ID   PSIR_PSICU              Reviewed;         358 AA.
AC   P0DPB0;
DT   25-OCT-2017, integrated into UniProtKB/Swiss-Prot.
DT   25-OCT-2017, sequence version 1.
DT   25-MAY-2022, entry version 13.
DE   RecName: Full=Psilocybin cluster transcription regulator {ECO:0000303|PubMed:28763571};
GN   Name=psiR {ECO:0000303|PubMed:28763571};
OS   Psilocybe cubensis (Psychedelic mushroom) (Stropharia cubensis).
OC   Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes;
OC   Agaricomycetidae; Agaricales; Strophariaceae; Psilocybe.
OX   NCBI_TaxID=181762;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], IDENTIFICATION, AND FUNCTION.
RX   PubMed=28763571; DOI=10.1002/anie.201705489;
RA   Fricke J., Blei F., Hoffmeister D.;
RT   "Enzymatic synthesis of psilocybin.";
RL   Angew. Chem. Int. Ed. 56:12352-12355(2017).
CC   -!- FUNCTION: Transcription factor that may regulate the expression of the
CC       gene cluster that mediates the biosynthesis of psilocybin, a
CC       psychotropic tryptamine-derived natural product (PubMed:28763571).
CC       {ECO:0000305|PubMed:28763571}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00981}.
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DR   EMBL; MF000990; ASU62243.1; -; Genomic_RNA.
DR   AlphaFoldDB; P0DPB0; -.
DR   SMR; P0DPB0; -.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR   Gene3D; 4.10.280.10; -; 1.
DR   InterPro; IPR011598; bHLH_dom.
DR   InterPro; IPR036638; HLH_DNA-bd_sf.
DR   Pfam; PF00010; HLH; 1.
DR   SMART; SM00353; HLH; 1.
DR   SUPFAM; SSF47459; SSF47459; 1.
DR   PROSITE; PS50888; BHLH; 1.
PE   3: Inferred from homology;
KW   Coiled coil; DNA-binding; Nucleus; Transcription; Transcription regulation.
FT   CHAIN           1..358
FT                   /note="Psilocybin cluster transcription regulator"
FT                   /id="PRO_0000442161"
FT   DOMAIN          199..249
FT                   /note="bHLH"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT   REGION          1..40
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          62..212
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          199..212
FT                   /note="Basic motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT   REGION          213..249
FT                   /note="Helix-loop-helix motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT   REGION          308..358
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          264..306
FT                   /evidence="ECO:0000255"
FT   COMPBIAS        73..93
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        139..165
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        194..212
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   358 AA;  37706 MW;  A182EE54925D7042 CRC64;
     MAPATPATHD PALSHGAPPA PGAPAPANAP PNASGDIAGM QLSGLDQSQI MNLLRSLPGM
     FSGGKIPDQG QGNKEDAAQT LSNLAQAQPY GQQLPLHYQA GGPGGLPGIN DPGPSTHPRG
     PPNLGQLSAV AMQAAPAPIQ HPDQQTNRND GEQAGNASAS TSGKDGDNAE FVPPPAPAPT
     TGRRGGRSAT MGSDEWSRQR KDNHKEVERR RRGNINEGIN ELGRIVPSGS GEKAKGAILS
     RAVQYIHHLK ENEARNIEKW TLEKLLMDQA MGDLQAQLEE VKRLWEEERM ARTRLEAELE
     VLRNMNGVNA GSAPASKDES AAGTKRRSTD GAEAATAATE SSTANAEGER DGKRQRTE