYNSIKNEK LEWAIDEDEL RKSLSELVDD
KFGTGTKKVT RILDGGNPFL DVPQALNATT YKHGVLTRKT HADMDGKRTP RGRRGWKKFY
AVLKGTILYL QKDEYRLDKA LSEGDLKNAI RVHHALATRA SDYSKKSNVL KLKTADWRVF
LFQAPSKEEM LSWILRINLV AAIFSAPAFP AAVSSMKKFC RPLLPSCTTR LCQEEQLRSH
ENKLRQVTAE LAEHRCHPLE RGLKSKEAEE YRLKEHYLTF EKSRYETYIH LLAVKIKVGS
DDLERIEARL ATIEGDDPAL RKTHSSPALS LGHGPVTGSK ATKDTSASDT
