PSIF_DICDI
ID PSIF_DICDI Reviewed; 708 AA.
AC Q7YSJ4; Q54YZ8;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2003, sequence version 1.
DT 25-MAY-2022, entry version 89.
DE RecName: Full=Protein psiF;
DE AltName: Full=Discoidin-inducing complex subunit A;
DE Flags: Precursor;
GN Name=psiF; Synonyms=dicA, dicA1; ORFNames=DDB_G0278581;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], PROTEIN SEQUENCE OF N-TERMINUS,
RP PROTEIN SEQUENCE OF 625-638, FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, AND
RP DEVELOPMENTAL STAGE.
RX PubMed=15947191; DOI=10.1128/ec.4.6.991-998.2005;
RA Kolbinger A., Gao T., Brock D., Ammann R., Kisters A., Kellermann J.,
RA Hatton D., Gomer R.H., Wetterauer B.;
RT "A cysteine-rich extracellular protein containing a PA14 domain mediates
RT quorum sensing in Dictyostelium discoideum.";
RL Eukaryot. Cell 4:991-998(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
CC -!- FUNCTION: Acts as a quorum sensing protein regulating discoidin gene
CC expression during growth and development. D.discoideum is a single-
CC celled amoebae and switches to multicellular development when food
CC becomes limited. As the growing cells reach a high density, they begin
CC expressing discoidin genes. The ability of psiF/dicA to induce
CC discoidin gene expression when present in conditioned medium, suggests
CC that it allow cells to sense their local density.
CC {ECO:0000269|PubMed:15947191}.
CC -!- SUBUNIT: Forms a complex with dicB. {ECO:0000269|PubMed:15947191}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass type I
CC membrane protein {ECO:0000305}. Secreted {ECO:0000269|PubMed:15947191}.
CC -!- DEVELOPMENTAL STAGE: Expressed in vegetative and developing cells.
CC {ECO:0000269|PubMed:15947191}.
CC -!- MISCELLANEOUS: According to PubMed:15947191, it is secreted in the
CC conditioned medium. However, prediction tools clearly predict a
CC transmembrane domain. In some conditions, it may be cleaved and
CC secreted in the conditioned medium, which would explain the
CC discrepancy.
CC -!- SIMILARITY: Belongs to the prespore-cell-inducing factor family.
CC {ECO:0000305}.
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DR EMBL; AJ548837; CAD69024.1; -; mRNA.
DR EMBL; AJ548838; CAD69025.1; -; Genomic_DNA.
DR EMBL; AAFI02000023; EAL68463.1; -; Genomic_DNA.
DR RefSeq; XP_642054.1; XM_636962.1.
DR AlphaFoldDB; Q7YSJ4; -.
DR PaxDb; Q7YSJ4; -.
DR EnsemblProtists; EAL68463; EAL68463; DDB_G0278581.
DR GeneID; 8621266; -.
DR KEGG; ddi:DDB_G0278581; -.
DR dictyBase; DDB_G0278581; psiF.
DR eggNOG; ENOG502RA06; Eukaryota.
DR HOGENOM; CLU_024170_0_0_1; -.
DR InParanoid; Q7YSJ4; -.
DR OMA; TCSYYDY; -.
DR PhylomeDB; Q7YSJ4; -.
DR PRO; PR:Q7YSJ4; -.
DR Proteomes; UP000002195; Chromosome 3.
DR GO; GO:0005576; C:extracellular region; IDA:dictyBase.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR InterPro; IPR011874; Fibro_Slime.
DR InterPro; IPR037524; PA14/GLEYA.
DR InterPro; IPR011658; PA14_dom.
DR InterPro; IPR001673; S_mold_repeat.
DR Pfam; PF00526; Dicty_CTDC; 3.
DR Pfam; PF07691; PA14; 1.
DR SMART; SM00758; PA14; 1.
DR TIGRFAMs; TIGR02148; Fibro_Slime; 1.
DR PROSITE; PS51820; PA14; 1.
PE 1: Evidence at protein level;
KW Developmental protein; Differentiation; Direct protein sequencing;
KW Glycoprotein; Membrane; Reference proteome; Secreted; Signal;
KW Transmembrane; Transmembrane helix.
FT SIGNAL 1..19
FT /evidence="ECO:0000269|PubMed:15947191"
FT CHAIN 20..708
FT /note="Protein psiF"
FT /id="PRO_0000327558"
FT TOPO_DOM 20..643
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 644..664
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 665..708
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT DOMAIN 103..263
FT /note="PA14"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01164"
FT REGION 682..708
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 78
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 116
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 222
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 317
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 318
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 371
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 498
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 600
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 708 AA; 76482 MW; CEEC0F6A5F7B1190 CRC64;
MKYLFIAIIL ILYCSFTKAD QKKFLVNMYD NDPLFSPDFE NANGAQTGLV KKKLGSDGKP
IPANYDMKDP NGNYYIKNAT TFKSWFNEVA GVSILVPFEL VLTQTAGSQN YYSYSNTSFF
PLNELGWYNP SIKGDYEFKK YQDSNKKEQN FHFCMHASFI MSTNCKEVFK FKGDDDVWVF
INDVLVLDIG GVHGVQDGTV DMANLPEKIH DSTNSKLGNC KNGTYPFDFF YCERHTKASN
CLFETNMGFT CSYYDYCGIC NGKGECCTDV KLNQCYTKKC PLPNSLPNGA TNYQDYMTIV
PTNTCGGTDK CKIYSCNNST GCEFKQKSCD DGDKCTKDAC DSKTGYCSNI PTNPSVVTSC
LKSGCDSTTG NYSTPTNCDD KDPCTIDSCI NGQGCVHTKA CDDEDPCTTD SCSADGKCTH
TAIAKCNSDC PSCPSKKCKI TSCSEDSGAC NYVDMVFASP SECYKATCDP ETEEAIYSPI
DSSCDTSDSC FTAQCNLNKT CTRVPAINCD DNNECTTDSC SGGSCSNTAI ACDDNDPCTI
DTCSPSEGCI FTPIVCEQTS LCNTFTCSVG KCVPTPITCS SSVKCQDSIC REGVGCVYFN
RTCPPDDDCS SAYCSMETGK CISKAYDPLP FSCQSTAVKV GVGIGAAAAA GIAIGGAVAA
GLAIFGGKKA YDTWKTSRGN VMTGSQSNPL YTQNQNNGNN PLYSAPAE
