ID   AACUB_ASPA1             Reviewed;         734 AA.
AC   A0A1L9WLF2;
DT   29-SEP-2021, integrated into UniProtKB/Swiss-Prot.
DT   15-MAR-2017, sequence version 1.
DT   29-SEP-2021, entry version 22.
DE   RecName: Full=Transcriptional regulator AacuB {ECO:0000303|PubMed:28196534};
DE   AltName: Full=Secalonic acid biosynthesis cluster protein B {ECO:0000303|PubMed:30996871};
GN   Name=AacuB {ECO:0000303|PubMed:30996871}; ORFNames=ASPACDRAFT_33567;
OS   Aspergillus aculeatus (strain ATCC 16872 / CBS 172.66 / WB 5094).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC   Aspergillus subgen. Circumdati.
OX   NCBI_TaxID=690307;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 16872 / CBS 172.66 / WB 5094;
RX   PubMed=28196534; DOI=10.1186/s13059-017-1151-0;
RA   de Vries R.P., Riley R., Wiebenga A., Aguilar-Osorio G., Amillis S.,
RA   Uchima C.A., Anderluh G., Asadollahi M., Askin M., Barry K., Battaglia E.,
RA   Bayram O., Benocci T., Braus-Stromeyer S.A., Caldana C., Canovas D.,
RA   Cerqueira G.C., Chen F., Chen W., Choi C., Clum A., Dos Santos R.A.,
RA   Damasio A.R., Diallinas G., Emri T., Fekete E., Flipphi M., Freyberg S.,
RA   Gallo A., Gournas C., Habgood R., Hainaut M., Harispe M.L., Henrissat B.,
RA   Hilden K.S., Hope R., Hossain A., Karabika E., Karaffa L., Karanyi Z.,
RA   Krasevec N., Kuo A., Kusch H., LaButti K., Lagendijk E.L., Lapidus A.,
RA   Levasseur A., Lindquist E., Lipzen A., Logrieco A.F., MacCabe A.,
RA   Maekelae M.R., Malavazi I., Melin P., Meyer V., Mielnichuk N., Miskei M.,
RA   Molnar A.P., Mule G., Ngan C.Y., Orejas M., Orosz E., Ouedraogo J.P.,
RA   Overkamp K.M., Park H.-S., Perrone G., Piumi F., Punt P.J., Ram A.F.,
RA   Ramon A., Rauscher S., Record E., Riano-Pachon D.M., Robert V., Roehrig J.,
RA   Ruller R., Salamov A., Salih N.S., Samson R.A., Sandor E., Sanguinetti M.,
RA   Schuetze T., Sepcic K., Shelest E., Sherlock G., Sophianopoulou V.,
RA   Squina F.M., Sun H., Susca A., Todd R.B., Tsang A., Unkles S.E.,
RA   van de Wiele N., van Rossen-Uffink D., Oliveira J.V., Vesth T.C.,
RA   Visser J., Yu J.-H., Zhou M., Andersen M.R., Archer D.B., Baker S.E.,
RA   Benoit I., Brakhage A.A., Braus G.H., Fischer R., Frisvad J.C.,
RA   Goldman G.H., Houbraken J., Oakley B., Pocsi I., Scazzocchio C.,
RA   Seiboth B., vanKuyk P.A., Wortman J., Dyer P.S., Grigoriev I.V.;
RT   "Comparative genomics reveals high biological diversity and specific
RT   adaptations in the industrially and medically important fungal genus
RT   Aspergillus.";
RL   Genome Biol. 18:RESEARCH28.1-RESEARCH28.45(2017).
RN   [2]
RP   BIOTECHNOLOGY.
RX   PubMed=28253713; DOI=10.4149/neo_2017_304;
RA   Gao X., Sun H.L., Liu D.S., Zhang J.R., Zhang J., Yan M.M., Pan X.H.;
RT   "Secalonic acid- F inhibited cell growth more effectively than 5-
RT   fluorouracil on hepatocellular carcinoma in vitro and in vivo.";
RL   Neoplasma 64:344-350(2017).
RN   [3]
RP   BIOTECHNOLOGY.
RX   PubMed=29248948; DOI=10.1007/s00284-017-1411-y;
RA   Yodsing N., Lekphrom R., Sangsopha W., Aimi T., Boonlue S.;
RT   "Secondary Metabolites and Their Biological Activity from Aspergillus
RT   aculeatus KKU-CT2.";
RL   Curr. Microbiol. 75:513-518(2018).
RN   [4]
RP   IDENTIFICATION, AND FUNCTION.
RX   PubMed=30996871; DOI=10.1039/c8sc05126g;
RA   Greco C., de Mattos-Shipley K., Bailey A.M., Mulholland N.P., Vincent J.L.,
RA   Willis C.L., Cox R.J., Simpson T.J.;
RT   "Structure revision of cryptosporioptides and determination of the genetic
RT   basis for dimeric xanthone biosynthesis in fungi.";
RL   Chem. Sci. 10:2930-2939(2019).
RN   [5]
RP   BIOTECHNOLOGY.
RX   PubMed=30678274; DOI=10.3390/molecules24030393;
RA   Xie L., Li M., Liu D., Wang X., Wang P., Dai H., Yang W., Liu W., Hu X.,
RA   Zhao M.;
RT   "Secalonic Acid-F, a Novel Mycotoxin, Represses the Progression of
RT   Hepatocellular Carcinoma via MARCH1 Regulation of the PI3K/AKT/beta-catenin
RT   Signaling Pathway.";
RL   Molecules 24:0-0(2019).
RN   [6]
RP   BIOTECHNOLOGY.
RX   PubMed=33015446; DOI=10.1021/acsomega.0c02505;
RA   Farooq S., Qayum A., Nalli Y., Lauro G., Chini M.G., Bifulco G.,
RA   Chaubey A., Singh S.K., Riyaz-Ul-Hassan S., Ali A.;
RT   "Discovery of a Secalonic Acid Derivative from Aspergillus aculeatus, an
RT   Endophyte of Rosa damascena Mill., Triggers Apoptosis in MDA-MB-231 Triple
RT   Negative Breast Cancer Cells.";
RL   ACS Omega 5:24296-24310(2020).
RN   [7]
RP   FUNCTION.
RX   PubMed=33891392; DOI=10.1021/acs.jnatprod.1c00022;
RA   Wei X., Chen X., Chen L., Yan D., Wang W.G., Matsuda Y.;
RT   "Heterologous biosynthesis of tetrahydroxanthone dimers: determination of
RT   key factors for selective or divergent synthesis.";
RL   J. Nat. Prod. 84:1544-1549(2021).
CC   -!- FUNCTION: Transcriptional regulator; part of the gene cluster that
CC       mediates the biosynthesis of the tetrahydroxanthone dimer secalonic
CC       acid D. {ECO:0000269|PubMed:30996871, ECO:0000269|PubMed:33891392}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00227}.
CC   -!- BIOTECHNOLOGY: Secalonic acids show unprecedented anticancer activities
CC       against various human cancer cells and might be interesting for further
CC       derivatization, targeting diseases such as cancer.
CC       {ECO:0000269|PubMed:28253713, ECO:0000269|PubMed:29248948,
CC       ECO:0000269|PubMed:30678274, ECO:0000269|PubMed:33015446}.
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DR   EMBL; KV878984; OJJ96982.1; -; Genomic_DNA.
DR   RefSeq; XP_020053322.1; XM_020199966.1.
DR   EnsemblFungi; OJJ96982; OJJ96982; ASPACDRAFT_33567.
DR   GeneID; 30973780; -.
DR   VEuPathDB; FungiDB:ASPACDRAFT_33567; -.
DR   OrthoDB; 505858at2759; -.
DR   Proteomes; UP000184546; Unassembled WGS sequence.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IEA:InterPro.
DR   GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR   CDD; cd00067; GAL4; 1.
DR   Gene3D; 4.10.240.10; -; 1.
DR   InterPro; IPR007219; Transcription_factor_dom_fun.
DR   InterPro; IPR001138; Zn2-C6_fun-type_DNA-bd.
DR   InterPro; IPR036864; Zn2-C6_fun-type_DNA-bd_sf.
DR   Pfam; PF04082; Fungal_trans; 1.
DR   Pfam; PF00172; Zn_clus; 1.
DR   SMART; SM00906; Fungal_trans; 1.
DR   SMART; SM00066; GAL4; 1.
DR   SUPFAM; SSF57701; SSF57701; 1.
DR   PROSITE; PS50048; ZN2_CY6_FUNGAL_2; 1.
PE   1: Evidence at protein level;
KW   DNA-binding; Metal-binding; Nucleus; Reference proteome; Transcription;
KW   Transcription regulation; Zinc.
FT   CHAIN           1..734
FT                   /note="Transcriptional regulator AacuB"
FT                   /id="PRO_0000453505"
FT   DNA_BIND        26..52
FT                   /note="Zn(2)-C6 fungal-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00227"
FT   REGION          86..122
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        89..122
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   734 AA;  82231 MW;  887F4BED084D82AE CRC64;
     MDTPISASNQ TATVVKPQQP RRVLACVLCQ QRKIKCDRTF PCTNCVRAHV QCEQATRQRR
     RRFPEKELLT RLRLYESLLQ QHNIKFDPLH TPTADHRSAS DDGRDDLPEG AESEGTFGER
     EKPAVKTKSL YEFGSCINSL ISGRLILVNR GDDDGNNGED DEHDTGFLHD TDDVRPAVIQ
     KAWNHTFQGQ NNDHLLFGSP VGTVNLSASH PSHVHIFRLW QVYLDNVNPL LKVTHTPTLQ
     TRIIDAASDI TNISPTLEAL MFSIYCVSLL SLSDEQCRAL FGSAKKELST GYQFACQQAL
     RSCSILRSSD RESLTALYLY LVSIRPDTDP ASLSSLLSVA IRIAQRIGIH NESTYGKCSA
     LETEMRHRLW WSLIIFDNRI CEMSDDKTAS LAPTWDCKVP LNVNDFELQP EMKTPPAPNN
     RPTEMLFAVV RSELADFVRH SAFHLNFTNP SLNTIATRLT DETAQLVSLE RALEEKYLAF
     CNPENALHFM TLWTMRGSLA KSRLLQHYSQ CSNTSVPPTD AQRNTGIAHA LRMLECDTEL
     MTSPLTQGYR WLVHFHFPFP AYIHLLQDLK KRPVEAHADR AWEAMSDNYA VRMMDASQDD
     RPFFIVFSRI VLQAWEAREK MAVAAQLETP PPVPPRMVVD IRDKVMQMTA SFGMDAAAAV
     ESGGVVGVKA GDLDMPMQMD FAAPEMAYGA GGHGATGLEP WGCLDMAGPA AGDVGANQFL
     LNTMEWNALH ARDR