PSI1_ARATH
ID PSI1_ARATH Reviewed; 657 AA.
AC Q9XID5; Q6NQ48;
DT 22-APR-2020, integrated into UniProtKB/Swiss-Prot.
DT 22-APR-2020, sequence version 2.
DT 25-MAY-2022, entry version 64.
DE RecName: Full=Protein PSK SIMULATOR 1 {ECO:0000303|PubMed:25062973};
DE Short=AtPSI1 {ECO:0000303|PubMed:25062973};
GN Name=PSI1 {ECO:0000303|PubMed:25062973};
GN OrderedLocusNames=At1g34320 {ECO:0000312|Araport:AT1G34320};
GN ORFNames=F23M19.3 {ECO:0000312|EMBL:AAD39604.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Cheuk R.F., Chen H., Kim C.J., Shinn P., Carninci P., Hayashizaki Y.,
RA Ishida J., Kamiya A., Kawai J., Narusaka M., Sakurai T., Satou M., Seki M.,
RA Shinozaki K., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP FUNCTION, MUTAGENESIS OF GLY-2, DISRUPTION PHENOTYPE, AND SUBCELLULAR
RP LOCATION.
RC STRAIN=cv. Columbia;
RX PubMed=25062973; DOI=10.1007/s11103-014-0229-2;
RA Stuehrwohldt N., Hartmann J., Dahlke R.I., Oecking C., Sauter M.;
RT "The PSI family of nuclear proteins is required for growth in
RT arabidopsis.";
RL Plant Mol. Biol. 86:289-302(2014).
CC -!- FUNCTION: Promotes seedling growth probably via the regulation of
CC phytosulfokine (PSK) signaling; PSK are peptide phytohormones acting as
CC growth factors (PubMed:25062973). Together with PSI2 and PSI3, required
CC during vegetative growth and reproduction (PubMed:25062973). May also
CC have a function in carbohydrate metabolism (PubMed:25062973).
CC {ECO:0000269|PubMed:25062973}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:25062973}.
CC -!- DISRUPTION PHENOTYPE: Short roots and hypocotyls due to both a reduced
CC cell number and reduced cell elongation (PubMed:25062973). Plants
CC missing both PSI1 and PSI3 are dwarf and contain reduced starch levels;
CC these phenotypes are partially rescued by sucrose (PubMed:25062973).
CC {ECO:0000269|PubMed:25062973}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAD39604.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AC007454; AAD39604.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE31697.1; -; Genomic_DNA.
DR EMBL; CP002684; ANM60400.1; -; Genomic_DNA.
DR EMBL; BT010615; AAQ89637.1; -; mRNA.
DR EMBL; AK176401; BAD44164.1; -; mRNA.
DR EMBL; AK176473; BAD44236.1; -; mRNA.
DR PIR; D86467; D86467.
DR RefSeq; NP_001322690.1; NM_001333096.1.
DR RefSeq; NP_174692.1; NM_103154.4.
DR AlphaFoldDB; Q9XID5; -.
DR IntAct; Q9XID5; 3.
DR STRING; 3702.AT1G34320.1; -.
DR iPTMnet; Q6NQ48; -.
DR ProteomicsDB; 181282; -.
DR EnsemblPlants; AT1G34320.1; AT1G34320.1; AT1G34320.
DR EnsemblPlants; AT1G34320.2; AT1G34320.2; AT1G34320.
DR GeneID; 840333; -.
DR Gramene; AT1G34320.1; AT1G34320.1; AT1G34320.
DR Gramene; AT1G34320.2; AT1G34320.2; AT1G34320.
DR KEGG; ath:AT1G34320; -.
DR Araport; AT1G34320; -.
DR TAIR; locus:2026135; AT1G34320.
DR eggNOG; ENOG502QQVZ; Eukaryota.
DR HOGENOM; CLU_022639_0_0_1; -.
DR OMA; VNDGIPH; -.
DR OrthoDB; 372706at2759; -.
DR PRO; PR:Q9XID5; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9XID5; baseline and differential.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0045927; P:positive regulation of growth; IMP:UniProtKB.
DR GO; GO:0006109; P:regulation of carbohydrate metabolic process; IMP:UniProtKB.
DR GO; GO:0043434; P:response to peptide hormone; IMP:UniProtKB.
DR InterPro; IPR021864; DUF3475.
DR InterPro; IPR007700; DUF668.
DR InterPro; IPR045021; PSI1/2/3.
DR PANTHER; PTHR31730; PTHR31730; 1.
DR Pfam; PF11961; DUF3475; 1.
DR Pfam; PF05003; DUF668; 1.
PE 1: Evidence at protein level;
KW Developmental protein; Lipoprotein; Myristate; Nucleus; Reference proteome.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000255"
FT CHAIN 2..657
FT /note="Protein PSK SIMULATOR 1"
FT /id="PRO_0000449314"
FT REGION 1..80
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 534..559
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..42
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 60..77
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 537..559
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT LIPID 2
FT /note="N-myristoyl glycine"
FT /evidence="ECO:0000255"
FT MUTAGEN 2
FT /note="G->A: Normal phenotype. Able to complement the
FT growth-retarded phenotype of plants lacking PSI1."
FT /evidence="ECO:0000269|PubMed:25062973"
SQ SEQUENCE 657 AA; 72841 MW; 1683E5C4AAFB9F87 CRC64;
MGGLCSRSSS VNNAPGGTFA HVNGHHLNNN ASDLNSHSGE SGLKDDPSPV TENVDDNKHT
SESFSFPIVS SGSHPQNIED GIPRLSRVLS QKSRSTKSRQ AAVAKVSEVS SLLGRAGTMG
LGKAVDVLDT LGSSMTNLNL SGGFSSATTV KGNKISILSF EVANTIVKGA NLMHSLSKDS
ITHLKEVVLP SEGVQNLISK DMDELLRIAA ADKREELRIF SGEVVRFGNR CKDPQYHNLD
RFFDRLGSEF TPQKHLKQEA ETIMHQMMSF VHFTADLYHE LHALDRFEQD YQRKIQEEEN
PSTAQRGVGD TLAILRTELK SQKKHVRNLK KKSLWSRILE EVMEKLVDVV HFLHLEIHEA
FGGADPDKPA NDPPINHKKL GSAGLALHYA NIITQIDTLV SRSSTMPAST RDALYQGLPP
SIKSALRSRI QSFQVKEELT VPQIKAEMEK TLQWLVPVAT NTTKAHHGFG WVGEWASSGS
EANQRPAGQT ILRIDTLHHA DKEKTEAYIL DLVVWLHHLV TQVRATTGYG LRSPVKSPIR
SPNQKTIQLS SGSHNPSMGL PLLTTEDQEM LRDVSKRRKT PGISKSQEFE TVAKARLCKH
HRLSKSSSHS PMMGEMMKNK KDTFSTRRPS SVPIIDFDID RMKALDVIDR VDTIRSL
