PSF2_YEAST
ID PSF2_YEAST Reviewed; 213 AA.
AC P40359; D6VWB1;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1995, sequence version 1.
DT 03-AUG-2022, entry version 165.
DE RecName: Full=DNA replication complex GINS protein PSF2;
DE AltName: Full=Partner of Sld five 2;
GN Name=PSF2; OrderedLocusNames=YJL072C; ORFNames=HRF213, J1086;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, IDENTIFICATION IN THE GINS
RP COMPLEX, AND SUBCELLULAR LOCATION.
RX PubMed=12730134; DOI=10.1101/gad.1065903;
RA Takayama Y., Kamimura Y., Okawa M., Muramatsu S., Sugino A., Araki H.;
RT "GINS, a novel multiprotein complex required for chromosomal DNA
RT replication in budding yeast.";
RL Genes Dev. 17:1153-1165(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RA Sor F.J.;
RL Submitted (JUN-1995) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=7762302; DOI=10.1002/yea.320110108;
RA Vandenbol M., Durand P., Dion C., Portetelle D., Hilger F.;
RT "Sequence of a 17.1 kb DNA fragment from chromosome X of Saccharomyces
RT cerevisiae includes the mitochondrial ribosomal protein L8.";
RL Yeast 11:57-60(1995).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8641269; DOI=10.1002/j.1460-2075.1996.tb00557.x;
RA Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C.,
RA Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D.,
RA Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J.,
RA Heumann K., Hilger F., Hollenberg C.P., Huang M.-E., Jacq C.,
RA Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E.,
RA Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T.,
RA Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B.,
RA Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R.,
RA Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N.,
RA To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H.,
RA von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.;
RT "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X.";
RL EMBO J. 15:2031-2049(1996).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND INTERACTION WITH PSF1 AND SLD5.
RX PubMed=14690591; DOI=10.1016/s1097-2765(03)00476-3;
RA Hazbun T.R., Malmstroem L., Anderson S., Graczyk B.J., Fox B., Riffle M.,
RA Sundin B.A., Aranda J.D., McDonald W.H., Chiu C.-H., Snydsman B.E.,
RA Bradley P., Muller E.G.D., Fields S., Baker D., Yates J.R. III, Davis T.N.;
RT "Assigning function to yeast proteins by integration of technologies.";
RL Mol. Cell 12:1353-1365(2003).
RN [6]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [7]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
CC -!- FUNCTION: Functions as part of the GINS complex which plays an
CC essential role in the initiation of DNA replication by binding to DNA
CC replication origins and facilitating the assembly of the DNA
CC replication machinery. {ECO:0000269|PubMed:12730134}.
CC -!- SUBUNIT: Component of the GINS complex which is a heterotetramer of
CC SLD5, PSF1, PSF2 and PSF3. Interacts with PSF1 and SLD5.
CC {ECO:0000269|PubMed:12730134, ECO:0000269|PubMed:14690591}.
CC -!- INTERACTION:
CC P40359; Q12488: PSF1; NbExp=5; IntAct=EBI-25936, EBI-22066;
CC P40359; Q12146: PSF3; NbExp=5; IntAct=EBI-25936, EBI-30392;
CC P40359; Q03406: SLD5; NbExp=7; IntAct=EBI-25936, EBI-37437;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:12730134}.
CC -!- SIMILARITY: Belongs to the GINS2/PSF2 family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; X88851; CAA61311.1; -; Genomic_DNA.
DR EMBL; Z34288; CAA84050.1; -; Genomic_DNA.
DR EMBL; Z49347; CAA89364.1; -; Genomic_DNA.
DR EMBL; AY558248; AAS56574.1; -; Genomic_DNA.
DR EMBL; BK006943; DAA08727.1; -; Genomic_DNA.
DR PIR; S50799; S50799.
DR RefSeq; NP_012463.1; NM_001181505.1.
DR PDB; 3JC5; EM; 4.70 A; B=1-213.
DR PDB; 3JC6; EM; 3.70 A; B=1-213.
DR PDB; 3JC7; EM; 4.80 A; B=1-213.
DR PDB; 5U8S; EM; 6.10 A; B=1-213.
DR PDB; 5U8T; EM; 4.90 A; B=1-213.
DR PDB; 6HV9; EM; 4.98 A; D=1-213.
DR PDB; 6PTJ; EM; 3.80 A; B=1-213.
DR PDB; 6PTN; EM; 5.80 A; B/b=1-213.
DR PDB; 6PTO; EM; 7.00 A; B/b/o=1-213.
DR PDB; 6SKL; EM; 3.70 A; B=1-213.
DR PDB; 6U0M; EM; 3.90 A; B=3-200.
DR PDB; 7PMN; EM; 3.20 A; B=1-213.
DR PDBsum; 3JC5; -.
DR PDBsum; 3JC6; -.
DR PDBsum; 3JC7; -.
DR PDBsum; 5U8S; -.
DR PDBsum; 5U8T; -.
DR PDBsum; 6HV9; -.
DR PDBsum; 6PTJ; -.
DR PDBsum; 6PTN; -.
DR PDBsum; 6PTO; -.
DR PDBsum; 6SKL; -.
DR PDBsum; 6U0M; -.
DR PDBsum; 7PMN; -.
DR AlphaFoldDB; P40359; -.
DR SMR; P40359; -.
DR BioGRID; 33683; 199.
DR ComplexPortal; CPX-1641; GINS complex.
DR DIP; DIP-3858N; -.
DR IntAct; P40359; 7.
DR MINT; P40359; -.
DR STRING; 4932.YJL072C; -.
DR MaxQB; P40359; -.
DR PaxDb; P40359; -.
DR PRIDE; P40359; -.
DR EnsemblFungi; YJL072C_mRNA; YJL072C; YJL072C.
DR GeneID; 853373; -.
DR KEGG; sce:YJL072C; -.
DR SGD; S000003608; PSF2.
DR VEuPathDB; FungiDB:YJL072C; -.
DR eggNOG; KOG4071; Eukaryota.
DR GeneTree; ENSGT00390000007838; -.
DR HOGENOM; CLU_078274_1_1_1; -.
DR InParanoid; P40359; -.
DR OMA; PYHWLEL; -.
DR BioCyc; YEAST:G3O-31530-MON; -.
DR Reactome; R-SCE-176974; Unwinding of DNA.
DR PRO; PR:P40359; -.
DR Proteomes; UP000002311; Chromosome X.
DR RNAct; P40359; protein.
DR GO; GO:0071162; C:CMG complex; IDA:SGD.
DR GO; GO:0031261; C:DNA replication preinitiation complex; IPI:SGD.
DR GO; GO:0000811; C:GINS complex; IPI:SGD.
DR GO; GO:0043596; C:nuclear replication fork; IDA:SGD.
DR GO; GO:0005634; C:nucleus; IDA:SGD.
DR GO; GO:0031298; C:replication fork protection complex; IDA:SGD.
DR GO; GO:0006268; P:DNA unwinding involved in DNA replication; IDA:ComplexPortal.
DR GO; GO:0006261; P:DNA-templated DNA replication; IMP:SGD.
DR GO; GO:0000727; P:double-strand break repair via break-induced replication; IMP:SGD.
DR GO; GO:1903934; P:positive regulation of DNA primase activity; IC:ComplexPortal.
DR GO; GO:1900264; P:positive regulation of DNA-directed DNA polymerase activity; IC:ComplexPortal.
DR InterPro; IPR021151; GINS_A.
DR InterPro; IPR036224; GINS_bundle-like_dom_sf.
DR InterPro; IPR007257; GINS_Psf2.
DR PANTHER; PTHR12772; PTHR12772; 1.
DR Pfam; PF05916; Sld5; 1.
DR PIRSF; PIRSF028998; GINS_Psf2_subgr; 1.
DR SUPFAM; SSF158573; SSF158573; 1.
PE 1: Evidence at protein level;
KW 3D-structure; DNA replication; Nucleus; Reference proteome.
FT CHAIN 1..213
FT /note="DNA replication complex GINS protein PSF2"
FT /id="PRO_0000194822"
SQ SEQUENCE 213 AA; 25061 MW; C8B52C0462A37A3A CRC64;
MSLPAHLQQT FSPEEIQFIV ENEPIKIFPR ITTRQKIRGD DRGTGNHTRW QLITTDDKAL
NNMVAMRSTE VVLWIALLLK QQSKCSIVAP QWLTTKELDR KIQYEKTHPD RFSELPWNWL
VLARILFNKA KDDFHDPIHE LRGKIQDLRE IRQIKVLKGL KYLNESHLQL DNLSLLEINE
LRPFITEIMD KLREIHTASL TAGTENDEEE FNI
