ID   PSID_PSICU              Reviewed;         439 AA.
AC   P0DPA6;
DT   25-OCT-2017, integrated into UniProtKB/Swiss-Prot.
DT   25-OCT-2017, sequence version 1.
DT   25-MAY-2022, entry version 12.
DE   RecName: Full=L-tryptophan decarboxylase {ECO:0000303|PubMed:28763571};
DE            EC=4.1.1.105 {ECO:0000269|PubMed:28763571};
DE   AltName: Full=Psilocybin biosynthesis decarboxylase {ECO:0000303|PubMed:28763571};
GN   Name=psiD {ECO:0000303|PubMed:28763571};
OS   Psilocybe cubensis (Psychedelic mushroom) (Stropharia cubensis).
OC   Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes;
OC   Agaricomycetidae; Agaricales; Strophariaceae; Psilocybe.
OX   NCBI_TaxID=181762;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], IDENTIFICATION, FUNCTION, CATALYTIC ACTIVITY,
RP   AND PATHWAY.
RX   PubMed=28763571; DOI=10.1002/anie.201705489;
RA   Fricke J., Blei F., Hoffmeister D.;
RT   "Enzymatic synthesis of psilocybin.";
RL   Angew. Chem. Int. Ed. 56:12352-12355(2017).
CC   -!- FUNCTION: L-tryptophan decarboxylase; part of the gene cluster that
CC       mediates the biosynthesis of psilocybin, a psychotropic tryptamine-
CC       derived natural product (PubMed:28763571). The first step in the
CC       pathway is the decarboxylation of L-tryptophan to tryptamine by the
CC       decarboxylase psiD (PubMed:28763571). 4-hydroxy-L-tryptophan is
CC       accepted as substrate by psiD as well (PubMed:28763571). The cytochrome
CC       P450 monooxygenase psiH then converts tryptamine to 4-hydroxytryptamine
CC       (PubMed:28763571). The kinase psiK catalyzes the 4-O-phosphorylation
CC       step by converting 4-hydroxytryptamine into norbaeocystin
CC       (PubMed:28763571). The methyltransferase psiM then catalyzes iterative
CC       methyl transfer to the amino group of norbaeocystin to yield psilocybin
CC       via a monomethylated intermediate, baeocystin (PubMed:28763571). PsiK
CC       kinase can also turn psilocin into psilocybin (PubMed:28763571). This
CC       activity may represent a protective mechanism to rephosphorylate the
CC       unstable psilocin to the stable psilocybin in case of intracellular
CC       ester cleavage (PubMed:28763571). {ECO:0000269|PubMed:28763571}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H(+) + L-tryptophan = CO2 + tryptamine; Xref=Rhea:RHEA:30339,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57887,
CC         ChEBI:CHEBI:57912; EC=4.1.1.105;
CC         Evidence={ECO:0000269|PubMed:28763571};
CC   -!- PATHWAY: Secondary metabolite biosynthesis.
CC       {ECO:0000269|PubMed:28763571}.
CC   -!- SIMILARITY: Belongs to the phosphatidylserine decarboxylase family.
CC       {ECO:0000305}.
CC   -!- WEB RESOURCE: Name=Protein Spotlight; Note=When the mind bends - Issue
CC       198 of December 2017;
CC       URL="https://web.expasy.org/spotlight/back_issues/198/";
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DR   EMBL; KY984101; ASU62239.1; -; mRNA.
DR   AlphaFoldDB; P0DPA6; -.
DR   SMR; P0DPA6; -.
DR   KEGG; ag:ASU62239; -.
DR   GO; GO:0036469; F:L-tryptophan decarboxylase activity; IEA:UniProtKB-EC.
DR   GO; GO:0004609; F:phosphatidylserine decarboxylase activity; IEA:InterPro.
DR   GO; GO:0008654; P:phospholipid biosynthetic process; IEA:InterPro.
DR   GO; GO:0140380; P:psilocybin biosynthetic process; IDA:GO_Central.
DR   InterPro; IPR003817; PS_Dcarbxylase.
DR   InterPro; IPR022237; PsiD-like.
DR   PANTHER; PTHR10067; PTHR10067; 1.
DR   Pfam; PF02666; PS_Dcarbxylase; 1.
DR   Pfam; PF12588; PSDC; 1.
PE   1: Evidence at protein level;
KW   Decarboxylase; Lyase.
FT   CHAIN           1..439
FT                   /note="L-tryptophan decarboxylase"
FT                   /id="PRO_0000442157"
SQ   SEQUENCE   439 AA;  49655 MW;  C8798A98703AFFAA CRC64;
     MQVIPACNSA AIRSLCPTPE SFRNMGWLSV SDAVYSEFIG ELATRASNRN YSNEFGLMQP
     IQEFKAFIES DPVVHQEFID MFEGIQDSPR NYQELCNMFN DIFRKAPVYG DLGPPVYMIM
     AKLMNTRAGF SAFTRQRLNL HFKKLFDTWG LFLSSKDSRN VLVADQFDDR HCGWLNERAL
     SAMVKHYNGR AFDEVFLCDK NAPYYGFNSY DDFFNRRFRN RDIDRPVVGG VNNTTLISAA
     CESLSYNVSY DVQSLDTLVF KGETYSLKHL LNNDPFTPQF EHGSILQGFL NVTAYHRWHA
     PVNGTIVKII NVPGTYFAQA PSTIGDPIPD NDYDPPPYLK SLVYFSNIAA RQIMFIEADN
     KEIGLIFLVF IGMTEISTCE ATVSEGQHVN RGDDLGMFHF GGSSFALGLR KDCRAEIVEK
     FTEPGTVIRI NEVVAALKA