PSF3_YEAST
ID PSF3_YEAST Reviewed; 194 AA.
AC Q12146; D6W1S3;
DT 15-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT 15-MAR-2005, sequence version 2.
DT 03-AUG-2022, entry version 150.
DE RecName: Full=DNA replication complex GINS protein PSF3;
DE AltName: Full=Partner of Sld five 3;
GN Name=PSF3 {ECO:0000312|SGD:S000005506}; OrderedLocusNames=YOL146W;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1] {ECO:0000312|EMBL:CAA88281.1}
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 96604 / S288c / FY1679;
RX PubMed=8553699; DOI=10.1002/yea.320111308;
RA Casamayor A., Aldea M., Casas C., Herrero E., Gamo F.-J., Lafuente M.J.,
RA Gancedo C., Arino J.;
RT "DNA sequence analysis of a 13 kbp fragment of the left arm of yeast
RT chromosome XV containing seven new open reading frames.";
RL Yeast 11:1281-1288(1995).
RN [2] {ECO:0000305, ECO:0000312|EMBL:CAA99167.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169874;
RA Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J.,
RA Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A.,
RA Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B.,
RA Dang V.-D., de Haan M., Delius H., Durand P., Fairhead C., Feldmann H.,
RA Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E.,
RA Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U.,
RA Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B.,
RA Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A.,
RA Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C.,
RA Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G.,
RA Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B.,
RA Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B.,
RA Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F.,
RA Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E.,
RA Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I.,
RA Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H.,
RA Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV.";
RL Nature 387:98-102(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY.
RX PubMed=14690591; DOI=10.1016/s1097-2765(03)00476-3;
RA Hazbun T.R., Malmstroem L., Anderson S., Graczyk B.J., Fox B., Riffle M.,
RA Sundin B.A., Aranda J.D., McDonald W.H., Chiu C.-H., Snydsman B.E.,
RA Bradley P., Muller E.G.D., Fields S., Baker D., Yates J.R. III, Davis T.N.;
RT "Assigning function to yeast proteins by integration of technologies.";
RL Mol. Cell 12:1353-1365(2003).
RN [6] {ECO:0000305}
RP IDENTIFICATION OF PROBABLE INITIATION SITE.
RX PubMed=12748633; DOI=10.1038/nature01644;
RA Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S.;
RT "Sequencing and comparison of yeast species to identify genes and
RT regulatory elements.";
RL Nature 423:241-254(2003).
RN [7] {ECO:0000305}
RP FUNCTION, IDENTIFICATION IN THE GINS COMPLEX, AND SUBCELLULAR LOCATION.
RX PubMed=12730134; DOI=10.1101/gad.1065903;
RA Takayama Y., Kamimura Y., Okawa M., Muramatsu S., Sugino A., Araki H.;
RT "GINS, a novel multiprotein complex required for chromosomal DNA
RT replication in budding yeast.";
RL Genes Dev. 17:1153-1165(2003).
RN [8] {ECO:0000305}
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
CC -!- FUNCTION: Functions as part of the GINS complex which plays an
CC essential role in the initiation of DNA replication by binding to DNA
CC replication origins and facilitating the assembly of the DNA
CC replication machinery. {ECO:0000250|UniProtKB:P40359,
CC ECO:0000269|PubMed:12730134}.
CC -!- SUBUNIT: Component of the GINS complex which is a heterotetramer of
CC SLD5, PSF1, PSF2 and PSF3. {ECO:0000269|PubMed:12730134}.
CC -!- INTERACTION:
CC Q12146; P40359: PSF2; NbExp=5; IntAct=EBI-30392, EBI-25936;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:12730134}.
CC -!- MISCELLANEOUS: Present with 2205 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the GINS3/PSF3 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAT93239.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=CAA88281.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=CAA99167.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; Z48239; CAA88281.1; ALT_INIT; Genomic_DNA.
DR EMBL; Z74888; CAA99167.1; ALT_INIT; Genomic_DNA.
DR EMBL; AY693220; AAT93239.1; ALT_INIT; Genomic_DNA.
DR EMBL; BK006948; DAA10639.1; -; Genomic_DNA.
DR PIR; S60390; S60390.
DR RefSeq; NP_014495.2; NM_001183400.1.
DR PDB; 3JC5; EM; 4.70 A; C=1-194.
DR PDB; 3JC6; EM; 3.70 A; C=1-194.
DR PDB; 3JC7; EM; 4.80 A; C=1-194.
DR PDB; 5U8S; EM; 6.10 A; C=1-194.
DR PDB; 5U8T; EM; 4.90 A; C=1-194.
DR PDB; 6HV9; EM; 4.98 A; E=1-194.
DR PDB; 6PTJ; EM; 3.80 A; C=1-194.
DR PDB; 6PTN; EM; 5.80 A; C/c=1-194.
DR PDB; 6PTO; EM; 7.00 A; C/c/p=1-194.
DR PDB; 6SKL; EM; 3.70 A; C=1-194.
DR PDB; 6U0M; EM; 3.90 A; C=3-193.
DR PDBsum; 3JC5; -.
DR PDBsum; 3JC6; -.
DR PDBsum; 3JC7; -.
DR PDBsum; 5U8S; -.
DR PDBsum; 5U8T; -.
DR PDBsum; 6HV9; -.
DR PDBsum; 6PTJ; -.
DR PDBsum; 6PTN; -.
DR PDBsum; 6PTO; -.
DR PDBsum; 6SKL; -.
DR PDBsum; 6U0M; -.
DR AlphaFoldDB; Q12146; -.
DR SMR; Q12146; -.
DR BioGRID; 34271; 58.
DR ComplexPortal; CPX-1641; GINS complex.
DR DIP; DIP-4461N; -.
DR IntAct; Q12146; 24.
DR MINT; Q12146; -.
DR STRING; 4932.YOL146W; -.
DR MaxQB; Q12146; -.
DR PaxDb; Q12146; -.
DR PRIDE; Q12146; -.
DR EnsemblFungi; YOL146W_mRNA; YOL146W; YOL146W.
DR GeneID; 854019; -.
DR KEGG; sce:YOL146W; -.
DR SGD; S000005506; PSF3.
DR VEuPathDB; FungiDB:YOL146W; -.
DR eggNOG; KOG1106; Eukaryota.
DR GeneTree; ENSGT00390000001622; -.
DR HOGENOM; CLU_081646_0_1_1; -.
DR InParanoid; Q12146; -.
DR OMA; IYKEGWR; -.
DR BioCyc; YEAST:G3O-33536-MON; -.
DR Reactome; R-SCE-176974; Unwinding of DNA.
DR PRO; PR:Q12146; -.
DR Proteomes; UP000002311; Chromosome XV.
DR RNAct; Q12146; protein.
DR GO; GO:0071162; C:CMG complex; IDA:SGD.
DR GO; GO:0031261; C:DNA replication preinitiation complex; IPI:SGD.
DR GO; GO:0000811; C:GINS complex; IPI:SGD.
DR GO; GO:0043596; C:nuclear replication fork; IDA:ComplexPortal.
DR GO; GO:0005634; C:nucleus; IC:ComplexPortal.
DR GO; GO:0031298; C:replication fork protection complex; IDA:SGD.
DR GO; GO:0006268; P:DNA unwinding involved in DNA replication; IDA:ComplexPortal.
DR GO; GO:0006261; P:DNA-templated DNA replication; IGI:SGD.
DR GO; GO:0000727; P:double-strand break repair via break-induced replication; IMP:SGD.
DR GO; GO:1902975; P:mitotic DNA replication initiation; IBA:GO_Central.
DR GO; GO:1903934; P:positive regulation of DNA primase activity; IC:ComplexPortal.
DR GO; GO:1900264; P:positive regulation of DNA-directed DNA polymerase activity; IC:ComplexPortal.
DR Gene3D; 1.20.58.2050; -; 1.
DR InterPro; IPR021151; GINS_A.
DR InterPro; IPR036224; GINS_bundle-like_dom_sf.
DR InterPro; IPR010492; GINS_Psf3.
DR InterPro; IPR038437; GINS_Psf3_sf.
DR PANTHER; PTHR22768; PTHR22768; 1.
DR Pfam; PF05916; Sld5; 1.
DR SUPFAM; SSF158573; SSF158573; 1.
PE 1: Evidence at protein level;
KW 3D-structure; DNA replication; Nucleus; Reference proteome.
FT CHAIN 1..194
FT /note="DNA replication complex GINS protein PSF3"
FT /id="PRO_0000132325"
SQ SEQUENCE 194 AA; 21952 MW; 3E8B60DFCE16391D CRC64;
MGYYDIDDVL ADGTEFPCKF QYDIPGLGYL ENNPGRPITK NTKLSLPLWL ARILAIVGGD
EALVDEEPVP FVELLPPDMF STKVMNAIKT DPVALDLHSI NSHFFSLAIK WIMLFSEKEL
ANVVSELLLQ RAQELNHHAS SLSIDLNADS TGKNSANTNI ATSTFLLKLE EMEKEIYKKS
HESYKDTKRW MFKK
