ID   PSIB1_ECOLI             Reviewed;         144 AA.
AC   P10031;
DT   01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1989, sequence version 1.
DT   25-MAY-2022, entry version 89.
DE   RecName: Full=Protein PsiB;
GN   Name=psiB; OrderedLocusNames=ECOK12F063;
OS   Escherichia coli (strain K12).
OG   Plasmid F.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=3205720; DOI=10.1093/nar/16.22.10669;
RA   Dutreix M., Baeckman A., Celerier J., Bagdasarian M.M., Sommer S.,
RA   Bailone A., Devoret R., Bagdasarian M.;
RT   "Identification of psiB genes of plasmids F and R6-5. Molecular basis for
RT   psiB enhanced expression in plasmid R6-5.";
RL   Nucleic Acids Res. 16:10669-10679(1988).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=10366527; DOI=10.1006/plas.1999.1390;
RA   Manwaring N.P., Skurray R.A., Firth N.;
RT   "Nucleotide sequence of the F plasmid leading region.";
RL   Plasmid 41:219-225(1999).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / CR63;
RA   Shimizu H., Saitoh Y., Suda Y., Uehara K., Sampei G., Mizobuchi K.;
RT   "Complete nucleotide sequence of the F plasmid: its implications for
RT   organization and diversification of plasmid genomes.";
RL   Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: In contrast to PsiB protein of plasmid R6-5, this protein is
CC       unable to prevent SOS induction for potentially two reasons: less
CC       activity and/or insufficient expression.
CC   -!- SIMILARITY: To PsiB protein of plasmid R6-5. {ECO:0000305}.
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DR   EMBL; X12462; CAA31003.1; -; Genomic_DNA.
DR   EMBL; AF106329; AAA99213.2; -; Genomic_DNA.
DR   EMBL; AP001918; BAA97933.1; -; Genomic_DNA.
DR   PIR; S01898; S01898.
DR   RefSeq; NP_061442.1; NC_002483.1.
DR   RefSeq; WP_010892539.1; NC_002483.1.
DR   PDB; 3NCT; X-ray; 2.20 A; A/B/C/D=1-144.
DR   PDBsum; 3NCT; -.
DR   AlphaFoldDB; P10031; -.
DR   SMR; P10031; -.
DR   PRIDE; P10031; -.
DR   EvolutionaryTrace; P10031; -.
DR   PRO; PR:P10031; -.
DR   Gene3D; 3.40.50.11880; -; 1.
DR   InterPro; IPR009385; Plasmid_inh_PsiB.
DR   InterPro; IPR038131; PsiB-like_sf.
DR   Pfam; PF06290; PsiB; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Plasmid.
FT   CHAIN           1..144
FT                   /note="Protein PsiB"
FT                   /id="PRO_0000068428"
FT   HELIX           8..12
FT                   /evidence="ECO:0007829|PDB:3NCT"
FT   HELIX           15..24
FT                   /evidence="ECO:0007829|PDB:3NCT"
FT   HELIX           26..38
FT                   /evidence="ECO:0007829|PDB:3NCT"
FT   STRAND          46..50
FT                   /evidence="ECO:0007829|PDB:3NCT"
FT   STRAND          52..54
FT                   /evidence="ECO:0007829|PDB:3NCT"
FT   STRAND          60..68
FT                   /evidence="ECO:0007829|PDB:3NCT"
FT   STRAND          72..78
FT                   /evidence="ECO:0007829|PDB:3NCT"
FT   STRAND          80..83
FT                   /evidence="ECO:0007829|PDB:3NCT"
FT   STRAND          85..93
FT                   /evidence="ECO:0007829|PDB:3NCT"
FT   HELIX           94..96
FT                   /evidence="ECO:0007829|PDB:3NCT"
FT   STRAND          98..107
FT                   /evidence="ECO:0007829|PDB:3NCT"
FT   HELIX           110..125
FT                   /evidence="ECO:0007829|PDB:3NCT"
FT   HELIX           130..141
FT                   /evidence="ECO:0007829|PDB:3NCT"
SQ   SEQUENCE   144 AA;  15853 MW;  F0A321C4A9312195 CRC64;
     MKTELTLNVL QTMNAQEYED IRAAGSDERR ELTHAVMREL DAPDNWTMNG EYGSEFGGFF
     PVQVRFTPAH ERFHLALCSP GDVSQVWVLV LVNAGGEPFA VVQVQRRFAS EAVSHSLALA
     ASLDTQGYSV NDIIHISMAE GGQV