PSI3_ARATH
ID PSI3_ARATH Reviewed; 649 AA.
AC P0DO24; A0A178UKE1; Q9C5B1;
DT 22-APR-2020, integrated into UniProtKB/Swiss-Prot.
DT 22-APR-2020, sequence version 1.
DT 03-AUG-2022, entry version 10.
DE RecName: Full=Protein PSK SIMULATOR 3 {ECO:0000303|PubMed:25062973};
DE Short=AtPSI3 {ECO:0000303|PubMed:25062973};
GN Name=PSI3 {ECO:0000303|PubMed:25062973};
GN OrderedLocusNames=At5g08660 {ECO:0000312|Araport:AT5G08660};
GN ORFNames=T2K12 {ECO:0000312|EMBL:CAC35871.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130714; DOI=10.1038/35048507;
RA Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA Bevan M., Fransz P.F.;
RT "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL Nature 408:823-826(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19376835; DOI=10.1104/pp.109.138677;
RA Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A.,
RA Grossmann J., Gruissem W., Baginsky S.;
RT "Large-scale Arabidopsis phosphoproteome profiling reveals novel
RT chloroplast kinase substrates and phosphorylation networks.";
RL Plant Physiol. 150:889-903(2009).
RN [4]
RP FUNCTION, DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION, AND INDUCTION BY
RP SUCROSE.
RC STRAIN=cv. Columbia;
RX PubMed=25062973; DOI=10.1007/s11103-014-0229-2;
RA Stuehrwohldt N., Hartmann J., Dahlke R.I., Oecking C., Sauter M.;
RT "The PSI family of nuclear proteins is required for growth in
RT arabidopsis.";
RL Plant Mol. Biol. 86:289-302(2014).
CC -!- FUNCTION: Promotes plant growth, especially at the vegetative stage,
CC probably via the regulation of phytosulfokine (PSK) signaling; PSK are
CC peptide phytohormones acting as growth factors (PubMed:25062973).
CC Together with PSI2 and PSI3, required during vegetative growth and
CC reproduction (PubMed:25062973). May also have a function in
CC carbohydrate metabolism (PubMed:25062973).
CC {ECO:0000269|PubMed:25062973}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:25062973}.
CC -!- INDUCTION: By sucrose. {ECO:0000269|PubMed:25062973}.
CC -!- DISRUPTION PHENOTYPE: Reduced response to phytosulfokine (PSK) in roots
CC (PubMed:25062973). Small rosettes and dwarf plants as well as early
CC senescence of older leaves (PubMed:25062973). Reduced fertility,
CC premature senescence and severe dwarfism in psi2-1 psi3-1 plants due to
CC reduced cell growth and proliferation as well as premature leaf growth
CC arrest (PubMed:25062973). Plants missing both PSI1 and PSI3 are dwarf
CC and contain reduced starch levels; these phenotypes are partially
CC rescued by sucrose (PubMed:25062973). {ECO:0000269|PubMed:25062973}.
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DR EMBL; AL590346; CAC35871.1; -; Genomic_DNA.
DR EMBL; CP002688; AED91335.1; -; Genomic_DNA.
DR EMBL; CP002688; ANM69947.1; -; Genomic_DNA.
DR EMBL; CP002688; ANM69948.1; -; Genomic_DNA.
DR RefSeq; NP_001331591.1; NM_001343028.1.
DR RefSeq; NP_001331592.1; NM_001343029.1.
DR RefSeq; NP_680154.2; NM_147849.4.
DR AlphaFoldDB; P0DO24; -.
DR SMR; P0DO24; -.
DR STRING; 3702.AT5G08660.1; -.
DR iPTMnet; Q9C5B1; -.
DR ProteomicsDB; 189278; -.
DR EnsemblPlants; AT5G08660.1; AT5G08660.1; AT5G08660.
DR EnsemblPlants; AT5G08660.3; AT5G08660.3; AT5G08660.
DR EnsemblPlants; AT5G08660.4; AT5G08660.4; AT5G08660.
DR GeneID; 830767; -.
DR Gramene; AT5G08660.1; AT5G08660.1; AT5G08660.
DR Gramene; AT5G08660.3; AT5G08660.3; AT5G08660.
DR Gramene; AT5G08660.4; AT5G08660.4; AT5G08660.
DR KEGG; ath:AT5G08660; -.
DR Araport; AT5G08660; -.
DR TAIR; locus:504956337; AT5G08660.
DR eggNOG; ENOG502QQVZ; Eukaryota.
DR HOGENOM; CLU_022639_0_0_1; -.
DR OrthoDB; 372706at2759; -.
DR PRO; PR:P0DO24; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; P0DO24; baseline and differential.
DR GO; GO:0005739; C:mitochondrion; HDA:TAIR.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0005886; C:plasma membrane; HDA:TAIR.
DR GO; GO:0045927; P:positive regulation of growth; IMP:UniProtKB.
DR GO; GO:0006109; P:regulation of carbohydrate metabolic process; IMP:UniProtKB.
DR GO; GO:0043434; P:response to peptide hormone; IMP:UniProtKB.
DR GO; GO:0009744; P:response to sucrose; IEP:UniProtKB.
DR InterPro; IPR021864; DUF3475.
DR InterPro; IPR007700; DUF668.
DR InterPro; IPR045021; PSI1/2/3.
DR PANTHER; PTHR31730; PTHR31730; 1.
DR Pfam; PF11961; DUF3475; 1.
DR Pfam; PF05003; DUF668; 1.
PE 1: Evidence at protein level;
KW Developmental protein; Lipoprotein; Myristate; Nucleus; Reference proteome.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000255"
FT CHAIN 2..649
FT /note="Protein PSK SIMULATOR 3"
FT /id="PRO_0000449316"
FT REGION 18..43
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT LIPID 2
FT /note="N-myristoyl glycine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 649 AA; 72498 MW; F8E2EA8C7442DF85 CRC64;
MGSFCSKSLG INFGSEYSGS SVADDGREPD FGHSQPNGQT SLIVPGMRQL MVKDVKEQNQ
LKDVFSFRER EAEDNFYDGI PTYTMAPSQK IRSAKSTQTA VSKVTEASKL LGKAGLGRAK
DVLDTLGSSM TDLSSGGFTS GVATKGNELG ILAFEVANTI VKSSNLIESL SKRNIEHLKG
TILYSEGVQN LVSNDFDELL RLVAADKRQE LQVFSGEVVR FGNRSKDFQW HNLQRYFDRI
SKELTPQRQL KEDAVLVVDQ LMVLVQYTAE LYQELQVLYR LEKDYEQKRR EEENSANSSK
GDGLAILKTE LKAQRKVVKS LKKKSLWSRG FEEVMEKLVD IVHFLLLEIH NIFGGADDQP
SKKGAAEYDK RLGPAGLALH YANIIVQIDT LVARASSITS NARDSLYQSL PPGIKLALRS
KIKSFNVDKE LSVTQIKDEM ERTLHWLVPV AGNTTKAHHG FGWVGEWANT GTDFTSKPSG
GDILRIETLY HASKEKTEIY ILGQIIWLQH LVTKAKSDAR GGPRLSSIKS PLDTTNQQLI
SEPLSVPIVT DEEQKMLQEA SKRKRTPCVS KSQDFDSEYS RARKCDPLSK SSEYFRGVRR
SKSAAVKRYS SGFPLLDFAI DKEKVLDVID RVDVPRDYKA LLKEGSLSF
