PSEH_HELPY
ID PSEH_HELPY Reviewed; 180 AA.
AC O25094;
DT 05-SEP-2012, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1998, sequence version 1.
DT 03-AUG-2022, entry version 106.
DE RecName: Full=UDP-4-amino-4,6-dideoxy-N-acetyl-beta-L-altrosamine N-acetyltransferase;
DE EC=2.3.1.202;
DE AltName: Full=Pseudaminic acid biosynthesis protein H;
GN Name=pseH; OrderedLocusNames=HP_0327;
OS Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori).
OC Bacteria; Proteobacteria; Epsilonproteobacteria; Campylobacterales;
OC Helicobacteraceae; Helicobacter.
OX NCBI_TaxID=85962;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700392 / 26695;
RX PubMed=9252185; DOI=10.1038/41483;
RA Tomb J.-F., White O., Kerlavage A.R., Clayton R.A., Sutton G.G.,
RA Fleischmann R.D., Ketchum K.A., Klenk H.-P., Gill S.R., Dougherty B.A.,
RA Nelson K.E., Quackenbush J., Zhou L., Kirkness E.F., Peterson S.N.,
RA Loftus B.J., Richardson D.L., Dodson R.J., Khalak H.G., Glodek A.,
RA McKenney K., FitzGerald L.M., Lee N., Adams M.D., Hickey E.K., Berg D.E.,
RA Gocayne J.D., Utterback T.R., Peterson J.D., Kelley J.M., Cotton M.D.,
RA Weidman J.F., Fujii C., Bowman C., Watthey L., Wallin E., Hayes W.S.,
RA Borodovsky M., Karp P.D., Smith H.O., Fraser C.M., Venter J.C.;
RT "The complete genome sequence of the gastric pathogen Helicobacter
RT pylori.";
RL Nature 388:539-547(1997).
RN [2]
RP FUNCTION, CATALYTIC ACTIVITY, AND PATHWAY.
RC STRAIN=ATCC 700392 / 26695;
RX PubMed=16751642; DOI=10.1093/glycob/cwl010;
RA Schoenhofen I.C., McNally D.J., Brisson J.R., Logan S.M.;
RT "Elucidation of the CMP-pseudaminic acid pathway in Helicobacter pylori:
RT synthesis from UDP-N-acetylglucosamine by a single enzymatic reaction.";
RL Glycobiology 16:8C-14C(2006).
CC -!- FUNCTION: Catalyzes the third step in the biosynthesis of pseudaminic
CC acid, a sialic-acid-like sugar that is used to modify flagellin.
CC Mediates N-4 acetylation of UDP-4-amino-4,6-dideoxy-beta-L-AltNAc to
CC form UDP-2,4-diacetamido-2,4,6-trideoxy-beta-L-altropyranose.
CC {ECO:0000269|PubMed:16751642}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=acetyl-CoA + UDP-4-amino-4,6-dideoxy-N-acetyl-beta-L-
CC altrosamine = CoA + H(+) + UDP-2,4-diacetamido-2,4,6-trideoxy-beta-L-
CC altrose; Xref=Rhea:RHEA:34155, ChEBI:CHEBI:15378, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57288, ChEBI:CHEBI:63389, ChEBI:CHEBI:63417;
CC EC=2.3.1.202; Evidence={ECO:0000269|PubMed:16751642};
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DR EMBL; AE000511; AAD07394.1; -; Genomic_DNA.
DR PIR; G64560; G64560.
DR RefSeq; NP_207125.1; NC_000915.1.
DR RefSeq; WP_000742697.1; NC_018939.1.
DR PDB; 4RI1; X-ray; 2.30 A; A/B/C=1-180.
DR PDBsum; 4RI1; -.
DR AlphaFoldDB; O25094; -.
DR SMR; O25094; -.
DR DIP; DIP-3555N; -.
DR IntAct; O25094; 4.
DR MINT; O25094; -.
DR STRING; 85962.C694_01655; -.
DR PaxDb; O25094; -.
DR DNASU; 900098; -.
DR EnsemblBacteria; AAD07394; AAD07394; HP_0327.
DR KEGG; hpy:HP_0327; -.
DR PATRIC; fig|85962.8.peg.341; -.
DR eggNOG; COG1670; Bacteria.
DR OMA; NHYELEG; -.
DR PhylomeDB; O25094; -.
DR BioCyc; MetaCyc:HP0327-MON; -.
DR BRENDA; 2.3.1.202; 2604.
DR Proteomes; UP000000429; Chromosome.
DR GO; GO:0008080; F:N-acetyltransferase activity; IEA:InterPro.
DR InterPro; IPR016181; Acyl_CoA_acyltransferase.
DR InterPro; IPR000182; GNAT_dom.
DR InterPro; IPR020036; PseH.
DR SUPFAM; SSF55729; SSF55729; 1.
DR TIGRFAMs; TIGR03585; PseH; 1.
DR PROSITE; PS51186; GNAT; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Acyltransferase; Reference proteome; Transferase.
FT CHAIN 1..180
FT /note="UDP-4-amino-4,6-dideoxy-N-acetyl-beta-L-altrosamine
FT N-acetyltransferase"
FT /id="PRO_0000418961"
FT DOMAIN 13..169
FT /note="N-acetyltransferase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00532"
FT STRAND 5..7
FT /evidence="ECO:0007829|PDB:4RI1"
FT STRAND 10..14
FT /evidence="ECO:0007829|PDB:4RI1"
FT HELIX 15..17
FT /evidence="ECO:0007829|PDB:4RI1"
FT HELIX 20..31
FT /evidence="ECO:0007829|PDB:4RI1"
FT HELIX 33..36
FT /evidence="ECO:0007829|PDB:4RI1"
FT STRAND 39..42
FT /evidence="ECO:0007829|PDB:4RI1"
FT HELIX 46..56
FT /evidence="ECO:0007829|PDB:4RI1"
FT STRAND 63..69
FT /evidence="ECO:0007829|PDB:4RI1"
FT STRAND 72..83
FT /evidence="ECO:0007829|PDB:4RI1"
FT TURN 84..87
FT /evidence="ECO:0007829|PDB:4RI1"
FT STRAND 88..95
FT /evidence="ECO:0007829|PDB:4RI1"
FT HELIX 103..115
FT /evidence="ECO:0007829|PDB:4RI1"
FT STRAND 121..128
FT /evidence="ECO:0007829|PDB:4RI1"
FT HELIX 132..140
FT /evidence="ECO:0007829|PDB:4RI1"
FT STRAND 144..155
FT /evidence="ECO:0007829|PDB:4RI1"
FT STRAND 158..168
FT /evidence="ECO:0007829|PDB:4RI1"
FT HELIX 169..177
FT /evidence="ECO:0007829|PDB:4RI1"
SQ SEQUENCE 180 AA; 21353 MW; 2565A24C8C770DCA CRC64;
MKKNYSYKNI QAIDFTNLND GEKLLVLEFR NHPNTALWMY STFISLKTHL QFIEDLKNSP
NHRYFLFKEE GVYLGVGSIT KINFFHKHGY LGIYKNPFLK NGGETILKAL EFIAFEEFQL
HSLHLEVMEN NFKAIAFYEK NHYELEGRLK GFISKDKEFI DVLLYYKDKK GYNDQSLLKL
