ID   ATL_SHIFL               Reviewed;         129 AA.
AC   P0AFP3; P75707; P77119;
DT   20-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT   20-DEC-2005, sequence version 1.
DT   25-MAY-2022, entry version 92.
DE   RecName: Full=DNA base-flipping protein {ECO:0000250|UniProtKB:P0AFP2};
DE   AltName: Full=Alkyltransferase-like protein ATL {ECO:0000250|UniProtKB:P0AFP2};
GN   Name=atl {ECO:0000250|UniProtKB:P0AFP2}; Synonyms=ybaZ;
GN   OrderedLocusNames=SF0400, S0406;
OS   Shigella flexneri.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Shigella.
OX   NCBI_TaxID=623;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=301 / Serotype 2a;
RX   PubMed=12384590; DOI=10.1093/nar/gkf566;
RA   Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J.,
RA   Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L.,
RA   Xue Y., Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H.,
RA   Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J.;
RT   "Genome sequence of Shigella flexneri 2a: insights into pathogenicity
RT   through comparison with genomes of Escherichia coli K12 and O157.";
RL   Nucleic Acids Res. 30:4432-4441(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700930 / 2457T / Serotype 2a;
RX   PubMed=12704152; DOI=10.1128/iai.71.5.2775-2786.2003;
RA   Wei J., Goldberg M.B., Burland V., Venkatesan M.M., Deng W., Fournier G.,
RA   Mayhew G.F., Plunkett G. III, Rose D.J., Darling A., Mau B., Perna N.T.,
RA   Payne S.M., Runyen-Janecky L.J., Zhou S., Schwartz D.C., Blattner F.R.;
RT   "Complete genome sequence and comparative genomics of Shigella flexneri
RT   serotype 2a strain 2457T.";
RL   Infect. Immun. 71:2775-2786(2003).
CC   -!- FUNCTION: Involved in DNA damage recognition. Binds DNA containing
CC       O(6)-methylguanine and larger O(6)-alkylguanine adducts. Binds to the
CC       damaged base and flips the base out of the DNA duplex into an
CC       extrahelical conformation, which allows processing by repair proteins.
CC       {ECO:0000250|UniProtKB:P0AFP2}.
CC   -!- SIMILARITY: Belongs to the MGMT family. ATL subfamily. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAN42056.2; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC       Sequence=AAP15933.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; AE005674; AAN42056.2; ALT_INIT; Genomic_DNA.
DR   EMBL; AE014073; AAP15933.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; NP_706349.2; NC_004337.2.
DR   RefSeq; WP_001300427.1; NZ_LM651928.1.
DR   AlphaFoldDB; P0AFP3; -.
DR   SMR; P0AFP3; -.
DR   STRING; 198214.SF0400; -.
DR   EnsemblBacteria; AAN42056; AAN42056; SF0400.
DR   EnsemblBacteria; AAP15933; AAP15933; S0406.
DR   GeneID; 1027693; -.
DR   KEGG; sfl:SF0400; -.
DR   KEGG; sfx:S0406; -.
DR   PATRIC; fig|198214.7.peg.459; -.
DR   HOGENOM; CLU_000445_52_5_6; -.
DR   Proteomes; UP000001006; Chromosome.
DR   Proteomes; UP000002673; Chromosome.
DR   GO; GO:0003824; F:catalytic activity; IEA:InterPro.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0006281; P:DNA repair; IEA:InterPro.
DR   CDD; cd06445; ATase; 1.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR014048; MethylDNA_cys_MeTrfase_DNA-bd.
DR   InterPro; IPR036217; MethylDNA_cys_MeTrfase_DNAb.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   Pfam; PF01035; DNA_binding_1; 1.
DR   SUPFAM; SSF46767; SSF46767; 1.
DR   TIGRFAMs; TIGR00589; ogt; 1.
PE   3: Inferred from homology;
KW   DNA damage; DNA-binding; Reference proteome.
FT   CHAIN           1..129
FT                   /note="DNA base-flipping protein"
FT                   /id="PRO_0000139391"
FT   SITE            52
FT                   /note="Required for phosphate rotation/nucleotide flipping"
FT                   /evidence="ECO:0000250|UniProtKB:Q9UTN9"
FT   SITE            66
FT                   /note="Arg finger, required for nucleotide flipping"
FT                   /evidence="ECO:0000250|UniProtKB:Q9UTN9"
SQ   SEQUENCE   129 AA;  14450 MW;  584382A5657919CA CRC64;
     MLVSCAMRLH SGVFPDYAEK LPQEEKMEKE DSFPQRVWQI VAAIPEGYVT TYGDVAKLAG
     SPRAARQVGG VLKRLPEGST LPWHRVVNRH GTISLTGPDL QRQRQALLAE GVMVSGSGQI
     DLQRYRWNY