PSI2_ARATH
ID PSI2_ARATH Reviewed; 615 AA.
AC Q9SA91; Q8L5Y3;
DT 22-APR-2020, integrated into UniProtKB/Swiss-Prot.
DT 22-APR-2020, sequence version 2.
DT 25-MAY-2022, entry version 68.
DE RecName: Full=Protein PSK SIMULATOR 2 {ECO:0000303|PubMed:25062973};
DE Short=AtPSI2 {ECO:0000303|PubMed:25062973};
GN Name=PSI2 {ECO:0000303|PubMed:25062973};
GN OrderedLocusNames=At1g30755 {ECO:0000312|Araport:AT1G30755};
GN ORFNames=T5I8.21 {ECO:0000312|EMBL:AAD25764.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP FUNCTION, DISRUPTION PHENOTYPE, AND SUBCELLULAR LOCATION.
RC STRAIN=cv. Columbia;
RX PubMed=25062973; DOI=10.1007/s11103-014-0229-2;
RA Stuehrwohldt N., Hartmann J., Dahlke R.I., Oecking C., Sauter M.;
RT "The PSI family of nuclear proteins is required for growth in
RT arabidopsis.";
RL Plant Mol. Biol. 86:289-302(2014).
CC -!- FUNCTION: Promotes seedling growth probably via the regulation of
CC phytosulfokine (PSK) signaling; PSK are peptide phytohormones acting as
CC growth factors (PubMed:25062973). Involved in PSK-induced root growth
CC (PubMed:25062973). Together with PSI1 and PSI3, required during
CC vegetative growth and reproduction (PubMed:25062973).
CC {ECO:0000269|PubMed:25062973}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:25062973}.
CC -!- DISRUPTION PHENOTYPE: Reduced fertility, premature senescence and
CC severe dwarfism in psi2-1 psi3-1 plants due to reduced cell growth and
CC proliferation as well as premature leaf growth arrest.
CC {ECO:0000269|PubMed:25062973}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAD25764.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC007060; AAD25764.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE31268.1; -; Genomic_DNA.
DR EMBL; CP002684; ANM58089.1; -; Genomic_DNA.
DR EMBL; CP002684; ANM58091.1; -; Genomic_DNA.
DR EMBL; AY099867; AAM20718.1; -; mRNA.
DR PIR; C86433; C86433.
DR RefSeq; NP_001320549.1; NM_001332926.1.
DR RefSeq; NP_001320551.1; NM_001332925.1.
DR RefSeq; NP_564362.1; NM_102812.3.
DR AlphaFoldDB; Q9SA91; -.
DR SMR; Q9SA91; -.
DR STRING; 3702.AT1G30755.1; -.
DR ProteomicsDB; 175574; -.
DR EnsemblPlants; AT1G30755.1; AT1G30755.1; AT1G30755.
DR EnsemblPlants; AT1G30755.5; AT1G30755.5; AT1G30755.
DR EnsemblPlants; AT1G30755.6; AT1G30755.6; AT1G30755.
DR GeneID; 839956; -.
DR Gramene; AT1G30755.1; AT1G30755.1; AT1G30755.
DR Gramene; AT1G30755.5; AT1G30755.5; AT1G30755.
DR Gramene; AT1G30755.6; AT1G30755.6; AT1G30755.
DR KEGG; ath:AT1G30755; -.
DR Araport; AT1G30755; -.
DR TAIR; locus:505006158; AT1G30755.
DR eggNOG; ENOG502QUNR; Eukaryota.
DR HOGENOM; CLU_022639_0_0_1; -.
DR OMA; WANSRIE; -.
DR OrthoDB; 372706at2759; -.
DR PRO; PR:Q9SA91; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9SA91; baseline and differential.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0003746; F:translation elongation factor activity; IEA:UniProtKB-KW.
DR GO; GO:0045927; P:positive regulation of growth; IMP:UniProtKB.
DR GO; GO:0043434; P:response to peptide hormone; IMP:UniProtKB.
DR InterPro; IPR021864; DUF3475.
DR InterPro; IPR007700; DUF668.
DR InterPro; IPR045021; PSI1/2/3.
DR PANTHER; PTHR31730; PTHR31730; 1.
DR Pfam; PF11961; DUF3475; 1.
DR Pfam; PF05003; DUF668; 1.
PE 2: Evidence at transcript level;
KW Developmental protein; Elongation factor; Lipoprotein; Myristate; Nucleus;
KW Protein biosynthesis; Reference proteome.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000255"
FT CHAIN 2..615
FT /note="Protein PSK SIMULATOR 2"
FT /id="PRO_0000449315"
FT REGION 16..59
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 506..529
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 16..41
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 514..529
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT LIPID 2
FT /note="N-myristoyl glycine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 615 AA; 69395 MW; 560E85D4A01E265E CRC64;
MGGVCSCVFK DDDKKKKLRS NDDDKSRGFS GKLKSMRRSK TSDSYYSDNY GGSRRKSSKP
DEVVFNFSGE LGPMPPLRND STKFMQRNSF MGRAGVMGLE KAVEVLDTLG SSMTRMNPSN
AYLSGVTSSR GGKVTILAFE VANTIAKGAA LLQSLSEENL KFMKKDMLHS EEVKKLVSTD
TTELQILAAS DKREELDLFS GEVIRFGNMC KDLQWHNLDR YFMKLDTENS QHKLLKDDAE
ARMQELVTLA RITSELYHEL QALDRFEQDY RRKLAEVESL NLPRRGEGIV ILQNELKQQK
KLVKSLQKKS LWSQNLAEII EKLVDVVSYI RQTIVEVFGN NGLRDNEGEQ GRERLGEAGL
SLHYANLIQQ IDNIASRPSS LPSNVRDTLY NALPATVKTA LRPRLQTLDQ EEELSVPEIK
AEMEKSLQWL VPFAENTTKA HQGFGWVGEW ANSRIEFGKG KGKGENNGNP TRLQTLHHAD
KPIVDSYVLE LVVWLHRLMK SSKKRAHGVK LQETNHVSPP NNRTISNTQL SLSPDFTYKN
QLSLEDRLLL DRVQSIRFGP NLSKSQELVG LKKNKKGFKI WALSRSTGNS PKVDLSDKNS
SSDLDVLDGL DFAFQ
