PSIA_DICDI
ID PSIA_DICDI Reviewed; 557 AA.
AC Q968Z6; Q54DV4;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 25-MAY-2022, entry version 80.
DE RecName: Full=Protein psiA;
DE AltName: Full=Prespore-cell-inducing factor A;
DE Flags: Precursor;
GN Name=psiA; ORFNames=DDB_G0291982;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 20-26, FUNCTION,
RP DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE.
RX PubMed=15367206; DOI=10.1111/j.1440-169x.2004.00749.x;
RA Kawata T., Nakagawa M., Shimada N., Fujii S., Oohata A.A.;
RT "A gene encoding, prespore-cell-inducing factor in Dictyostelium
RT discoideum.";
RL Dev. Growth Differ. 46:383-392(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [3]
RP PROTEIN SEQUENCE OF 54-67; 90-95 AND 119-146, FUNCTION, GLYCOSYLATION,
RP SUBUNIT, AND SUBCELLULAR LOCATION.
RX PubMed=10493938; DOI=10.1042/bj3430265;
RA Nakagawa M., Oohata A.A., Tojo H., Fujii S.;
RT "A prespore-cell-inducing factor in Dictyostelium discoideum: its
RT purification and characterization.";
RL Biochem. J. 343:265-271(1999).
CC -!- FUNCTION: Growth factor that can induce isolated amoebae to
CC differentiate into prespore cells. {ECO:0000269|PubMed:10493938,
CC ECO:0000269|PubMed:15367206}.
CC -!- SUBUNIT: Homodimer. {ECO:0000305|PubMed:10493938}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:10493938}.
CC -!- DEVELOPMENTAL STAGE: Expressed maximally at the loose mound stage.
CC {ECO:0000269|PubMed:15367206}.
CC -!- PTM: N-glycosylated. {ECO:0000269|PubMed:10493938}.
CC -!- DISRUPTION PHENOTYPE: Cells appear to develop normally; however,
CC conditioned medium from these mutants show reduced prespore-cell-
CC inducing activity. Conditioned medium from mutant show no cell division
CC inducing activity. {ECO:0000269|PubMed:15367206}.
CC -!- SIMILARITY: Belongs to the prespore-cell-inducing factor family.
CC {ECO:0000305}.
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DR EMBL; AB053203; BAB47241.1; -; mRNA.
DR EMBL; AAFI02000187; EAL61347.1; -; Genomic_DNA.
DR RefSeq; XP_629768.1; XM_629766.1.
DR AlphaFoldDB; Q968Z6; -.
DR STRING; 44689.DDB0201657; -.
DR PaxDb; Q968Z6; -.
DR EnsemblProtists; EAL61347; EAL61347; DDB_G0291982.
DR GeneID; 8628444; -.
DR KEGG; ddi:DDB_G0291982; -.
DR dictyBase; DDB_G0291982; psiA.
DR HOGENOM; CLU_024170_0_0_1; -.
DR PhylomeDB; Q968Z6; -.
DR PRO; PR:Q968Z6; -.
DR Proteomes; UP000002195; Chromosome 6.
DR GO; GO:0005576; C:extracellular region; IDA:dictyBase.
DR GO; GO:0008083; F:growth factor activity; TAS:dictyBase.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0031286; P:negative regulation of sorocarp stalk cell differentiation; IMP:dictyBase.
DR GO; GO:1901263; P:positive regulation of sorocarp spore cell differentiation; IDA:dictyBase.
DR InterPro; IPR011874; Fibro_Slime.
DR InterPro; IPR037524; PA14/GLEYA.
DR InterPro; IPR011658; PA14_dom.
DR Pfam; PF07691; PA14; 1.
DR SMART; SM00758; PA14; 1.
DR TIGRFAMs; TIGR02148; Fibro_Slime; 1.
DR PROSITE; PS51820; PA14; 1.
PE 1: Evidence at protein level;
KW Developmental protein; Differentiation; Direct protein sequencing;
KW Glycoprotein; Growth factor; Reference proteome; Secreted; Signal.
FT SIGNAL 1..19
FT /evidence="ECO:0000269|PubMed:15367206"
FT CHAIN 20..557
FT /note="Protein psiA"
FT /id="PRO_0000327543"
FT DOMAIN 116..261
FT /note="PA14"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01164"
FT REGION 355..451
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 355..414
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 72
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 99
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 155
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 176
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 320
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 329
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 557 AA; 62300 MW; 0D42A60F6185C343 CRC64;
MKLLIYLFIL TFYKTIVLTQ TPSTHLDVDI TIYDQLPLYN NNFEPETGSL TKNLVQTTLG
SDGLPVLNSY SNLTYSNKNG RMYSPELFKY FFAPNQISNT TFNCGRNFPI KKQLKLLRTA
SGNYIYDNDF FFPIDYEGFD TDPANRIYKD DESTNKTYHN YHFCFQFDNR FLFKGNETFK
FTGDDDVWVF INKQLVVDLG GTHPAASSSV DLSTLGLTVG KVYPFNFFYC ERHTSRSTIR
IETSLELYCD KYDYCGVCNG DGSTCCNPIT DCNDDDLCTN DVCPPSDTVI DPSLPISFYC
QHHPTPDPLV SDKCFESVCN STTGSWFLNA TICVEKEGLI NTGCDGNTGC IYEPPTQTPT
ETPTQTPTET PSQTPTETPS QTPTETPSQT PTCTQHPTPT PTCTEHIKTP KPTPTPTCTK
HPKPTPTCTE HTKTPKPTKT LKPTPTPKPT QTPKVPQCGK CEKLSSCKTY CEKKDCDDCQ
RELDGYKFDR CKTYSCDPIK GCIKTDKCKQ GNDPCLKPVC NQYTGFCSQE RVVSDKCKCD
DKGKKGFGLG IGIGINA
