PSE1_ARATH
ID PSE1_ARATH Reviewed; 259 AA.
AC Q93V51; Q9FNH1;
DT 07-OCT-2020, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 125.
DE RecName: Full=Protein LEAD-SENSITIVE 1 {ECO:0000303|PubMed:27335453};
DE Short=Protein Pb-SENSITIVE 1 {ECO:0000303|PubMed:27335453};
GN Name=PSE1 {ECO:0000303|PubMed:27335453};
GN OrderedLocusNames=At5g06370 {ECO:0000312|Araport:AT5G06370};
GN ORFNames=MHF15.11 {ECO:0000312|EMBL:BAB08959.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9405937; DOI=10.1093/dnares/4.4.291;
RA Kotani H., Nakamura Y., Sato S., Kaneko T., Asamizu E., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. II. Sequence
RT features of the regions of 1,044,062 bp covered by thirteen physically
RT assigned P1 clones.";
RL DNA Res. 4:291-300(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP FUNCTION, DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION, INDUCTION BY LEAD
RP STRESS, AND TISSUE SPECIFICITY.
RC STRAIN=cv. Columbia;
RX PubMed=27335453; DOI=10.1093/jxb/erw251;
RA Fan T., Yang L., Wu X., Ni J., Jiang H., Zhang Q., Fang L., Sheng Y.,
RA Ren Y., Cao S.;
RT "The PSE1 gene modulates lead tolerance in Arabidopsis.";
RL J. Exp. Bot. 67:4685-4695(2016).
CC -!- FUNCTION: Confers tolerance to lead ions (Pb) stress mediated by
CC Pb(NO(3))(2) probably by promoting Pb accumulation leading to
CC subsequent glutathione-dependent phytochelatin (PC) synthesis and
CC related gene expression, including PDR12/ABCG40, GSH1, GSH2, GR1, GR2,
CC PCS1 and PCS2. {ECO:0000269|PubMed:27335453}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:27335453}.
CC -!- TISSUE SPECIFICITY: Highly expressed in inflorescences, siliques and
CC stems, and, to a lower extent, in roots and leaves.
CC {ECO:0000269|PubMed:27335453}.
CC -!- INDUCTION: Induced by lead (Pb) stress (Pb(NO(3))(2)).
CC {ECO:0000269|PubMed:27335453}.
CC -!- DISRUPTION PHENOTYPE: Increased sensitivity to lead ions (Pb) stress
CC mediated by Pb(NO(3))(2) associated with a reduced Pb accumulation
CC leading to decreased phytochelatin (PC) synthesis and related gene
CC expression. Higher sensitivity to hydrogen peroxyde H(2)O(2).
CC {ECO:0000269|PubMed:27335453}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB08959.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AB006700; BAB08959.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002688; AED91007.1; -; Genomic_DNA.
DR EMBL; AF380637; AAK55718.1; -; mRNA.
DR EMBL; AY054134; AAL06795.1; -; mRNA.
DR EMBL; AK226465; BAE98607.1; -; mRNA.
DR RefSeq; NP_568167.1; NM_120720.4.
DR AlphaFoldDB; Q93V51; -.
DR IntAct; Q93V51; 3.
DR STRING; 3702.AT5G06370.1; -.
DR PaxDb; Q93V51; -.
DR PRIDE; Q93V51; -.
DR ProteomicsDB; 187426; -.
DR EnsemblPlants; AT5G06370.1; AT5G06370.1; AT5G06370.
DR GeneID; 830525; -.
DR Gramene; AT5G06370.1; AT5G06370.1; AT5G06370.
DR KEGG; ath:AT5G06370; -.
DR Araport; AT5G06370; -.
DR TAIR; locus:2164260; AT5G06370.
DR eggNOG; ENOG502QUIP; Eukaryota.
DR HOGENOM; CLU_062156_0_0_1; -.
DR InParanoid; Q93V51; -.
DR OMA; VIHFTRH; -.
DR OrthoDB; 1602481at2759; -.
DR PhylomeDB; Q93V51; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q93V51; baseline and differential.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0071284; P:cellular response to lead ion; IMP:TAIR.
DR GO; GO:0015692; P:lead ion transport; IMP:UniProtKB.
DR GO; GO:0046938; P:phytochelatin biosynthetic process; IMP:UniProtKB.
DR GO; GO:0010288; P:response to lead ion; IMP:UniProtKB.
DR InterPro; IPR007053; LRAT_dom.
DR Pfam; PF04970; LRAT; 1.
DR PROSITE; PS51934; LRAT; 1.
PE 2: Evidence at transcript level;
KW Cytoplasm; Reference proteome; Stress response.
FT CHAIN 1..259
FT /note="Protein LEAD-SENSITIVE 1"
FT /id="PRO_0000450776"
FT DOMAIN 20..168
FT /note="LRAT"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01283"
FT ACT_SITE 30
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01283"
FT ACT_SITE 42
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01283"
FT ACT_SITE 152
FT /note="Acyl-thioester intermediate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01283"
SQ SEQUENCE 259 AA; 27847 MW; F6C9F4B2DFF958D1 CRC64;
MGLLSNRIDR SSLKPGDHIY SWRTAYIYAH HGIYVGDDRV IHFTRRGQEV GTGTVLDLIL
VSSGPSRNHT HCPTCVPPNE GHGVVSSCLN CFLAGGVLYR FEYSVNAAHF LVKARGGTCT
LAVADPNEIV VHRAKHLLQN GFGCYDVFKN NCEDFAIYCK TALLVLEGRT MGQSGQAVSI
IGGPIAAVLS TPMRLLTTNV YGMAATAIGV YCASRYATDI GMRADVAKVE AEDLTRRLSS
GLFQVLDPPL AAIALPSTS
