PSF3_MOUSE
ID PSF3_MOUSE Reviewed; 216 AA.
AC Q9CY94;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 124.
DE RecName: Full=DNA replication complex GINS protein PSF3;
DE AltName: Full=GINS complex subunit 3;
GN Name=Gins3; Synonyms=Psf3;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Bone marrow, and Heart;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=CD-1, and Czech II; TISSUE=Lung, and Neural stem cell;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: The GINS complex plays an essential role in the initiation of
CC DNA replication, and progression of DNA replication forks. GINS complex
CC seems to bind preferentially to single-stranded DNA.
CC {ECO:0000250|UniProtKB:Q9BRX5}.
CC -!- SUBUNIT: Component of the GINS complex which is a heterotetramer of
CC GINS1, GINS2, GINS3 and GINS4. Forms a stable subcomplex with GINS2.
CC GINS complex interacts with DNA primase in vitro (By similarity).
CC Component of the CMG helicase complex, composed of the MCM2-7 complex,
CC the GINS complex and CDC45 (By similarity).
CC {ECO:0000250|UniProtKB:Q7ZT01, ECO:0000250|UniProtKB:Q9BRX5}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q7ZT01}.
CC Chromosome {ECO:0000250|UniProtKB:Q7ZT01}. Note=Associates with
CC chromatin. {ECO:0000250|UniProtKB:Q7ZT01}.
CC -!- SIMILARITY: Belongs to the GINS3/PSF3 family. {ECO:0000305}.
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DR EMBL; AK019225; BAB31610.1; -; mRNA.
DR EMBL; AK146574; BAE27270.1; -; mRNA.
DR EMBL; AK150602; BAE29694.1; -; mRNA.
DR EMBL; AK151243; BAE30235.1; -; mRNA.
DR EMBL; AK152572; BAE31324.1; -; mRNA.
DR EMBL; AK160606; BAE35909.1; -; mRNA.
DR EMBL; BC039794; AAH39794.1; -; mRNA.
DR EMBL; BC064746; AAH64746.1; -; mRNA.
DR CCDS; CCDS22564.1; -.
DR RefSeq; NP_084474.1; NM_030198.3.
DR AlphaFoldDB; Q9CY94; -.
DR SMR; Q9CY94; -.
DR BioGRID; 219666; 1.
DR ComplexPortal; CPX-4502; GINS complex.
DR STRING; 10090.ENSMUSP00000034094; -.
DR PhosphoSitePlus; Q9CY94; -.
DR EPD; Q9CY94; -.
DR MaxQB; Q9CY94; -.
DR PaxDb; Q9CY94; -.
DR PeptideAtlas; Q9CY94; -.
DR PRIDE; Q9CY94; -.
DR ProteomicsDB; 291659; -.
DR Antibodypedia; 29079; 95 antibodies from 20 providers.
DR DNASU; 78833; -.
DR Ensembl; ENSMUST00000034094; ENSMUSP00000034094; ENSMUSG00000031669.
DR GeneID; 78833; -.
DR KEGG; mmu:78833; -.
DR UCSC; uc009myr.1; mouse.
DR CTD; 64785; -.
DR MGI; MGI:1926083; Gins3.
DR VEuPathDB; HostDB:ENSMUSG00000031669; -.
DR eggNOG; KOG1106; Eukaryota.
DR GeneTree; ENSGT00390000001622; -.
DR HOGENOM; CLU_081646_2_0_1; -.
DR InParanoid; Q9CY94; -.
DR OMA; IYKEGWR; -.
DR OrthoDB; 1291729at2759; -.
DR PhylomeDB; Q9CY94; -.
DR TreeFam; TF314626; -.
DR Reactome; R-MMU-176974; Unwinding of DNA.
DR BioGRID-ORCS; 78833; 28 hits in 74 CRISPR screens.
DR ChiTaRS; Gins3; mouse.
DR PRO; PR:Q9CY94; -.
DR Proteomes; UP000000589; Chromosome 8.
DR RNAct; Q9CY94; protein.
DR Bgee; ENSMUSG00000031669; Expressed in manus and 174 other tissues.
DR ExpressionAtlas; Q9CY94; baseline and differential.
DR Genevisible; Q9CY94; MM.
DR GO; GO:0071162; C:CMG complex; ISS:UniProtKB.
DR GO; GO:0000811; C:GINS complex; ISO:MGI.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0005634; C:nucleus; IC:ComplexPortal.
DR GO; GO:0006268; P:DNA unwinding involved in DNA replication; ISO:MGI.
DR GO; GO:1902975; P:mitotic DNA replication initiation; IBA:GO_Central.
DR GO; GO:1903934; P:positive regulation of DNA primase activity; ISO:MGI.
DR GO; GO:1900264; P:positive regulation of DNA-directed DNA polymerase activity; ISO:MGI.
DR Gene3D; 1.20.58.2050; -; 1.
DR InterPro; IPR021151; GINS_A.
DR InterPro; IPR036224; GINS_bundle-like_dom_sf.
DR InterPro; IPR010492; GINS_Psf3.
DR InterPro; IPR038437; GINS_Psf3_sf.
DR PANTHER; PTHR22768; PTHR22768; 1.
DR Pfam; PF05916; Sld5; 1.
DR SUPFAM; SSF158573; SSF158573; 1.
PE 2: Evidence at transcript level;
KW Chromosome; DNA replication; Nucleus; Reference proteome.
FT CHAIN 1..216
FT /note="DNA replication complex GINS protein PSF3"
FT /id="PRO_0000327616"
FT REGION 1..16
FT /note="Not essential for folding and stability of GINS
FT complex, but may regulate accessibility to the central
FT complex pore"
FT /evidence="ECO:0000250"
SQ SEQUENCE 216 AA; 24577 MW; A131F7176A2498C0 CRC64;
MSEAYFPVES GALGPEENFL SLDDILMSQE KLPVRVETPM PRLGAFFLER GAGSEPDHPL
PQGTKLELPL WLAKGLFDHK RRILSVELPK MYQEGWRTVF SADANVVDLH KMGPHFYGFG
SQLLHFDSPE NADISQSLLK TFIGRFRRIM DSSQNSYNED TSALVARLDE TERGLFQIGQ
RSLNDFQSWE KGQASQITAS SLVQNYKKRK FTNMED
