ID   PSF1_MOUSE              Reviewed;         196 AA.
AC   Q9CZ15;
DT   15-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2001, sequence version 1.
DT   03-AUG-2022, entry version 128.
DE   RecName: Full=DNA replication complex GINS protein PSF1;
DE   AltName: Full=GINS complex subunit 1;
GN   Name=Gins1; Synonyms=Psf1;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=C57BL/6J;
RX   PubMed=16141072; DOI=10.1126/science.1112014;
RA   Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA   Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA   Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA   Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA   Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA   Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA   Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA   Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA   Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA   Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA   Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA   Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA   Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA   Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA   Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA   Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA   Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA   Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA   Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA   Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA   Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA   Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA   Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA   Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA   Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA   van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA   Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA   Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA   Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA   Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA   Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA   Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA   Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA   Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT   "The transcriptional landscape of the mammalian genome.";
RL   Science 309:1559-1563(2005).
RN   [2]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC   TISSUE=Spleen, and Testis;
RX   PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA   Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA   Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT   "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL   Cell 143:1174-1189(2010).
CC   -!- FUNCTION: Required for correct functioning of the GINS complex, a
CC       complex that plays an essential role in the initiation of DNA
CC       replication, and progression of DNA replication forks. GINS complex
CC       seems to bind preferentially to single-stranded DNA.
CC       {ECO:0000250|UniProtKB:Q14691}.
CC   -!- SUBUNIT: Component of the GINS complex which is a heterotetramer of
CC       GINS1, GINS2, GINS3 and GINS4. Forms a stable subcomplex with GINS4.
CC       GINS complex interacts with DNA primase in vitro (By similarity).
CC       Component of the CMG helicase complex, composed of the MCM2-7 complex,
CC       the GINS complex and CDC45 (By similarity).
CC       {ECO:0000250|UniProtKB:Q14691, ECO:0000250|UniProtKB:Q7ZT47}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q7ZT47}.
CC       Chromosome {ECO:0000250|UniProtKB:Q7ZT47}. Note=Associates with
CC       chromatin. {ECO:0000250|UniProtKB:Q7ZT47}.
CC   -!- SIMILARITY: Belongs to the GINS1/PSF1 family. {ECO:0000305}.
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DR   EMBL; AK013116; BAB28658.1; -; mRNA.
DR   CCDS; CCDS50743.1; -.
DR   RefSeq; NP_081290.1; NM_027014.1.
DR   AlphaFoldDB; Q9CZ15; -.
DR   SMR; Q9CZ15; -.
DR   BioGRID; 213326; 1.
DR   ComplexPortal; CPX-4502; GINS complex.
DR   STRING; 10090.ENSMUSP00000028948; -.
DR   iPTMnet; Q9CZ15; -.
DR   PhosphoSitePlus; Q9CZ15; -.
DR   EPD; Q9CZ15; -.
DR   MaxQB; Q9CZ15; -.
DR   PaxDb; Q9CZ15; -.
DR   PeptideAtlas; Q9CZ15; -.
DR   PRIDE; Q9CZ15; -.
DR   ProteomicsDB; 301863; -.
DR   Antibodypedia; 10061; 191 antibodies from 26 providers.
DR   Ensembl; ENSMUST00000028948; ENSMUSP00000028948; ENSMUSG00000027454.
DR   GeneID; 69270; -.
DR   KEGG; mmu:69270; -.
DR   UCSC; uc008mus.2; mouse.
DR   CTD; 9837; -.
DR   MGI; MGI:1916520; Gins1.
DR   VEuPathDB; HostDB:ENSMUSG00000027454; -.
DR   eggNOG; KOG3303; Eukaryota.
DR   GeneTree; ENSGT00390000013968; -.
DR   HOGENOM; CLU_079191_1_1_1; -.
DR   InParanoid; Q9CZ15; -.
DR   OMA; NHLCMRR; -.
DR   OrthoDB; 1249159at2759; -.
DR   PhylomeDB; Q9CZ15; -.
DR   TreeFam; TF312848; -.
DR   Reactome; R-MMU-176974; Unwinding of DNA.
DR   BioGRID-ORCS; 69270; 22 hits in 76 CRISPR screens.
DR   ChiTaRS; Tap1; mouse.
DR   PRO; PR:Q9CZ15; -.
DR   Proteomes; UP000000589; Chromosome 2.
DR   RNAct; Q9CZ15; protein.
DR   Bgee; ENSMUSG00000027454; Expressed in 1st arch mandibular component and 200 other tissues.
DR   Genevisible; Q9CZ15; MM.
DR   GO; GO:0071162; C:CMG complex; ISS:UniProtKB.
DR   GO; GO:0005737; C:cytoplasm; IDA:MGI.
DR   GO; GO:0000811; C:GINS complex; ISO:MGI.
DR   GO; GO:0005634; C:nucleus; IDA:MGI.
DR   GO; GO:0006260; P:DNA replication; IMP:MGI.
DR   GO; GO:1902983; P:DNA strand elongation involved in mitotic DNA replication; IBA:GO_Central.
DR   GO; GO:0006268; P:DNA unwinding involved in DNA replication; ISO:MGI.
DR   GO; GO:0001833; P:inner cell mass cell proliferation; IMP:MGI.
DR   GO; GO:1903934; P:positive regulation of DNA primase activity; ISO:MGI.
DR   GO; GO:1900264; P:positive regulation of DNA-directed DNA polymerase activity; ISO:MGI.
DR   CDD; cd11710; GINS_A_psf1; 1.
DR   InterPro; IPR036224; GINS_bundle-like_dom_sf.
DR   InterPro; IPR005339; GINS_Psf1.
DR   PANTHER; PTHR12914; PTHR12914; 1.
DR   SUPFAM; SSF158573; SSF158573; 1.
PE   1: Evidence at protein level;
KW   Chromosome; DNA replication; Nucleus; Reference proteome.
FT   CHAIN           1..196
FT                   /note="DNA replication complex GINS protein PSF1"
FT                   /id="PRO_0000219036"
SQ   SEQUENCE   196 AA;  22893 MW;  EDA492802EDDEE0D CRC64;
     MFCEKAMELV RELHRAPEGQ LPAFNEDGLR QVLEEMKALY EQNQSDVNEA KSAGRGDLIP
     TVKFRHCALL RNRRCTIAYL YDRLLRIRAL RWEYGSVLPN SLRFHMSAEE TEWFNHYKKS
     LATYMRSLGG DEGLDITQDV KPPKSLYIEV RCLKDYGEFE VDDGTSVLLK KNSQHFLPRW
     KCEQLIRQGV LEHVLS