ID   PSID_PSICY              Reviewed;         439 AA.
AC   A0A286LEZ8;
DT   05-DEC-2018, integrated into UniProtKB/Swiss-Prot.
DT   22-NOV-2017, sequence version 1.
DT   25-MAY-2022, entry version 7.
DE   RecName: Full=L-tryptophan decarboxylase {ECO:0000303|PubMed:28763571};
DE            EC=4.1.1.105 {ECO:0000269|PubMed:30283667};
DE   AltName: Full=Psilocybin biosynthesis decarboxylase {ECO:0000303|PubMed:28763571};
GN   Name=psiD {ECO:0000303|PubMed:28763571};
OS   Psilocybe cyanescens.
OC   Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes;
OC   Agaricomycetidae; Agaricales; Strophariaceae; Psilocybe.
OX   NCBI_TaxID=93625;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], IDENTIFICATION, AND FUNCTION.
RC   STRAIN=FSU 12416;
RX   PubMed=28763571; DOI=10.1002/anie.201705489;
RA   Fricke J., Blei F., Hoffmeister D.;
RT   "Enzymatic synthesis of psilocybin.";
RL   Angew. Chem. Int. Ed. 56:12352-12355(2017).
RN   [2]
RP   FUNCTION, CATALYTIC ACTIVITY, AND PATHWAY.
RX   PubMed=30283667; DOI=10.1002/evl3.42;
RA   Reynolds H.T., Vijayakumar V., Gluck-Thaler E., Korotkin H.B.,
RA   Matheny P.B., Slot J.C.;
RT   "Horizontal gene cluster transfer increased hallucinogenic mushroom
RT   diversity.";
RL   Evol. Lett. 2:88-101(2018).
CC   -!- FUNCTION: L-tryptophan decarboxylase; part of the gene cluster that
CC       mediates the biosynthesis of psilocybin, a psychotropic tryptamine-
CC       derived natural product (PubMed:28763571, PubMed:30283667). The first
CC       step in the pathway is the decarboxylation of L-tryptophan to
CC       tryptamine by the decarboxylase psiD. PsiD does not decarboxylate
CC       phenylalanine, tyrosine, or 5-hydroxy- L -tryptophan (5-HTP)
CC       (PubMed:30283667). 4-hydroxy-L-tryptophan is accepted as substrate by
CC       psiD as well. The cytochrome P450 monooxygenase psiH then converts
CC       tryptamine to 4-hydroxytryptamine. The kinase psiK catalyzes the 4-O-
CC       phosphorylation step by converting 4-hydroxytryptamine into
CC       norbaeocystin. The methyltransferase psiM then catalyzes iterative
CC       methyl transfer to the amino group of norbaeocystin to yield psilocybin
CC       via a monomethylated intermediate, baeocystin. PsiK kinase can also
CC       turn psilocin into psilocybin. This activity may represent a protective
CC       mechanism to rephosphorylate the unstable psilocin to the stable
CC       psilocybin in case of intracellular ester cleavage (By similarity).
CC       {ECO:0000250|UniProtKB:P0DPA6, ECO:0000269|PubMed:28763571,
CC       ECO:0000269|PubMed:30283667}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H(+) + L-tryptophan = CO2 + tryptamine; Xref=Rhea:RHEA:30339,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57887,
CC         ChEBI:CHEBI:57912; EC=4.1.1.105;
CC         Evidence={ECO:0000269|PubMed:30283667};
CC   -!- PATHWAY: Secondary metabolite biosynthesis.
CC       {ECO:0000269|PubMed:30283667}.
CC   -!- SIMILARITY: Belongs to the phosphatidylserine decarboxylase family.
CC       {ECO:0000305}.
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DR   EMBL; KY984104; ASU62242.1; -; mRNA.
DR   AlphaFoldDB; A0A286LEZ8; -.
DR   GO; GO:0036469; F:L-tryptophan decarboxylase activity; IEA:UniProtKB-EC.
DR   GO; GO:0004609; F:phosphatidylserine decarboxylase activity; IEA:InterPro.
DR   GO; GO:0008654; P:phospholipid biosynthetic process; IEA:InterPro.
DR   GO; GO:0140380; P:psilocybin biosynthetic process; IDA:GO_Central.
DR   InterPro; IPR003817; PS_Dcarbxylase.
DR   InterPro; IPR022237; PsiD-like.
DR   PANTHER; PTHR10067; PTHR10067; 1.
DR   Pfam; PF02666; PS_Dcarbxylase; 1.
DR   Pfam; PF12588; PSDC; 1.
PE   1: Evidence at protein level;
KW   Decarboxylase; Lyase.
FT   CHAIN           1..439
FT                   /note="L-tryptophan decarboxylase"
FT                   /id="PRO_0000445827"
SQ   SEQUENCE   439 AA;  49758 MW;  B618E12573EB8AD9 CRC64;
     MQVLPACQSS ALKTLCPSPE AFRKLGWLPT SDEVYNEFID DLTGRTCNEK YSSQVTLLKP
     IQDFKTFIEN DPIVYQEFIS MFEGIEQSPT NYHELCNMFN DIFRKAPLYG DLGPPVYMIM
     ARIMNTQAGF SAFTKESLNF HFKKLFDTWG LFLSSKNSRN VLVADQFDDK HYGWFSERAK
     TAMMINYPGR TFEKVFICDE HVPYHGFTSY DDFFNRRFRD KDTDRPVVGG VTDTTLIGAA
     CESLSYNVSH NVQSLDTLVI KGEAYSLKHL LHNDPFTPQF EHGSIIQGFL NVTAYHRWHS
     PVNGTIVKIV NVPGTYFAQA PYTIGSPIPD NDRDPPPYLK SLVYFSNIAA RQIMFIEADN
     KDIGLIFLVF IGMTEISTCE ATVCEGQHVN RGDDLGMFHF GGSSFALGLR KDSKAKILEK
     FAKPGTVIRI NELVASVRK